ESS - MN - Posters apresentados em eventos científicos
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- 3D vs 2D Cell Cultures in the Evaluation of Radiobiological Effects of Exposition to Low Doses - Medical Imaging Levels - of Ionizing RadiationPublication . Costa, Pedro; Caires, Hugo; Lemos, Joana; Cunha, Lídia; Bravo, Joana; Bravo, Isabel; Silva, Regina; Summavielle, Teresa; Metello, Luís F.Pretending to develop advanced biological models to study biological effects of low doses of ionizing radiation and following the actual policies on Animal Sciences, based on 3 R’s Rule (to Reduce, Refine and Replace) – that limits as much as possible the application of animal models – scientific research using cellular models is constantly increasing. Nevertheless, the intrinsic limitations of actual cellular models quite often had been recognized on a significant number of papers pointing a significant number of non-concordances between results obtained using in vitro and in vivo studies. Actually, an increasing number of authors admit that three-dimensional cell culture (and spheroid cell culture in particular) could represent an interesting solution and a step further on use of cellular models. The work here to be presented reflects the first phase on the use of this methodology on the study, evaluation and quantification of cellular effects of low doses – starting on medical imaging level - of exposition to ionizing radiation.
- 99mTc-MAA for Lung Perfusion Scintigraphy: Impact of Counting Method on the Effective Number of Particles Administered and Particle Aggregation Over TimePublication . Costa, Pedro; Cunha, Lídia; Soares, Sofia; Mendes, Ângela; Queiroz, Ana; Nunes, Amélia; Ferreira, Elisabete; Botelho, Elisa; Sousa, Maria C.; Castro, Rosa; Pires, Laucena; João, M. Faria; Metello, Luís F.: 99mTc-MAA (macroaggregates of albumin) is the selected radiopharmaceutical for the Lung Perfusion Scintigraphy. It is retained in lung capillaries by mechanical blockage resulting in local microembolization. Care should be taken concerning not only particle size but also the number of particles administered to patients, particularly those who present with special conditions (pulmonary hypertension, shunt or lung transplant). European Pharmacopoeia only requires the assessment of particle size. This work aims to characterize particle size and particle aggregation of two commercial brands of MAA kits and to compare particle counting methods
- 99mTc-MAA for Lung Perfusion Scintigraphy: Impact of Counting Method on the Effective Number of Particles Administered and Particle Aggregation Over TimePublication . Costa, Pedro; Cunha, Lídia; Soares, Sofia; Mendes, Ângela; Queiroz, A. Isabel; Nunes, Amélia; Ferreira, Elisabete; Botelho, Elisa; Sousa, Conceição; Castro, Rosa; Pires, Laucena; João, M. Faria; Metello, Luís F.Introduction: 99mTc-MAA (macroaggregates of albumin) is the selected radiopharmaceutical for the Lung Perfusion Scintigraphy. It is retained in lung capillaries by mechanical blockage resulting in local microembolization. Care should be taken concerning not only particle size butalso the number of particles administered to patients, particularly those who present with special conditions (pulmonary hypertension, shunt or lung transplant). European Pharmacopoeia only requires the assessment of particle size. This work aims to characterize particle size and particle aggregation of two commercial brands of MAA kits and to compare particle counting methods.
- Acetyl-L-Carnitine Improves Cell BioenergeticsPublication . Cunha, Lídia; Bravo, Joana; Costa, Pedro; Fernandes, Sílvia; Oliveira, Marta; Castro, Rosa; Metello, Luís F.; Summavielle, TeresaIntroduction: Acetyl-L-Carnitine (ALC), a natural occurring compound in all mammalian species, plays a variety of vital functions in the body. The most important are related to mitochondria, namely the transport of fatty acids for energy production through β oxidation and the control of acyl-CoA/CoA ratio. Due to this close interaction with cell bioenergetics, it plays a role in many diseases, especially those related to the mitochondria. We propose to characterize the action of ALC in mitochondrial bioenergetics and functional integrity.
- Actualities on Nuclear Medicine Applications in Sports MedicinePublication . Fonseca, Abigaíl; Soares, Sofia; Cunha, Lídia; Metello, Luís F.Aims: This paper aims to address some of the main possible applications of actual Nuclear Medicine Imaging techniques and methodologies in the specific context of Sports Medicine, namely in two critical systems: musculoskeletal and cardiovascular. Discussion: At the musculoskeletal level, bone scintigraphy techniques proved to be a mean of diagnosis of functional orientation and high sensibility compared with other morphological imaging techniques in the detection and temporal evaluation of pathological situations, for instance allowing the acquisition of information of great relevance in athletes with stress fractures. On the other hand, infection/inflammation studies might be of an important added value to characterize specific situations, early diagnose of potential critical issues – so giving opportunity to precise, complete and fast solutions – while allowing the evaluation and eventual optimization of training programs. At cardiovascular system level, Nuclear Medicine had proved to be crucial in differential diagnosis between cardiac hypertrophy secondary to physical activity (the so called "athlete's heart") and hypertrophic cardiomyopathy, in the diagnosis and prognosis of changes in cardiac function in athletes, as well as in direct - and non-invasive - in vivo visualization of sympathetic cardiac innervation, something that seems to take more and more importance nowadays, namely in order to try to avoid sudden death episodes at intense physical effort. Also the clinical application of Positron Emission Tomography (PET) has becoming more and more widely recognized as promising. Conclusions: It has been concluded that Nuclear Medicine can become an important application in Sports Medicine. Its well established capabilities to early detection of processes involving functional properties allied to its high sensibility and the actual technical possibilities (namely those related with hybrid imaging, that allows to add information provided by high resolution morphological imaging techniques, such as CT and/or MRI) make it a powerful diagnostic tool, claiming to be used on an each day higher range of clinical applications related with all levels of sport activities. Since the improvements at equipment characteristics and detection levels allows the use of smaller and smaller doses, so minimizing radiation exposure it is believed by the authors that the increase of the use of NM tools in the Sports Medicine area should be considered.
