ESS - APCT - Posters apresentados em eventos científicos
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- Avaliação do método histoquímico GASMoC para demonstração de micobactériasPublication . Cunha, Rute; Silva, Susana; Silva, Regina A.O método histoquímico Ziehl-Neelsen (ZN) é o método eleito em Anatomia Patológica e Microbiologia para a demonstração de micobactérias em tecidos e amostras citológicas. É usada uma solução corante de carbol-fuscina que contém fenol, um composto muito corrosivo e tóxico, que provoca efeitos adversos quando em exposição crónica. Recentemente, foi desenvolvido um método alternativo e isento de fenol (GASMoC), testado apenas em amostras de tecido infetado por duas espécies de Mycobacterium.
- CINtec PLUS cytology as a cervical cancer screening test at the IPO of PortoPublication . Alves, Ana; Lopes, Ana Paula; Granja, Sara; Quintela Vieira, Filipa; Silva, Regina AugustaCervical squamous cell carcinoma of the cervix is the fourth most common cancer between women and seventh globally. There are many women undergoing colposcopy without real need, so more effective screening tests are needed to decide who among HPV-positive women should receive additional diagnostic evaluation to avoid unnecessary colposcopies. So, this study is intended to evaluate CINtec PLUS cytology as a screening test for cervical cancer in the IPO-FG of the Port of women with hpv test positive other than 16/18, improving the referral for colposcopy. CINtec PLUS cytology is an immunocytochemistry kit that simultaneously detects p16 and Ki-67 proteins. The presence of these proteins in the same cell indicates a deregulation of the cell cycle, and may be a marker of the persistence of HPV infections, their greater probability of progression and the severity of lesions secondary to the infection. An experimental research was carried out, in which the immunostaining of 73 non-16/18 HPV cervico-vaginal hpv samples was performed from 73 women integrated in cervical cancer screening in the North region carried out at the IPO-FG of Porto. The results obtained in CINtec PLUS were compared with those of cytology. 2 samples present unsatisfactory cytology for evaluation, being excluded. Cintec Plus cytology would reduce by 17% the number of women sent for colposcopy (7 (about 10%) by Cintec Plus vs 19 (about 27%) by cytology). Of the 52 NILM samples, 3 (about 6%) were positive for Cintec Plus, so they should have been referred for colposcopy and were not. Of the 10 ASC-US samples 100% are negative for Cintec Plus, as well as 5 LSIL 4 (80%) are negative, of the 3 HSIL 2 (about 67%) are negative. So, 16 women (about 23%) made colposcopy unnecessarily. In conclusion, Cintec Plus cytology would be an interesting tool as a second screening test in non-16/18 hpv cases in place of cytology, decreasing the number of women sent for colposcopy.
- Congenital heart defects in isolated and syndromic contextPublication . Silva, Cátia; Varela, Catarina; Almeida, Cláudia; Gomes, Marcos; Brochado, Paulo; Nogueira, RoseteCongenital heart defects (CHD) is defined as a coarse structural abnormality of the heart or intrathoracic large vessels and which has functional significance. The objectives of this study were to determine the percentage of CHD in fetal autopsies and its frequency in isolated and syndromic context evaluate the most frequent types of CHD and determine the impact of histological study in the diagnosis of cardiac structural abnormalities.
- Cuidar ao cuidar - Estratégias e ferramentas para promover a saúde mental e emocional dos cuidadores.Publication . Fernandes, Sílvia; Portugal, Paula; Silva, Regina A.O Family Caregiver Support - Strategies and Tools to Promote Caregivers’ Mental and Emotional Health é um projeto financiado pelo Programa Erasmus+ ação-chave 2-parcerias estratégicas para educação de adultos, e conta com a colaboração de vários países da União Europeia como parceiros-membro. A necessidade deste projeto surge do levantamento de necessidades efetuado a nível europeu, que revela que há uma sobrecarga muito grande dos cuidadores familiares de indivíduos com doença mental grave: estes gastam em média 6 a 9 horas por dia na prestação de cuidados e, em mais de um terço dos casos, são os únicos cuidadores desse paciente. Apesar do seu papel primordial, os cuidadores sentem o seu trabalho desvalorizado pelos sistemas de saúde e políticos, para além de colocarem em risco a sua saúde física, mental e emocional. Este projeto surge com o objetivo de capacitar os cuidadores informais para desenvolverem autoconfiança e competências no desempenho das suas funções. Para o efeito, irão ser desenvolvidos um conjunto de recursos on-line contemplando conteúdos relevantes para a formação e informação no âmbito da literacia em saúde mental e de ferramentas úteis que os capacitem a cuidar de si ao cuidar dos outros. Estes recursos irão ser disponibilizados de uma forma interativa numa plataforma online e através de uma aplicação móvel. Desta forma, este projeto assenta num pilar base para a educação de adultos no âmbito da promoção da saúde, física, mental e emocional do cuidador.
