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DNA sequencing is widely used in molecular diagnosis and good sequence quality is crucial to a correct interpretation. It has been described that formamide quality decrease and sequencing reactions exposure to light, heat and/or oxygen can cause irregular peaks of cytosine and guanine in electropherograms. In a previous study, we concluded that despite the presence of this artifact when formamide is stored under non-ideal conditions, it does not significantly reduce the quality of the sequences.
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Moura, D., Pina, M. J., Pereira, C., Baixia, M., Leal, R., Sousa, S., Vieira, F. Q., Silva, R. A., & Cirnes, L. (2015). Effects of different storage conditions of sequencing products with formamide in the quality of sequences. In R. A. Silva, P. Baylina, F. Q. Vieira, C. Barrias, M. Rubim, T. Coelho, C. Prudêncio, & Â. Jesus (Eds.), II Congresso Internacional da Saúde Gaia Porto: Livro de Resumos (1st ed., Vol. 1, pp. 123). Escola Superior de Tecnologia da Saúde do Instituto Politécnico do Porto.
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Escola Superior de Tecnologia da Saúde do Instituto Politécnico do Porto