ESS - MN - Posters apresentados em eventos científicos
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- Assessing left ventricular dysfunction by the use of three distinct molecular imaging techniquesPublication . Reis, J.; Cunha, L.; Costa, Pedro; Neves, D.; Oliveira, T.; Ferrer-Antunes, A.; João, M. Faria; Metelo, Luís FranciscoLeft ventricular dysfunction might be assessed using distinct molecular imaging modalities. The most relevant are first pass radionuclide ventriculography (FPRV), multigated radionuclide angiography (MUGA) and gated blood-pool SPECT (GBPS) and are very often used for monitoring chemotherapy-related cardiomyopathy. This work aims to correlate these three molecular imaging techniques.
- Evaluation of 18F-FDG PET/CTand low-dose CT performance in diagnosing lymph node metastases among NSCLC patientsPublication . Teodorczyk, J.; Brockhuis, B.; Romanowicz, G.; Cytawa, W.; Wenzel, I.; Kozłowska, J.; Cunha, L.; Silva, A.; Metelo, Luís; Lass, P.The aim of our study is to assess the efficacy of lymph node staging in NSCLC, with particular emphasis on operability, using 18F-FDG-PET-CT and low-dose CT.
- Assessing radiobiological effects of low doses of ionizing radiation on zebrafish muscle by two-dimensional gel electrophoresisPublication . Lemos, Joana; Campos, A.; Carneiro, M.; Ribeiro, T.; Ponte, F.; Costa, Pedro; Cunha, L.; Carvalho, A.; Metelo, Luís FranciscoThis work relates with the application of zebrafish - Danio rerio - to the study of radiobiological effects of low doses of ionizing radiation. In recent years, the use of zebrafish has grown considerably, pointing more and more as a very interesting model in biomedical research, essentially because of the level of homology shared with the human genome, complemented by an easy and reasonably affordable practical side.
- Can potassium perchlorate and sodium iodine influence Iodine-131 uptake in Cholangiocarcinoma?Publication . Brito, A. F.; Puga, T.; Teixo, R.; Fernandes, A.; Brito, A. F.; Abrantes, A. M.; Metelo, Luís Francisco; Tralhão, J. G.; Botelho, M. F.Cholangiocarcinoma (CC) is a malignancy with poor prognostic, high mortality and limited treatment options, reason why the development of novel treatment strategies by exploiting new targets might be considered as consensually relevant. In fact, it was shown that CC expressed the sodium iodine symporter (NIS), which is responsible for iodine uptake by the cells. NIS is also the responsible for the uptake of iodine-131 (131I) in thyroid tumor and benign diseases, enabling the metabolic radiotherapy and opening possibilities of new treatment approaches for CC.
- Internal audits: an ally in the promotion of a radiation protection culturePublication . Cunha, L.; Roda, A. L.; Ratão, P.; Capoulas, M.; Ferreira, F.; Sousa, M.; Vasconcelos, H.; Moreira, A. S.; Metelo, Luís FranciscoInternal audits are between the most important tools supporting the management board of any organization. Regardless if they are conducted by na internal or an external auditor, the goals are to assist the organization in defining areas of improvement, while providing precise information needed to accomplish the established goals. We aim to contribute to improving the community awareness of the value of conducting regular audits and its impact on promoting a culture of radiation protection.
- The novel coronavirus: Our experience in facing an invisible enemyPublication . Cunha, L.; Ratão, P.; Roda, A.; Capoulas, M.; Ferreira, F.; Sousa, M.; Vasconcelos, H.; Moreira, A. S.; Metelo, Luís FranciscoEarly this year, Chinese authorities identified a new type of coronavirus that was causing a disease named COVID-19. It disseminated quickly, and immediate action was needed to control the further spread of the infection.
- Desenvolvimento e aplicação de modelos biológicos para estudo de efeitos da radiação ionizantePublication . Lemos, Joana; Vieira, Domingos; Costa, PedroCom o crescente uso médico das radiações ionizantes, tanto ao nível dos meios complementares de diagnóstico (ex: Medicina Nuclear e Radiologia), como ao nível terapêutico (ex: Radioterapia), as preocupações inerentes ao aumento da exposição da população a este tipo de radiações têm aumentado. Apesar dessas preocupações, a literatura carece ainda de evidência científica suficiente para esclarecer a relação entre a irradiação com baixas doses de radiação ionizante e os efeitos despoletados.
