Browsing by Author "Vieira, Filipa Quintela"
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- Atividade citotóxica de extratos de Taraxacum hispanicum em linhas celulares de cancro da mamaPublication . Magalhães, Ângela; Vieira, Filipa Quintela; Pinho, Cláudia; Oliveira, Ana; Cruz, Agostinho; Carmen, Jerónimo; Silva, ReginaAs plantas do género Taraxacum têm sido utilizadas tradicionalmente em tratamentos por apresentarem propriedades diuréticas, antioxidantes, anti-inflamatórias, hepatoprotetoras e antitumorais. O cancro da mama é um dos cancros mais comuns em Portugal, particularmente na região norte do país. Cerca de 75-80% dos casos de cancro da mama invasivo expressam recetores de estrogénio (RE), tendo uma boa resposta a terapias antiestrogénica. Contudo, uma parte destes tumores tornam-se estrogénio resistentes (ER), sendo os tratamentos utilizados para este tipo de tumores, assim como para o cancro metastático e/ ou recorrente, pouco efetivos. Assim, é necessário pesquisar novas alternativas terapêuticas seguras e eficazes.
- Avaliação da citotoxicidade de compostos sintéticos em linhas celulares de cancro da próstataPublication . Magalhães, Ângela; Vieira, Filipa Quintela; Vieira, Mónica; Ferraz, Ricardo; Prudêncio, Cristina; Carmen, Jerónimo; Silva, ReginaO cancro da próstata (CaP) constitui uma das principais causas de morbilidade e mortalidade oncológica quando diagnosticado em estadios avançados da doença. As terapêuticas atualmente disponíveis apresentam limitado efeito curativo, levando à progressão para um fenótipo altamente agressivo resistente à castração. Assim, torna-se imperativo o desenvolvimento de novas de agentes anti-tumorais. Os líquidos iónicos são sais orgânicos com atividade anti-neoplásica tendo vindo a ser alvo de estudo na indústria farmacêutica. As quinoxalinas são compostos aromáticos heterocíclicos com várias aplicações na medicina devido ao seu potencial anti-tumoral e anti-microbiano.
- Citotoxicidade de extratos de T. hispanicum em linhas celulares de cancro da próstataPublication . Magalhães, Ângela; Vieira, Filipa Quintela; Pinho, Cláudia; Oliveira, Ana; Cruz, Agostinho; Carmen, Jerónimo; Silva, ReginaAs plantas do género Taraxacum têm sido utilizadas, tradicionalmente, para o tratamento de vários tipos de cancro. No entanto, estudos relativos à sua atividade anticancerígena são escassos e o potencial mecanismo de ação ainda está pouco esclarecido. Dados do RORENO (2010) revelam que o cancro da próstata é um dos mais diagnosticados em Portugal e que o tratamento é pouco efetivo em estádios avançados. A terapia mais utilizada para o cancro da próstata localmente avançado e metastizado é a terapia de privação androgénica. Contudo, muitos doentes que respondem inicialmente ao tratamento desenvolvem posteriormente a doença resistente à castração. Pelo exposto, é necessário pesquisar novas alternativas terapêuticas seguras e eficazes.
- Effects of different storage conditions of sequencing products with formamide in the quality of sequences.Publication . Moura, Daniela; Pina, Maria João; Pereira, Cátia; Baixia, Márcia; Leal, Rafaela; Sousa, Sónia; Vieira, Filipa Quintela; Silva, Regina; Cirnes, LuísDNA sequencing is widely used in molecular diagnosis and good sequence quality is crucial to a correct interpretation. It has been described that formamide quality decrease and sequencing reactions exposure to light, heat and/or oxygen can cause irregular peaks of cytosine and guanine in electropherograms. In a previous study, we concluded that despite the presence of this artifact when formamide is stored under non-ideal conditions, it does not significantly reduce the quality of the sequences.
- Estudo de novos compostos sintéticos para o tratamento do cancro da mamaPublication . Magalhães, Ângela; Vieira, Filipa Quintela; Vieira, Mónica; Ferraz, Ricardo; Prudêncio, Cristina; Jerónimo, Carmen; Silva, ReginaO cancro da mama (CaM) apesar dos avanços no tratamento e deteção precoce desta neoplasia, permanece como uma das principais causas de morte por cancro nas mulheres devido à progressão e disseminação sistémica. Cerca de 80% dos CaM invasivos são positivos para o recetor de estrogénios sendo candidatos à terapia de bloqueio. No entanto, 30- 40% das doentes desenvolvem recidiva, evoluindo para um fenótipo hormono-resistente. Nestes estadios, as opções terapêuticas apresentam uma reduzida eficácia. Assim, é importante a descoberta de novos agentes anti-neoplásicos. Os líquidos iónicos e as quinoxalinas são sais orgânicos com potencial anti-tumoral que têm vindo a ser alvo de estudo na indústria farmacêutica devido às suas propriedades medicinais e composição flexível no caso dos líquidos iónicos.
