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Advisor(s)
Abstract(s)
Pretending to develop advanced biological models to study biological effects of
low doses of ionizing radiation and following the actual policies on Animal Sciences,
based on 3 R’s Rule (to Reduce, Refine and Replace) – that limits as much as possible
the application of animal models – scientific research using cellular models is
constantly increasing. Nevertheless, the intrinsic limitations of actual cellular models
quite often had been recognized on a significant number of papers pointing a
significant number of non-concordances between results obtained using in vitro and in
vivo studies. Actually, an increasing number of authors admit that three-dimensional
cell culture (and spheroid cell culture in particular) could represent an interesting
solution and a step further on use of cellular models. The work here to be presented
reflects the first phase on the use of this methodology on the study, evaluation and
quantification of cellular effects of low doses – starting on medical imaging level - of
exposition to ionizing radiation.