- PET aplicada à Medicina Moderna: prova de conceito baseada na produção de Titânio-45 ( 45Ti)Publication . Costa, Pedro; Alves, F.; Naia, M. DuarteOs paradigmas modernos da Medicina baseada em Evidência e/ou Ciência, bem como os conceitos relacionados com a Medicina Personalizada, têm levado ao crescente uso de meios complementares de diagnóstico, cada vez mais sensíveis específicos. Nesse contexto, as metodologias de imagem médica têm especial relevo .Dentro destas, vários exemplos podem ser destacados, mas a Medicina Nuclear, baseada no uso de radiofármacos, é especialmente importante ao nível do estudo funcional e molecular dos mais diversos processos patológicos.
- Effect of umbilical cord mesenchymal stem cells secretome in melanomaPublication . Accioly, Gustavo; Aguiar, Gonçalo; Areal, Lara; Costa, Pedro; Coelho, Pedro; Gomes, AndreiaMelanoma of the skin is one of the most prominent and fastest growing malignancies. More than 300 thousand diagnosed cases and 57 thousand deaths in 2020 worldwide and roughly 517 thousand cases of were registered during the 2015-2020 period. Melanoma is generally regarded as an aggressive and unpredictable cancer whose conventional therapies, such as local excision, chemotherapy and immunotherapy, have encountered difficulties to prevent larger scale-tumours and metastasis, as well as overcome recurrence and development of drug resistance. Stem cellbased therapies have been studied as interesting therapeutical approaches for cancer whenever conventional therapy fails to impede its progression. That is owing to the anti-proliferative and immunomodulatory capacity of some SC, being one of the major examples, Mesenchymal Stem Cells. Due to its high abundance, well defined extraction and expansion protocols as well as documented anti-tumorigenic characteristics, Umbilical Cord derived Mesenchymal Stem Cells (UC-MSC) have been observed as a promising candidate for Melanoma treatment, specially through acellular therapy using its secretome. MSC secretome is defined as the set of MSCs-derived bioactive factors available extracellularly and is responsible for the major therapeutic effects of MSCs, namely in oncological pathologies. In this study we hypothesize the ability of UC-MSC’s secretome to inhibit Melanoma growth in vitro. UC-MSC secretome, in the form of conditioned medium (CM), was obtained by extraction from selected umbilical cords and expansion of while murine melanoma cell line B16-F10 culture was established. After treating melanoma cells with different concentrations of CM (100%, 50% and 25%), common cancer hallmarks such as cell viability, motility, colony formation and cell interactions were assessed through MTT, Wound Healing and Colony formation and Hanging-Drop assays, respectively. General analysis of viability and motility showed no statistically significant difference between treated and control groups as well as no concentration-dependent effect whereas formation of cellular aggregates follows an inhibition trend on the treated groups. These results put into perspective the effect of secretome of UC-MSCs. Moreover, further larger scale studies are needed for deeper understanding of MSC secretome mechanisms of action, therefore enabling their use in acellular therapies against melanoma in the future.
- Possible modulation of dopaminergic neurotransmission function by acetyl-L-carnitinePublication . Cunha, L.; Szigeti, K.; Mathe, D.; Summavielle, T.; Metello, L.Acetyl‐L‐Carnitine (ALC) has a putative neuroprotective effect being used in a variety of conditions. Nevertheless, the underlying molecular mechanisms, particularly regarding the induction of changes in neurotransmitter systems, are still not fully understood. We aim to contribute for the elucidation of the mechanisms by which ALC alters neurotransmitter release, using a cell line and an animal model of exposure to methamphetamine (METH). PC12 cells were incubated with several doses of ALC (0.01 to 1.0 mM) alone or in combination with METH 1.0 or 100 µM for 24h or 72h. When combined, ALC preceded METH administration in 30 minutes. Dopamine (DA) content was determined by high performance liquid chromatography. C57BL/6J mice were used for in vivo assays to assess DA striatal binding. Mice were divided into 4 groups, according to different treatments: group 1 (control), group 2 (ALC, 100 mg/kg), group 3 (METH, 10 mg/kg) and group 4 (ALC+METH). Images were acquired in a SPECT/CT scanner (NanoSPECT/CT, Mediso, Hungary) 70 minutes after 123I‐IBZM injection. Regions of interest were drawn in the striata and in the cerebellum to determine the striatal binding ratio. Increased intracellular levels of DA were observed in PC12 cells at 24h and 72h after the administration of ALC. Cells treated with METH 100 µM displayed decreased intracellular levels of DA. ALC prevented the METH‐induced decrease in DA concentration (p<0.0001). On the other hand, a single dose of 10 mg/kg of METH induced a decrease in striatal D2R binding ratios comparing to control group (between 20% and 30%). Interestingly, over time, ALC was able to reverse the decrease on the radiotracer binding induced by METH. The present study indicates a possible effect of ALC over METH‐induced DA release.