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- Principal component analysis of gait Continuous Relative Phase (CRP): Uncovering lower limb coordination biomarkers for functional disability in older adultsPublication . Moreira, Juliana; Alves, Leonel A. T.; Sousa, Rúben Oliveira; Castro, Márcia; Santos, Rubim; Sousa, Andreia S. P.; Pinheiro de Sousa, Andreia Sofia; Rubim Silva Santos, Manuel; Santos Moreira, JulianaSymmetry in gait coordination reflects the balanced timing and movement between lower limb joints, which are essential for efficient locomotion and functional independence in older adults. Although gait coordination is recognized as a key indicator of aging-related adaptations and functional decline, most studies rely on isolated measures without fully addressing symmetry in intra- and interlimb coordination. This study aimed to identify principal components of gait coordination symmetry and their association with functional disability in older adults. A cross-sectional study assessed 60 community-dwelling older adults (60+), stratified by functional disability (35 non-disabled; 25 disabled). The threedimensional range of motion of lower limb joints was assessed during the gait cycle using an optoelectronic system. Intra- and intersegmental coordination was assessed by the continuous relative phase (CRP), a nonlinear measure that captures both timing and movement relationships between joint angles. Principal component analysis was applied to CRP means and coefficients-of-variation (CV) to identify key coordination principal components (PC). Of eight PC explaining 78.86% of variance, only the PC1 distinguished disability status (p = 0.007, d = 0.66). This component included sagittal-plane intrasegmental CRP mean and CV for the knee–ankle and hip–ankle. This study is novel in combining CRP-derived measures of intra- and interlimb symmetry with principal component analysis to distinguish functional disability in older adults. The findings indicate that sagittal-plane intrasegmental CRP symmetry may serve a relevant biomarker of gait impairment. By linking kinematic coordination features to functional disability, this approach complements clinical assessments and supports early identification of mobility decline in older adults.
- Task demand effects on Visual and Auditory MMN across autistic and schizotypal traitsPublication . Mazer, Prune; Pasion, Rita; Rao, Zohra Kamran; Silveira, Celeste; Santos, Fernando Ferreira; Mazer, PruneMismatch negativity (MMN) is an event-related potential component automatically elicited by violations of sensory predictions and is widely interpreted, within the Predictive Processing framework, as a neural correlate of prediction error. Disruptions in prediction error signaling have been proposed as a potential mechanism underlying the diverse cognitive and perceptual profiles observed in autism and schizophrenia spectrum conditions. In this study, 122 community participants completed auditory and visual oddball tasks with two levels of target detection difficulty while undergoing EEG recording, alongside self-report measures of autistic and schizotypal traits. We found that increased task difficulty significantly reduced MMN amplitude in both modalities, with large effect sizes for auditory (d = 1.826) and visual (d = 1.005) MMN, indicating robust modulation by perceptual load. Although associations between MMN amplitude and trait dimensions were limited, emerging patterns suggest a potential dissociation between social and non-social autistic traits. These findings address key gaps in the literature, particularly the underrepresentation of visual MMN, and highlight the importance of multidimensional, cross-modal approaches to investigating prediction error mechanisms in neurodiverse populations.
- A comprehensive analysis of viability assays for Giardia lamblia and Trichomonas vaginalis trophozoites: a systematic reviewPublication . Sequeira, Susie; Sousa, Mariana; Cruz, AgostinhoGiardia lamblia and Trichomonas vaginalis are flagellated protozoan parasites that often cause asymptomatic infections but may lead to gastrointestinal or genitourinary symptoms. Improved treatment options are needed due to emerging resistance. However, selecting an appropriate method for assessing the in vitro susceptibility of G. lamblia and T. vaginalis in the presence of potential therapeutic compounds remains challenging due to the variability in these methods. This study aimed to provide an overview of commonly employed methods for determining trophozoite viability in the presence of potential therapeutic compounds and to propose a standardized viability assay for susceptibility testing for G. lamblia and T. vaginalis. A systematic literature review was conducted according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) 2020 statement, using databases including MEDLINE, ScienceDirect, and Web of Science, with the following search equation: “in vitro” AND “method” AND (“susceptibility” OR “viability” OR “sensitivity”) AND (“giardia” OR “trichomonas”). The search identified 32 experimental studies with diverse viability assays. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, adherence inhibition assay, and [3H]-thymidine incorporation assay were prominent for G. lamblia. The trypan blue assay, motility assessment, and resazurin assay were frequently used for T. vaginalis. These findings underscore the diversity in viability assessment methods, highlighting the importance of standardizing viability assays to ensure accurate and reproducible results in drug susceptibility studies. The fluorometric resazurin assay has emerged as a suitable choice for standardization in both parasites, offering cost-effectiveness, reliability, and ease of use.
