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- O uso de Ferramentas Tecnológicas no Ensino Superior Moderno: abordagem integrada e perspectivas de aplicação em caso práticoPublication . Vieira, Domingos; Costa, Pedro; Cunha, Lídia; Lemos, Joana; Ferreira, Sara; Metello, Luís F.A utilização de ferramentas tecnológicas no Ensino Superior como metodologia de ensino/aprendizagem apresenta-se cada vez mais generalizada. Consciente do facto, a Área Técnico-Científica de Medicina Nuclear da ESTSP.IPP, tem vindo a evoluir na integração gradual de algumas ferramentas tecnológicas na sua realidade específica quotidiana, sempre no sentido de aumentar a qualidade dos serviços que presta e a eficiência do processo, encarado na sua globalidade, acreditando na importante contribuição que podem vir a representar em termos de aumento da competitividade e da facilitação do alcance dos objectivos assumidos, particularmente em termos de ensino à distância e de internacionalização dos diversos projectos pedagógicos em que esta Área se encontra envolvida. Este trabalho tem como objectivo partilhar a experiência adquirida e o estado actual do uso de ferramentas tecnológicas no quotidiano, assim como os próximas etapas previstas no desenvolvimento de este projecto, considerado, por definição, como de longo-prazo. Serão apresentadas uma série de considerações, particularmente em termos da eficácia, inerentes à utilização quotidiana das várias ferramentas que foram já implementadas, sendo ainda tecidas uma série de recomendações e alertas, decorrentes da nossa experiência prática e potencialmente úteis aos que se estão a (ou pretendem) iniciar. O Espaço Europeu do Ensino Superior é declaradamente um desafio major para o qual temos que nos preparar da forma mais eficaz e competitiva, acreditando que a adopção de ferramentas tecnológicas – de uma forma atempada e sobretudo adequada aos objectivos específicos concretos de cada projecto pedagógico – pode vir a constituir um factor decisivo em termos do respectivo sucesso.
- 3D vs 2D Cell Cultures in the Evaluation of Radiobiological Effects of Exposition to Low Doses - Medical Imaging Levels - of Ionizing RadiationPublication . Costa, Pedro; Caires, Hugo; Lemos, Joana; Cunha, Lídia; Bravo, Joana; Bravo, Isabel; Silva, Regina; Summavielle, Teresa; Metello, Luís F.Pretending to develop advanced biological models to study biological effects of low doses of ionizing radiation and following the actual policies on Animal Sciences, based on 3 R’s Rule (to Reduce, Refine and Replace) – that limits as much as possible the application of animal models – scientific research using cellular models is constantly increasing. Nevertheless, the intrinsic limitations of actual cellular models quite often had been recognized on a significant number of papers pointing a significant number of non-concordances between results obtained using in vitro and in vivo studies. Actually, an increasing number of authors admit that three-dimensional cell culture (and spheroid cell culture in particular) could represent an interesting solution and a step further on use of cellular models. The work here to be presented reflects the first phase on the use of this methodology on the study, evaluation and quantification of cellular effects of low doses – starting on medical imaging level - of exposition to ionizing radiation.
- Efeitos Biológicos das Radiações IonizantesPublication . Lemos, Joana; Metello, Luís F.
- Study of the Influence of Patient Hydration in Bone ScintigraphyPublication . Ferreira, Sara; Cunha, Lídia; Osório, Soraia; Matias, Mariana; Lemos, Joana; Vieira, Domingos; Soares, Sofia; Fonseca, Abigaíl; Silva, José Alexandre; Amorim, Inês; Castro, Rosa; Metello, Luís F.The bone scintigraphy is a diagnosis method noninvasive and sensitive in detecting early bone lesions, constituting about 35% of all tests performed in the Service of Nuclear Medicine, of the Hospital of Santo Antonio, in the city of Porto, where the following study took place. One of the important technical details in preparing the patient for this examination is the hydration following the administration of the radiopharmaceutical. The aim of this study focused on assessing the value of hydration on the quality of the image in a bone scintigraphy.
- Is there a need for attenuation correction in the 99mTc-DMSA scans on pediatric patients?Publication . Nogueira, Fábio; Lemos, Joana; Oliveira, Marta; Silva, José A.; Sousa, Maria C.; Cunha, Lídia; Metello, Luís F.Introduction: The quantification of th e differential renal function in adults can be difficult due to many factors - on e of the se is the variances in kidney depth and the attenuation related with all the tissue s between the kidney and the camera. Some authors refer that t he lower attenuation i n p ediatric patients makes unnecessary the use of attenuation correction algorithms. This study will com pare the values of differential renal function obtained with and with out attenuation correction techniques . Material and Methods: Images from a group consisting of 15 individuals (aged 3 years +/ - 2) were used and two attenuation correction method s were applied – Tonnesen correction factors and the geometric mean method . The mean time of acquisition (time post 99m Tc - DMSA administration) was 3.5 hours +/ - 0.8h. Results: T he absence of any method of attenuation correction apparently seems to lead to consistent values that seem to correlate well with the ones obtained with the incorporation of methods of attenuation correction . The differences found between the values obtained with and without attenuation correction were not significant. Conclusion: T he decision of not doing any kind of attenuation correction method can apparently be justified by the minor differences verified on the relative kidney uptake values. Nevertheless, if it is recognized that there is a need for a really accurate value of the relative kidney uptake, then an attenuation correction method should be used.