- ALC Neuroprotection through autophagy and ups acitivityPublication . Bravo, Joana; Cunha, Lídia; Fernandes, Sílvia; Binienda, Zbigniew; Summavielle, TeresaAcetyl–L-carnitine (ALC) has beneficial effects in neurodegenerative diseases and was shown to be protective against exposure to methamphetamine (METH) reducing mitochondrial dysfunction and oxidative stress. However, the mechanisms underlying ALC action are still unknown, limiting its putative therapeutic use.
- An in vivo evaluation of the potential of 188Re-PEI-MP for therapy of bladder carcinoma and 99mTc-PEI-MP for diagnosis and follow upPublication . Ferreira, Sara; Laranjo, Mafalda; Abrantes, Ana Margarida; Brito, Ana; Pires, Salomé; Metello, Luís F.; Zeevart, Jan; Louw, Werner; Dormehl, Irene; Botelho, Maria FilomenaThe development of water-soluble polymers such as PEI-MP (polyethyleneimine, functionalised with methylphosphonate groups) that might be labeled with 188Re (emits of high energy beta particles) and 99mTc are recent approaches, with a strong potential for metabolic radiotherapy and diagnosis, respectively. The aim of this study was to evaluate the efficacy of 188Re-PEI-MP as therapeutic agent for bladder carcinoma and 99mTc-PEI-MP for its diagnosis and follow up.
- An in vivo study to analyse the potential of 188Re-PEI-MP for metabolic radiotherapy of bladder carcinomaPublication . Ferreira, Sara; Laranjo, Mafalda; Abrantes, Ana Margarida; Brito, Ana; Metello, Luís F.; Zeevart, Jan; Louw, Werner; Dormehl, Irene; Botelho, Maria Filomena188Re is a promising radionuclide for metabolic therapy because of the emission of high energy beta-particles. The development of water-soluble polymers such as PEI-MP (polyethyleneimine, functionalised with methylphosphonate groups) that might be labeled with 188Re are recent approaches, with a strong potential for metabolic radiotherapy. The aim of this study was to evaluate the efficacy of 188Re-PEI-MP, as therapeutic agent for bladder carcinoma
- An in vivo study to analyse the potential of 188Re-PEI-MP for metabolic radiotherapy of osteosarcoma and bladder carcinomaPublication . Ferreira, Sara; Laranjo, Mafalda; Brito, Ana; Abrantes, Ana Margarida; Metello, Luís F.; Zeevart, Jan; Louw, Werner; Dormehl, Irene; Botelho, Maria FilomenaIntroduction: 188Re is a promising radionuclide for metabolic therapy because of the emission of high energy beta-particles. The development of water-soluble polymers such as PEI-MP (polyethyleneimine, functionalised with methylphosphonate groups) that might be labeled with 188Re are recent approaches, with a strong potential for metabolic radiotherapy.
- Analysing the usefulness of motion correction software in myocardial perfusion imagingPublication . Cunha, Lídia; Lamego, Juliana; Ferreira, Sara; Lemos, Joana; Vieira, Domingos; Metello, Luís F.Introduction Myocardial Perfusion Imaging (MPI) is a very important tool in the assessment of Coronary Artery Disease ( CAD ) patient s and worldwide data demonstrate an increasingly wider use and clinical acceptance. Nevertheless, it is a complex process and it is quite vulnerable concerning the amount and type of possible artefacts, some of them affecting seriously the overall quality and the clinical utility of the obtained data. One of the most in convenient artefacts , but relatively frequent ( 20% of the cases ) , is relate d with patient motion during image acquisition . Mostly, in those situations, specific data is evaluated and a decisi on is made between A) accept the results as they are , consider ing that t he “noise” so introduced does not affect too seriously the final clinical information, or B) to repeat the acquisition process . Another possib ility could be to use the “ Motion Correcti on Software” provided within the software package included in any actual gamma camera. The aim of this study is to compare the quality of the final images , obtained after the application of motion correction software and after the repetition of image acqui sition. Material and Methods Thirty cases of MPI affected by Motion Artefacts and repeated , were used. A group of three, independent (blinded for the differences of origin) expert Nuclear Medicine Clinicians had been invited to evaluate the 30 sets of thre e images - one set for each patient - being ( A) original image , motion uncorrected , (B) original image, motion corrected, and (C) second acquisition image, without motion . The results so obtained were statistically analysed . Results and Conclusion Results obtained demonstrate that the use of the Motion Correction Software is useful essentiall y if the amplitude of movement is not too important (with this specific quantification found hard to define precisely , due to discrepancies between clinicians and other factors , namely between one to another brand); when that is not the case and the amplitude of movement is too important , the n the percentage of agreement between clinicians is much higher and the repetition of the examination is unanimously considered ind ispensable.