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- Physical exercise and it influence in salivary cortisol levelsPublication . Trancoso, Ana; Ferreira, Stephanie; Moreira, Anabela; Moreira, Teresa; Mota, Sandra; Amorim, ManuelaCortisol is important for the individual survival since it interferes with several of the body's systems. Only free cortisol fraction can be determined in saliva. Although saliva presents advantages compared to other biological products for the cortisol measurement, it is influenced by several factors - physical and psychological stress.
- Microbiological characterization of thermal watersPublication . Machado, Adriana; Amorim, Manuela; Ferreira, Stephanie; Condeço, Jorge; Mota, Sandra; Moreira, Anabela; Augusto, AntónioThermal waters are used for disease prevention or treatment, for rehabilitation or welfare. The health risks associated with the use of these waters depends not only on the nature of the waters, but also on the manner of its use and the immune status of users. Exposure to microorganism scan occur through inhalation, ingestion or contact. The related severity and diversity of diseases leads to the verification of bacteriological standards established by law in order to ensure its quality and safety.
- Microbiological quality control of minced beef and porkPublication . Alves, Sílvia; Moreira, Anabela; Ferreira, Stephanie; Moreira, Teresa; Mota, Sandra; Araújo, António; Amorim, ManuelaFood-borne infections are responsible for high rates of mortality and morbidity. To evaluate and compare the results of microbiological analysis of minced beef and pork.
- Microbiological evaluation of vegetable salads in school canteensPublication . Carneiro, Mariana; Amorim, Manuela; Ferreira, Stephanie; Mota, Sandra; Moreira, Anabela; Augusto, AntónioCurrently bacteria are the main responsible for infections and food poisoning, being a huge public health problem worldwide. Vegetables are potential vehicles of these microorganisms and their consumption, especially raw, increases contamination risk. It has been reported an increase in diseases associated with microbiological contamination by vegetables consumption. Vegetable cultivation and growth process makes them vulnerable to contamination sources that may act at any stage from planting to consumption. Salmonella, Escherichiacoli , Staphylococcus and Listeria are the microorganisms most commonly isolated from vegetables responsible for food borne diseases.
- Nucleic acids extraction equipment comparisonPublication . Marques, Ana; Monteiro, Fátima; Moreira, Anabela; Ferreira, Stephanie; Moreira, Teresa; Mota, Sandra; Amorim, ManuelaThe nucleic acids extraction is the first step towards molecular diagnostic tests. It is a process susceptible to various interferences that can cause target material shortage. The objective of this study is to compare the methodological and operational characteristics of nucleic acids extraction equipment MagnaPure LC and QIAsymphony SP.
- Microbiological characterization of haemodialysis waters in a dialysis centre at Northern PortugalPublication . Azevedo, Joana; Amorim, Manuela; Ferreira, Stephanie; Mota, Sandra; Moreira, Anabela; Augusto, AntónioThe main therapeutics for chronic renal failure are haemodialysis, peritoneal dialysis and renal transplantation, which seek to replace renal function (Coulliette & Arduino, 2013). The high-flux haemodialysis is a sequential process, constituted of water pre-treatment (Entry-EA, reverse osmosis-AROI, after osmosis 1-DROI, after osmosis and ring return –RAD), and water treatment in patient system (Damasiewicz, Polkinghorne, & Kerr, 2012). To ensure treatment effectiveness, it is necessary microbiological levels within acceptable limits, which depend on optimal operating conditions in central distribution of dialysis and water quality used for dialysate (Damasiewicz et al., 2012; Ministério da Sáude, 2001). This requires water microbiological and chemical parameters analysis and an effective monitoring to verify that it is suitable for high-flux hemodialysis (Damasiewicz et al., 2012; Ministério da Sáude, 2001).
- Swimming pool water quality in the northern zone of PortugalPublication . Ferreira, Stephanie; Amorim, Manuela; Araújo, António; Mota, SandraThe use of swimming pools for recreational purposes is associated with several benefits for the health and wellbeing of its users, however it is necessary to monitor the quality of the water in terms of public health. With the aim of evaluating the microbiological quality of swimming pool water, the results of 1280 water samples from swimming pools in the North of Portugal were analyzed. The evaluation of the water quality was carried out through the research and quantification of microorganisms viable at 37ºC, Coliform Bacteria, Enterococci, Escherichia coli, Pseudomonas aeruginosa, Coagulase Positive Staphylococci and the Total Number of Staphylococci.
- Adaptação de um instrumento para avaliar a satisfação na área da saúde mentalPublication . Mota, Sandra; Moreira, Anabela; Ferreira, Stephanie; Amorim, ManuelaA avaliação da satisfação dos utentes de instituições de saúde tem sido uma área de interesse crescente. Contudo, nas instituições de saúde mental, este interesse é mais recente, refletindo a necessidade destas instituições também demonstrarem a qualidade dos seus serviços. Tendo como objetivo adaptar um instrumento para avaliar a satisfação dos utentes de internamento na área da saúde mental, procedeu-se à adaptação cultural e linguística de um instrumento já validado em Itália “The Rome Opinion Questionnaire for Psychiatric Wards”. Procedeu-se à sua tradução e retro tradução do instrumento original, comparação entre as duas versões na língua original, deteção, identificação e correção de possíveis discrepâncias entre as duas versões, concluindo-se assim a equivalência linguística e semântica do instrumento, resultando deste processo a primeira versão do instrumento. Dos resultados obtidos através da opinião e sugestões expressas pelos elementos do painel de peritos, destacaram-se alterações ao enunciado de vários itens, contudo este painel considerou que todos os itens eram relevantes e importantes para o estudo da satisfação e que as opções de resposta estavam devidamente ordenadas e coerentes com o que desejavam exprimir. Das alterações realizadas resultou a segunda versão do instrumento. Com este trabalho ficou concluída a adaptação cultural e linguística de instrumento de avaliação da satisfação dos utentes na área da saúde mental.
- Caracterização das infeções osteoarticulares associadas a prótese num hospital central, entre 2016 e 2022Publication . Silva, Paulo; Ferreira, Stéphanie; Amorim, ManuelaA infeção protésica é das complicações mais temíveis no decorrer de uma artroplastia e, dada a sua natureza multidisciplinar, a abordagem clínica permanece um desafio. Assim, é fundamental a cooperação entre especialidades médicas e cirúrgicas, destacando que um diagnóstico microbiológico o mais preciso possível é crucial para um tratamento efetivo (McNally M et al., 2021).
- Microbiological monitoring of surfaces and food handlersPublication . Barros, Sílvia; Amorim, Manuela; Ferreira, Stephanie; Condeço, Jorge; Mota, Sandra; Moreira, Anabela; Augusto, AntónioEvery year, foodborne diseases affects about 48 million people in the United States alone, usually associated with microbiological infection or intoxication The increase in demand for catering services has contributed to cases increase (Kleter & Marvin, 2009; Lund, A.C. Baird-Parker, 2000; Newell et al., 2010). These data show the importance of quality assurance from food production to the consumption. Therefore, it is necessary to take into account special care towards the environment (air), facilities, equipment, surfaces, utensils and handlers throughout the processing by cleaning processes and disinfection (Kleter & Marvin, 2009).