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ESS - APCT - Posters apresentados em eventos científicos

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  • Avaliação do método histoquímico GASMoC para demonstração de micobactérias
    Publication . Cunha, Rute; Silva, Susana; Silva, Regina A.
    O método histoquímico Ziehl-Neelsen (ZN) é o método eleito em Anatomia Patológica e Microbiologia para a demonstração de micobactérias em tecidos e amostras citológicas. É usada uma solução corante de carbol-fuscina que contém fenol, um composto muito corrosivo e tóxico, que provoca efeitos adversos quando em exposição crónica. Recentemente, foi desenvolvido um método alternativo e isento de fenol (GASMoC), testado apenas em amostras de tecido infetado por duas espécies de Mycobacterium.
    2023-03Documento de conferência Acesso aberto Ver mais
  • Blockingmethamphetamine-induced microglia reactivity by targeting glutamate receptors
    Publication . Summavielle, Teresa; Canedo, Teresa; Silva, Ana Isabel; Andrade, Elva Bonifácio; Almeida, Tiago O.; Bravo, Joana; Terceiro, Ana Filipa; Canedo, Teresa; Silva, Ana Isabel; Magalhães, Ana; Relvas, João B.; Bonifácio Andrade, Elva; Bravo, Joana
    Exposure to psychostimulants has been classically associated with damage to neuronal terminals. However, it is now accepted that interaction between neuronal and glial cells also contributes to the addictive behavior. We have recently shown that acute methamphetamine (Meth), a powerful psychostimulant, causes microgliosis and increases microglia activation through astrocytic-TNF release1. We are now interested in clarifying the progression of neuroinflammation under chronic drug exposure and how different brain and immune cells contribute to this inflammatory process.To explore this, firstly, we performed a proteomic analysis, in different phases of the addictive process, in mice exposed to an escalating dosing of Meth for ten days (Meth10d). To validate the conditioning power of our model, mice were tested in a condition place preference (CPP) at 10d of Meth, and 2 or 10 days of withdrawal (WD). At all these time points, mice were seen to be strongly conditioned by Meth. Next, we conducted a proteomic analysis to compare the different time points (using the hippocampus, where we previously found robust microgliosis underMeth1). We found a proteome profile that varied substantially with exposure (Meth10d) and after a short- (WD2d)and long-term withdrawal (WD10d) periods. Interestingly, the most altered pathways were neuro transmitter-related.However, we also identified significant differences in Wnt signaling, which was previously linked to regulation of microglia reactivity. As such, we evaluated the microglia profile after chronic Meth exposure and at withdrawal. In the hippocampus, the number of microglia cells was significantly increased at Meth10d and remained also increased at WD2d. Microglia presented a more ameboid-like shape at Meth10d, but its ramified morphology was recovered at WD2d. Importantly, our proteomic data also revealed that during Meth withdrawal, several microglial receptors were down regulated, suggesting that microglia was in a “primed” state. In addition, as the crosstalk between neurons and microglia seems to be relevant for the behavioral expression of Meth, we are dissecting the modulation of microgliaby neurons under Meth exposure, to evaluate neuroimmune regulatory ligand-receptor pairs that seem to impact on the neuron-microglia interaction. Of note, some these ligand-receptor pairs seem to be down regulated by chronic Meth and during abstinence, which may be associated with reduced neuronal ability to down regulate microglia reactivity, and lead to increased neuronal damage. We fore see that these receptors may prove to be interesting therapeutic targets for the treatment of addiction, and therefore we will manipulate them to confirm their value in reducing relapse rates and improve addiction treatments.
    2023-07Póster em conferência Acesso aberto Ver mais
  • Caraterização de tumores sólidos numa amostra pediátrica do Instituto Português de Oncologia do Porto Francisco Gentil, EPE
    Publication . Santos, Ana; Serra, Carla; Mota, Marlene; Sousa, Manuela; Mendes, Carlos
    Caracterizar uma amostra pediátrica com TS quanto à sua frequência, óbito, sexo e idade ao diagnóstico. Estudar o possível envolvimento da MO ao diagnóstico, recorrendo a dados qualitativos de mielogramas. Estudo observacional descritivo transversal, dos registos dos dados de pacientes pediátricos com TS (n=148), diagnosticados entre 2000-2008. O TS mais frequente foi o Neuroblastoma (NB=37,2%), e os com maior registo de óbitos foram Rabdomiossarcoma (RMS=30,4%) e Família Tumores Ewing (FTE=28,0%). Nas raparigas os Tumor Wilms (TW=68,0%) e Osteossarcoma (58,3%) foram mais frequentes, enquanto nos rapazes foram os Retinoblastoma (75,0%), RMS (65,2%) e NB (61,8%). O NB manifestou-se, em 30,9% dos casos, até aos 12 meses de idade, o TW, em 32,0% dos casos, até aos 2 anos, e o RMS, em 69,6% dos casos, entre os 1-10 anos. A maioria dos pacientes com FTE (80,0%), NB (74,6%) e RMS (73,9%) realizaram mielograma ao diagnóstico. Observando os resultados qualitativos do mielograma, os tumores onde poderá ter ocorrido invasão da MO foram o NB e RMS. Na frequência dos TS verificou-se que seguem o descrito na literatura, excepto a FTE que surge em segundo lugar conjuntamente com o TW, sendo o NB mais frequente. Nos óbitos, o tumor com maior registo, RMS, não corresponde ao com maior gravidade (NB) descrito na investigação. Para estes resultados pode ter contribuído o número reduzido da amostra. Relativamente ao sexo, o NB e o RMS são mais frequentes nos rapazes, enquanto o TW é mais frequente nas raparigas, como descrito na literatura. Doentes com NB, TW e RMS apresentaram idades ao diagnóstico que indicam um melhor prognóstico. O NB, RMS e FTE, em alguns casos, disseminam-se para a MO, podendo ser esta a explicação para os resultados obtidos quanto à realização ou não de mielograma. Dos resultados obtidos nos mielogramas, verifica-se que poderá ter ocorrido invasão medular nos NB e RMS. Propõe-se continuar este estudo com amostra, por TS, mais representativa e com dados dos hemogramas e biópsias ósseas.