- Preclinical Imaging: an Essential Ally in Modern BiosciencesPublication . Cunha, Lídia; Horvath, Ildiko; Ferreira, Sara; Lemos, Joana; Costa, Pedro; Vieira, Domingos; Veres, Dániel S.; Szigeti, Krisztián; Summavielle, Teresa; Máthé, Domokos; Metello, Luís F.Translational research is changing the practice of modern medicine and the way in which health problems are approached and solved. The use of small-animal models in basic and preclinical sciences is a major keystone for these kinds of research and development strategies, representing a bridge between discoveries at the molecular level and clinical implementation in diagnostics and/or therapeutics. The development of high-resolution in vivo imaging technologies provides a unique opportunity for studying disease in real time, in a quantitative way, at the molecular level, along with the ability to repeatedly and non-invasively monitor disease progression or response to treatment. The greatest advantages of preclinical imaging techniques include the reduction of biological variability and the opportunity to acquire, in continuity, an impressive amount of unique information (without interfering with the biological process under study) in distinct forms, repeated or modulated as needed, along with the substantial reduction in the number of animals required for a particular study, fully complying with 3R (Replacement, Reduction and Refinement) policies. The most suitable modalities for small-animal in vivo imaging applications are based on nuclear medicine techniques (essentially, positron emission tomography [PET] and single photon emission computed tomography [SPECT]), optical imaging (OI), computed tomography (CT), magnetic resonance imaging (MRI), magnetic resonance spectroscopy imaging (MRSI), and ultrasound. Each modality has intrinsic advantages and limitations. More recently, aiming to overcome the inherent limitations of each imaging modality, multimodality devices designed to provide complementary information upon the pathophysiological process under study have gained popularity. The combination of high-resolution modalities, like micro-CT or micro-MRI, with highly sensitive techniques providing functional information, such as micro-PET or micro-SPECT, will continue to broaden the horizons of research in such key areas as infection, oncology, cardiology, and neurology, contributing not only to the understanding of the underlying mechanisms of disease, but also providing efficient and unique tools for evaluating new chemical entities and candidate drugs. The added value of small-animal imaging techniques has driven their increasing use by pharmaceutical companies, contract research organizations, and research institutions.
- In the Way to ISO 9001:2008: Obtaining the Quality Certification in Public Higher EducationPublication . Metello, Luís F.; Cunha, Lídia; Lemos, Joana; Lourenço, Leonel; Ferreira, Sara; Nogueira, Fábio; Vieira, Domingos; Pinto, HelenaQuality Management in Higher Education is a rather complex and each day more challenging process. Nevertheless, it is believed to soon become the cornerstone for the development of modern Higher Education, so with an increasing role on Society itself; the clear assumption of this might be the – so needed - turning point in the relations between Academy and the “Real World”, helping to fill the gap.
- Actual Trends in Nuclear MedicinePublication . Vieira, Domingos; Lemos, Joana; Ferreira, Sara; Metello, Luís F.
- Assessing Radiobiological Effects of Low Doses of Ionizing Radiation on Zebrafish by Bidimensional Gel ElectrophoresisPublication . Lemos, Joana; Costa, Pedro; Cunha, Lídia; Campos, Alexandre; Carvalho, António Paulo; Vasconcelos, Vítor; Genésio, Paula; Ponte, Fernanda; Costa, Paulo; Crespo, Paulo; Metello, Luís F.Aim: This paper relates with a specific task from a larger research project oriented to the development and application of the Zebrafish – Danio rerio – to the study of radiobiological effects of medical diagnostic level – low doses – of ionizing radiation.
- Assessing radiobiological effects of low doses of ionizing radiation on zebrafish muscle by two-dimensional gel electrophoresisPublication . Lemos, Joana; Campos, Alexandre; Carneiro, Mariana; Ribeiro, Tiago; Ponte, Fernanda; Costa, Paulo; Cunha, Lídia; Carvalho, António Paulo; Metello, Luís F.Introduction: This work relates with the application of zebrafish - Danio rerio - to the study of radiobiological effects of low doses of ionizing radiation. In recent years, the use of zebrafish has grown considerably, pointing more and more as a very interesting model in biomedical research, essentially because of the level of homology shared with the human genome, complemented by an easy and reasonably affordable practical side. The two-dimensional gel electrophoresis (2DGE) is a method used for the analysis of complex protein mixtures from biological samples. This method allows the detection of differences in protein expression under different conditions, so allowing creating relations between those differences and the irradiations that were in their basis. The present work aimed at evaluating proteome changes in the zebrafish muscle caused by low doses of X-rays exposure.
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