- CytoPath®Easy: screening of cervical cancerPublication . Fernandes, Sílvia; Vilarinho, Ana Sofia; Silva, ReginaRecently, CytoPath®Easy kit was created by DiaPath S.p.A. and started to be commercially available for the screening of cervical cancer. Using this methodology, epithelial cells are immersed in a preservative liquid, and a thin-layer of cells in the slide is obtained through gravity sedimentation and filtration. The main objectives of this study are to evaluate the efficacy for the processing of cervical samples, for the detection of pre-neoplastic lesions and for the nucleic preservation and extraction by the kit. For this purpose, 215 cervical samples obtained by self-sampling were used: 174 were collected and processed by CytoPath® Easy and, as a control, 41 were collected and processed by the Thinprep® method. The samples were processed, stained by the Papanicolaou method, and independently evaluated microscopically for various morphological parameters; nucleic acids were isolated and evaluated for purity and integrity by spectrophotometry. Results obtained showed that both methods have a good performance, allowing the morphological evaluation of the cervical epithelium. However, the statistical analysis reveals that the methods are different from each other, with overall lower results being obtained in the method under study (p<0.001). In turn, both methods allow the extraction of good quality and quantity of DNA. Although some differences were found regarding morphology of the cells fixed and processed by the CytoPath®Easy method, this new methodology reveals efficient for the preservation of nucleic acids. Thus, its use in cervical cancer screening is recommended.
- Effects of different storage conditions of sequencing products with formamide in the quality of sequences.Publication . Moura, Daniela; Pina, Maria João; Pereira, Cátia; Baixia, Márcia; Leal, Rafaela; Sousa, Sónia; Vieira, Filipa Quintela; Silva, Regina; Cirnes, LuísDNA sequencing is widely used in molecular diagnosis and good sequence quality is crucial to a correct interpretation. It has been described that formamide quality decrease and sequencing reactions exposure to light, heat and/or oxygen can cause irregular peaks of cytosine and guanine in electropherograms. In a previous study, we concluded that despite the presence of this artifact when formamide is stored under non-ideal conditions, it does not significantly reduce the quality of the sequences.
- Environmental epigenetics and Daphnia as a model organismPublication . Pereira, Ana Rita; Silva, Regina; Barros, PiedadeDaphnia’s cyclic parthenogenesis life cycle is controlled by environmental cues and determines the population genetic variability. The asexual reproduction maintains the genetic background. This, allied to the fact that epigenetic changes were already described in Daphnia turns it in a useful model to study the effect of epigenetic changes in the organism.
- Epidemiologia do cancro do colo uterino e o impacto da vacinação contra o HPV: a realidade portuguesa no contexto europeuPublication . Marques, Ana; Fernandes, Sílvia; Silva, Regina A.A atual disponibilidade de vacinas e testes de rastreio molecular para o HPV aumentaram a possibilidade de eliminar globalmente o cancro cervical e outras patologias relacionadas com o vírus. No entanto, têm surgido vários obstáculos à implementação dos programas de vacinação contra o HPV a nível mundial. Este estudo tem como objetivos compilar e analisar estatisticamente a informação acerca da vacinação contra o HPV e políticas de rastreio de cancro cervical, em Portugal e em outros países europeus; e avaliar o impacto na incidência e mortalidade de cancro cervical, ao longo dos anos.
- Impact of different storage conditions of formamide in the quality of sequencesPublication . Moura, Daniela; Pina, Maria João; Pereira, Cátia; Baixia, Márcia; Leal, Rafaela; Sousa, Sónia; Vieira, Filipa Quintela; Silva, Regina; Cirnes, LuísObtaining high quality sequences is essential to proper reading and interpretation. A crucial step for this goal is the denaturation of sequencing products which is usually performed using a denaturing agent such as formamide. It has been described that the reduction of formamide quality may cause irregular cytosine and guanine peaks in an electropherogram.According to manufacturers recomendations, formamide should be stored at -20ºC. Under other conditions such as exposition to light and air and temperature changes for a certain period of time, quality may decrease. Objectives: The purpose of this study was to understand which formamide storage-related variables can be responsible for decreasing sequence quality.
- Molecular profiling of prognostic biomarkers in MelanomaPublication . Jesus, Marta; Correia, Beatriz; Costa, Catarina; João, Alexandre; Lopes, José Manuel; Barata, Catarina; Soares, Paula; Pópulo, HelenaCutaneous melanoma (CM) is the most aggressive and deadliest form of skin cancer (1). Its development is influenced by the genetic background, genetic mutations and environmental factors. The three most frequent mutations of CM appear in BRAF gene, which leads to uncontrolled cell growth, in NRAS gene, that disrupts normal cell signaling, and in TERT promoter, that increases telomerase activity and play a crucial role in tumor progression, influencing melanoma aggressiveness and patient outcome (2, 3). Despite advances in treatment, the prognosis of patients with advanced-stage remains poor (4). This work aims to improve prognosis determination by correlating identified molecular alterations with clinicopathological data. The analysis is being conducted on primary tumors collected at Hospital dos Capuchos. The study currently includes 29 samples collected between 2023 and 2024, 18 from male subjects and 11 from female subjects, with an average age of 69. Most of the melanomas were located on the trunk, and the most common diagnoses were nodular melanoma and superficial spreading melanoma. The mean Breslow thickness of the series is 3.78 mm, and 46% of samples have a mitotic index greater than 1. The samples were subjected to histopathological evaluation, followed by PCR and sequencing techniques, to identify the three mutations. Subsequent statistical analysis will be performed to assess the correlation between these genetic changes and clinicopathological features, with a focus on determining their impact on patient outcomes. The work is still ongoing, but the results will provide further information about the three mutations and their corresponding aggressiveness, as well as how the coexistence of different mutations influences patient prognosis. This study underlines the significance of molecular profiling in the prognosis assessment of melanoma patients, and it will provide a foundation for further research on molecular-based prognosis models.