    2010-07-14Póster em conferência Acesso aberto Ver mais
  • CINtec PLUS cytology as a cervical cancer screening test at the IPO of Porto
    Publication . Alves, Ana; Lopes, Ana Paula; Granja, Sara; Quintela Vieira, Filipa; Silva, Regina Augusta
    Cervical squamous cell carcinoma of the cervix is the fourth most common cancer between women and seventh globally. There are many women undergoing colposcopy without real need, so more effective screening tests are needed to decide who among HPV-positive women should receive additional diagnostic evaluation to avoid unnecessary colposcopies. So, this study is intended to evaluate CINtec PLUS cytology as a screening test for cervical cancer in the IPO-FG of the Port of women with hpv test positive other than 16/18, improving the referral for colposcopy. CINtec PLUS cytology is an immunocytochemistry kit that simultaneously detects p16 and Ki-67 proteins. The presence of these proteins in the same cell indicates a deregulation of the cell cycle, and may be a marker of the persistence of HPV infections, their greater probability of progression and the severity of lesions secondary to the infection. An experimental research was carried out, in which the immunostaining of 73 non-16/18 HPV cervico-vaginal hpv samples was performed from 73 women integrated in cervical cancer screening in the North region carried out at the IPO-FG of Porto. The results obtained in CINtec PLUS were compared with those of cytology. 2 samples present unsatisfactory cytology for evaluation, being excluded. Cintec Plus cytology would reduce by 17% the number of women sent for colposcopy (7 (about 10%) by Cintec Plus vs 19 (about 27%) by cytology). Of the 52 NILM samples, 3 (about 6%) were positive for Cintec Plus, so they should have been referred for colposcopy and were not. Of the 10 ASC-US samples 100% are negative for Cintec Plus, as well as 5 LSIL 4 (80%) are negative, of the 3 HSIL 2 (about 67%) are negative. So, 16 women (about 23%) made colposcopy unnecessarily. In conclusion, Cintec Plus cytology would be an interesting tool as a second screening test in non-16/18 hpv cases in place of cytology, decreasing the number of women sent for colposcopy.
    2022-10-21Documento de conferência Acesso aberto Ver mais
  • Computacional pathology: What’s new
    Publication . Coelho, Daniel; Assunção, Teresa; Borrecho, Gonçalo; Geraldes, Mariana; Vinagre, Tiago; Ferreira, Inês; Ferreira, Ana; Fernandes, Ana Isabel; França, Amélia; Vale, João; Curado, Mónica; Mendes, Fernando; Martins, Diana
    The term computacional pathology (CPath) has become a buzz-word among the digital pathology community. Adances in scanning systems, imaging technologies and storage devices are generating an ever-increasing volume of whole-slide images (WSI) acquired in clinical settings, which can be computacionally analyzed using artificial intelligence (AI), such as deep learning technologies, in a new área of development called CPath. The purpose of the review is to disseminate the latest news and futures perspectives by CPath. Deep learning in the context of CPath has methodological contributions that can be distinguished into approaches based on the final purpose of the analysis: predicting clinical endpoints such as cancer subtype, patient survival or genetic mutations from WSI and AI-based assistive tools, such as segmentation methods for WSI or virtual staining. The emergence of multipex imaging, spatially resolver genomic assays and 3D pathology, among other methodologies, will accelerate this trend, providing new opportunities for multimodal integration and discovering new biomarkers. Additionally, these developments will help automating labor-intensive manual work and reducing inter-observer variability diagnosis between pathologists, contributing to a better patient care. CPath will underpin the development of the next generation of cancer therapies and diagnostics, changing the clinical research and ultimately leading towards new cures or improved patient outcomes.