- Comparative effectiveness of different probiotic delivery methods in oral Candidiasis: A Systematic ReviewPublication . Ashouritoustani, Reihaneh; Pinho, Cláudia; Oliveira, Ana Isabel; Barros, Piedade; Cruz, Agostinho; Cruz, Agostinho; Gonçalves de Barros, Piedade AuroraOral candidiasis, mainly from Candida albicans, affects immunocompromised individuals, the elderly, and denture wearers. Probiotics offer immunomodulatory and microbiota-balancing benefits as potential antifungal alternatives. However, the comparative impact of different probiotic delivery methods remains inadequately explored. This systematic review evaluated the effectiveness of various probiotic delivery methods in reducing Candida colonization and clinical symptoms in oral candidiasis. Following PRISMA 2020 guidelines, a systematic review search across multiple databases included human clinical studies based (Medline, Web of Science, ScienceDirect, and ProQuest) on PICO criteria across all age groups. Outcomes assessed included Candida load, oral microbiota changes, symptom improvement, and disease recurrence. Of 297 articles screened, 10 met inclusion criteria. Delivery methods investigated included lozenges, capsules, yogurt, and cheese. Most studies reported reductions in Candida colony-forming units (CFUs) or prevalence, mainly for C. albicans and for non-albicans species, with probiotics such as Lactobacillus reuteri, L. rhamnosus, L. acidophilus, and Bifidobacterium strains. Some studies reported improved immunological markers, while symptom relief, especially when probiotics were combined with antifungals. Probiotics reduce Candida colonization and symptoms, with potential prolonged effects. They show promise as adjunctive therapies, but standardized, large-scale trials are needed for optimization.
- DICER1 and DGCR8 in thyroid tumorigenesis: miRNA biogenesis and histopathologic diversityPublication . Rodrigues, Lia; Martins, Rui Sousa; Máximo, Valdemar; Soares, Paula; Vinagre, João; Nosé, Vânia; Canberk, Sule; Rodrigues, LiaThis review examines the emerging roles of DICER1 and DGCR8, key components of the miRNA biogenesis pathway, in thyroid pathogenesis, with a particular focus on their association with oncocytic morphology. Recent findings have expanded our understanding of DICER1 syndrome and DGCR8-related thyroid disorders, revealing a broader spectrum of thyroid lesions associated with mutations in these genes than previously recognised. We analyse the current literature on DICER1 and DGCR8 mutations in thyroid pathology, synthesising data from both basic science and pathological studies. The review explores recent findings on oncocytic features in some DICER1-mutated thyroid lesions, acknowledging that this association remains under investigation. The manuscript details the molecular mechanisms underlying DICER1 and DGCR8 mutations, including their impact on miRNA processing and subsequent effects on gene expression and cellular function. We discuss the diverse range of thyroid lesions associated with these mutations, from benign follicular nodular disease to aggressive carcinomas. The clinical implications of these findings are significant, as recognising DICER1 and DGCR8-related thyroid lesions can lead to improved patient management, including genetic counselling and surveillance for other associated malignancies. We propose an algorithm for identifying DICER1-related thyroid lesions, with a focus on oncocytic tumours, to aid clinicians and pathologists in recognising these entities. This emerging field promises to refine the diagnosis, management, and treatment of thyroid disorders associated with miRNA biogenesis pathway alterations, potentially leading to novel diagnostic and therapeutic approaches.
- Cross-cultural adaptation, validity and reliability of the European Portuguese Version of the Kerlan-Jobe Orthopaedic Clinic Shoulder & Elbow Score (KJOC)Publication . Almeida, Gonçalo; Amaral, Luísa; Vilarinho, Rui; Magalhães, Bárbara; Silva, Fátima; Abreu, Verónica; Magalhães, André; Esteves, Mário; Cervaens, Mariana; Vilarinho, RuiThe Kerlan-Jobe Orthopaedic Clinic Shoulder & Elbow Score (KJOC) is used to identify dysfunctions and estimate injury risk in overhead sports athletes. Although it has been validated in several countries, a European Portuguese version is currently unavailable. This study aimed to translate, culturally adapt and assess psychometric properties (validity and reliability) of the European Portuguese KJOC (KJOC-PT). The KJOC-PT was translated and culturally adapted according to international guidelines. One hundred athletes were selected (median age 24 [IQR 17] years, 72% male) and divided into two groups: asymptomatic and symptomatic athletes. The convergent validity was assessed by correlating the KJOC-PT with the Disabilities of the Arm, Shoulder and Hand (DASH) and DASH-Sports. 31 athletes from the initial sample were considered to assess between-day reliability and agreement (Bland-Altman analysis). Floor and ceiling effects were also calculated. Sampling adequacy was assessed using the Kaiser Meyer-Olkin (KMO) test. Minor cultural and linguistic changes were made in the KJOC-PT. This version demonstrated excellent internal consistency (Cronbach’s α = 0.91 to 0.97) and moderate negative correlations for validity (KJOC-PT with DASH, rho = −0.595; with DASH-Sports, rho = −0.533, both p < 0.001). Good reliability (ICC2,1 = 0.77 to 0.89 [95%CI 0.36 to 0.96]), measurement error (SEM = 4.11 to 6.90; MDC = 11.39 to 19.13) and mean difference ranging from −0.08 ± 6.14 to 3 ± 9.17 were found. No floor effect (0%) and ceiling effects of 24.2% for the total sample (50% for asymptomatic and 5.1% for symptomatic athletes) were found. KJOC-PT is now available and is a valid and reliable instrument for use by athletes in overhead sports.
- Bibliometric analysis towards industrial-scale use of Marine Algae and Lichens as soil amendments and plant biofertilizers for sustainable agriculturePublication . Ouala, Oumaima; Essadki, Yasser; Oudra, Brahim; Khalloufi, Fatima El; Martins, Rosário; Martins, RosarioThe nutrient-rich composition of seaweeds and lichens makes them well-suited for agricultural applications. Their use as alternatives to synthetic fertilizers contributes to sustainable agricultural production, enabling farmers to adopt ecological practices while maintaining or increasing crop productivity. This review aims to highlight the status and trends of research, along with a literature analysis on the application of these biomasses in sustainable agriculture. A bibliometric analysis was performed based on two databases (Scopus and Web of Science) to overview the main research topics regarding the use of biomasses studied in agriculture, thus providing useful information for future research. The biochemical composition and agricultural applications of these biomasses have been highlighted. The analysis shows that these biomasses are rich of nutrient compounds, revealing their roles and mechanisms of action on the chemical, nutritional properties, and soil microbial activities and their effect on plant growth, using various extraction and application methods. It also highlighted the potential of seaweeds for protection against biotic and abiotic stresses. In light of all the data presented in this review, it is possible to stimulate farmers’ interest in using seaweeds and lichens as natural fertilizers, with a focus on sustainable and ecological agriculture mainly in developing countries.
- Colouring applications of microalgae and cyanobacteria photosynthetic pigments: Challenges for industrial and market acceptancePublication . Reimão, Mariana; Almeida, Liliana; Ramos, Cristiana; Eusébio, Nádia; Martins, Rosário; Silva, Manuela Vieira da; Vasconcelos, Vítor; Freitas, Marisa; Freitas, Marisa; Vieira da Silva, Manuela; Martins, Rosario; REIMÃO BORGES LOPES DA SILVA, MARIANAMicroalgae and cyanobacterial photosynthetic pigments possess immense potential for sustainable colouring applications in industries such as food, feed, cosmetics and textiles, primarily because of their vibrant colours, safety and eco-friendliness. These photosynthetic pigments align with the growing demand for eco-conscious products. However, substantial challenges persist at the industrial level, particularly in scaling up production and achieving acceptance in niche markets. This review highlights the critical roles of abiotic factors such as light quality, temperature, pH, nutrient availability and salinity in enhancing pigment production using conventional production methods. Additionally, issues related to the cost, purity and stability of the pigments must be addressed to facilitate broader acceptance. Looking forward, the focus must be on developing innovative cultivation techniques that optimise and define the abiotic conditions most favourable for maximum pigment production or even incorporate genetic engineering tools. Additionally, exploring untapped species from inhospitable environmental sources and integrating the residual biomass through biorefinery processes represent effective approaches for enhancing the sustainability of microalgae and cyanobacterial pigment production on an industrial scale. Overcoming these barriers utilising targeted research and industry collaboration as well as fostering market acceptance is essential for fully harnessing the potential of these natural pigments and driving the transition towards more sustainable industrial colouring practices.