    2024-03Póster em conferência Acesso aberto Ver mais
  • Congenital heart defects in isolated and syndromic context
    Publication . Silva, Cátia; Varela, Catarina; Almeida, Cláudia; Gomes, Marcos; Brochado, Paulo; Nogueira, Rosete
    Congenital heart defects (CHD) is defined as a coarse structural abnormality of the heart or intrathoracic large vessels and which has functional significance. The objectives of this study were to determine the percentage of CHD in fetal autopsies and its frequency in isolated and syndromic context evaluate the most frequent types of CHD and determine the impact of histological study in the diagnosis of cardiac structural abnormalities.
    2015-11Documento de conferência Acesso aberto Ver mais
  • Cuidar ao cuidar - Estratégias e ferramentas para promover a saúde mental e emocional dos cuidadores.
    Publication . Fernandes, Sílvia; Portugal, Paula; Silva, Regina A.
    O Family Caregiver Support - Strategies and Tools to Promote Caregivers’ Mental and Emotional Health é um projeto financiado pelo Programa Erasmus+ ação-chave 2-parcerias estratégicas para educação de adultos, e conta com a colaboração de vários países da União Europeia como parceiros-membro. A necessidade deste projeto surge do levantamento de necessidades efetuado a nível europeu, que revela que há uma sobrecarga muito grande dos cuidadores familiares de indivíduos com doença mental grave: estes gastam em média 6 a 9 horas por dia na prestação de cuidados e, em mais de um terço dos casos, são os únicos cuidadores desse paciente. Apesar do seu papel primordial, os cuidadores sentem o seu trabalho desvalorizado pelos sistemas de saúde e políticos, para além de colocarem em risco a sua saúde física, mental e emocional. Este projeto surge com o objetivo de capacitar os cuidadores informais para desenvolverem autoconfiança e competências no desempenho das suas funções. Para o efeito, irão ser desenvolvidos um conjunto de recursos on-line contemplando conteúdos relevantes para a formação e informação no âmbito da literacia em saúde mental e de ferramentas úteis que os capacitem a cuidar de si ao cuidar dos outros. Estes recursos irão ser disponibilizados de uma forma interativa numa plataforma online e através de uma aplicação móvel. Desta forma, este projeto assenta num pilar base para a educação de adultos no âmbito da promoção da saúde, física, mental e emocional do cuidador.
    2019-05Documento de conferência Acesso aberto Ver mais
  • CytoPath®Easy: screening of cervical cancer
    Publication . Fernandes, Sílvia; Vilarinho, Ana Sofia; Silva, Regina
    Recently, CytoPath®Easy kit was created by DiaPath S.p.A. and started to be commercially available for the screening of cervical cancer. Using this methodology, epithelial cells are immersed in a preservative liquid, and a thin-layer of cells in the slide is obtained through gravity sedimentation and filtration. The main objectives of this study are to evaluate the efficacy for the processing of cervical samples, for the detection of pre-neoplastic lesions and for the nucleic preservation and extraction by the kit. For this purpose, 215 cervical samples obtained by self-sampling were used: 174 were collected and processed by CytoPath® Easy and, as a control, 41 were collected and processed by the Thinprep® method. The samples were processed, stained by the Papanicolaou method, and independently evaluated microscopically for various morphological parameters; nucleic acids were isolated and evaluated for purity and integrity by spectrophotometry. Results obtained showed that both methods have a good performance, allowing the morphological evaluation of the cervical epithelium. However, the statistical analysis reveals that the methods are different from each other, with overall lower results being obtained in the method under study (p<0.001). In turn, both methods allow the extraction of good quality and quantity of DNA. Although some differences were found regarding morphology of the cells fixed and processed by the CytoPath®Easy method, this new methodology reveals efficient for the preservation of nucleic acids. Thus, its use in cervical cancer screening is recommended.
    2022-10-21Documento de conferência Acesso aberto Ver mais
  • Effects of different storage conditions of sequencing products with formamide in the quality of sequences.
    Publication . Moura, Daniela; Pina, Maria João; Pereira, Cátia; Baixia, Márcia; Leal, Rafaela; Sousa, Sónia; Vieira, Filipa Quintela; Silva, Regina; Cirnes, Luís
    DNA sequencing is widely used in molecular diagnosis and good sequence quality is crucial to a correct interpretation. It has been described that formamide quality decrease and sequencing reactions exposure to light, heat and/or oxygen can cause irregular peaks of cytosine and guanine in electropherograms. In a previous study, we concluded that despite the presence of this artifact when formamide is stored under non-ideal conditions, it does not significantly reduce the quality of the sequences.
    2015-11Documento de conferência Acesso aberto Ver mais
  • Environmental epigenetics and Daphnia as a model organism
    Publication . Pereira, Ana Rita; Silva, Regina; Barros, Piedade
    Daphnia’s cyclic parthenogenesis life cycle is controlled by environmental cues and determines the population genetic variability. The asexual reproduction maintains the genetic background. This, allied to the fact that epigenetic changes were already described in Daphnia turns it in a useful model to study the effect of epigenetic changes in the organism.
    2015-11Documento de conferência Acesso aberto Ver mais