- Sustainability in allied health education and practice: An exploratory survey of student perspectives, knowledge, and attitudesPublication . Carvalhais, Carlos; Ribeiro, Inês; Xavier, Ana; Saúde, Miguel; Alberto Alves Carvalhais, CarlosThe growing urgency of the climate crisis has heightened the importance of integrating sustainability into health education. Allied health professionals are well positioned to lead sustainable healthcare efforts, yet evidence suggests a persistent gap between student awareness and formal training. This study explored the perspectives, knowledge, and attitudes of Portuguese allied health students regarding sustainability. An online and anonymous cross-sectional survey was conducted among undergraduate and graduate students across multiple allied health disciplines. The questionnaire assessed general knowledge, perceptions of curricular integration, and attitudes toward sustainable clinical practice. A total of 247 (response rate of 8.23%) students participated, with the majority expressing high concern about climate change and strong support for environmentally responsible healthcare. However, the results revealed inconsistent awareness of healthcare’s environmental footprint and a limited exposure to structured sustainability education. Friedman tests indicated significant variability in students’ knowledge, perceived responsibility, and curricular experiences. Students identified priority themes for curricular inclusion—such as the environmental impact of the health system—and recognized their alignment with the UN Sustainable Development Goals (SDGs), particularly SDG 3 (Good Health) and SDG 13 (Climate Action). The findings highlight the need for the systematic, competency-based integration of sustainability into allied health curricula to support a climate-resilient and ecologically responsible future healthcare workforce.
- Vitamin K Epoxide Reductase Complex (VKORC1) Electrochemical Genosensors: Towards the Identification of 1639 G>A Genetic PolymorphismPublication . Barbosa, Tiago; Morais, Stephanie L.; Carvalho, Renato; Magalhães, Júlia M. C. S.; Domingues, Valentina F.; Delerue-Matos, Cristina; Ferreira-Fernandes, Hygor; Pinto, Giovanny R.; Santos, Marlene; Barroso, Maria Fátima; Santos, Marlene; Manuel de Caldevilla Carvalho, RenatoAnticoagulants, including warfarin, are often administered to patients who are exhibiting early symptoms of thromboembolic episodes or who have already experienced such episodes. However, warfarin has a limited therapeutic index and might cause bleeding and other clinical problems. Warfarin inhibits the vitamin K epoxide reductase complex subunit 1 (VKORC1), an enzyme essential for activating vitamin K, in the coagulation cascade. Genetic factors, such as polymorphisms, can change the natural function of VKORC1, causing variations in the medication reaction among individuals. Hence, before prescribing warfarin, the patient’s genetic profile should also be considered. In this study, an electrochemical genosensor capable of detecting the VKORC1 1639 G>A polymorphism was designed and optimized. This analytical approach detects the electric current obtained during the hybridization reaction between two 52 base pair complementary oligonucleotide sequences. Investigating public bioinformatic platforms, two DNA sequences with the A and G single-nucleotide variants were selected and designed. The experimental protocol of the genosensor implied the formation of a bilayer composed of a thiolate DNA and an alkanethiol immobilized onto gold electrodes, as well as the formation of a DNA duplex using a sandwich-format hybridization reaction through a fluorescein labelled DNA signalling probe and the enzymatic amplification of the electrochemical signal, detected by chronoamperometry. A detection limit of 20 pM and a linear range of 0.05–1.00 nM was obtained. A clear differentiation between A/A, G/A and G/G genotypes in biological samples was successfully identified by his novel device.