- Expression of EMT-related genes CAMK2N1 and WNT5A is increased in locally invasive and metastatic prostate cancerPublication . Carneiro, Isa; Vieira, Filipa Quintela; Lobo, João; Moreira-Barbosa, Catarina; Menezes, Francisco Duarte; Martins, Ana Teresa; Oliveira, Jorge; Silva, Regina; Jerónimo, Carmen; Henrique, RuiProstate cancer (PCa) varies clinically from very indolent, not requiring therapeutic intervention, to highly aggressive, entailing radical treatment. Currently, stratification of PCa aggressiveness is mostly based on Gleason score, serum PSA and TNM stage, but outcome prediction in an individual basis is suboptimal. Thus, perfecting pre-therapeutic discrimination between indolent and aggressive PCa, avoiding overtreatment is a major challenge. Epithelial to mesenchymal transition (EMT) allows epithelial cells to acquire mesenchymal properties, constituting a critical step in tumor invasion and metastization. Thus, we hypothesized that EMT-related markers might allow for improved assessment of PCa aggressiveness. Using RealTime ready Custom Panel 384 assay, 93 EMT-related genes were assessed in normal prostate tissues (NPT, n=5), stage pT2a+b-PCa (n=5) and stage pT3b-PCa (n=5), from which CAMK2N1, CD44, KRT14, TGFβ3 and WNT5A genes emerged as the most significantly altered. Expression levels were then evaluated in a larger series (16 NPT and 94 PCa) of frozen tissues using quantitative RT-PCR. Globally, CAMK2N1, CD44 and WNT5A displayed higher expression levels at higher stages and less differentiated PCa. CAMK2N1 and WNT5A immunoexpression analysis disclosed significantly lower expression in NPT and increasing proportion of high-expression cases from pT2a+b to pT3b and metastatic PCa. Furthermore, higher CAMK2N1 and WNT5A transcript levels associated with shorter disease-free and disease-specific survival. In multivariable analysis, a trend for WNT5A expression levels to independently predict DFS was disclosed (p=0.056). Globally, our findings suggest an association between PCa aggressiveness and increased expression of CAMK2N1 and WNT5A, reflecting the acquisition of effective EMT characteristics by PCa cells.
- Impact of different storage conditions of formamide in the quality of sequencesPublication . Moura, Daniela; Pina, Maria João; Pereira, Cátia; Baixia, Márcia; Leal, Rafaela; Sousa, Sónia; Vieira, Filipa Quintela; Silva, Regina; Cirnes, LuísObtaining high quality sequences is essential to proper reading and interpretation. A crucial step for this goal is the denaturation of sequencing products which is usually performed using a denaturing agent such as formamide. It has been described that the reduction of formamide quality may cause irregular cytosine and guanine peaks in an electropherogram.According to manufacturers recomendations, formamide should be stored at -20ºC. Under other conditions such as exposition to light and air and temperature changes for a certain period of time, quality may decrease. Objectives: The purpose of this study was to understand which formamide storage-related variables can be responsible for decreasing sequence quality.
- Morphofunctional characterization of the ileum in a type 1 diabetes rat model 7Publication . Henriques, Vanessa Mendes; Monteiro, Salomé Gonçalves; Vieira, Filipa Quintela; Sá, Paulo Correia de; Araújo, Margarida DuarteDiabetes mellitus (DM) is a metabolic disorder that affects 1 in 12 people with high mortality and morbidity. Knowing that about 76% of diabetic patients have gastrointestinal complications, we decided to characterize morphologically and functionally the ileum of a rat (Rattus norvegicus, Wistar) model that mimics type 1 DM. Diabetes was chemically induced by a single intraperitoneal injection of streptozotocin (STZ, 55mg/kg). All animals were used 14 days after injection with saline (CTRL-group) or STZ (STZ-group).
- Natural and synthetic compounds as anti-cancer therapies for breast and prostate cancerPublication . Magalhães, Ângela; Vieira, Filipa Quintela; Ferraz, Ricardo; Pinho, Cláudia; Vieira, Mónica; Oliveira, Ana; Prudêncio, Cristina; Cruz, Agostinho; Jerónimo, Carmen; Silva, ReginaBreast Cancer (BCa) and Prostate Cancer (PCa) are the most prevalent tumors in females and males, respectively, being the main causes of cancer morbidity and mortality, worldwide. Currently, there is no available curative treatment for the hormone-resistant tumors, being the development of new therapeutic strategies using innovative anticancer agents imperative. Thus, the main goal of this work was to evaluate the anticancer properties of natural and synthetic compounds through in vitro assays.
- Optimisation of a molecular methodology for the detection of virulence factors of enterotoxigenic Escherichia coli for the diagnosis of swine colibacillosisPublication . Campos, Ana; Oliveira, Ricardo; Almeida, Carina; Vieira, Filipa Quintela; Silva, Regina AugustaThe most common bacterial pathogen causing enteric infections in pigs is enterotoxigenic Escherichia coli (ETEC). Since pigs represent the largest livestock category in the European Union, ETEC-associated diseases, better known as swine colibacillosis leading to acute diarrhea and eventual death of the animal, result in significant costs to the pig industry. These diseases are traditionally prevented or treated with antibiotics, and this has had a huge impact on the emergence of resistant bacteria, correlating with the emergence of resistant infections in humans. Recognition of this problem has led the authorities to set ambitious goals for the reduction of this type of drug in animal husbandry, leading to the creation of a national project, APTAcoli, which aims to select aptamers (consisting of small single-stranded oligonucleotides capable of binding to target molecules with great affinity and specificity, due to the specific secondary and/ or tertiary structures they can acquire) as an alternative in the treatment of colibacillosis. The present experimental study, which is on the APTAcoli agenda, focused on the optimization of a molecular methodology - Multiplex PCR - for the detection of the main virulence factors of ETEC to be used in an epidemiological study to characterize fecal samples from pigs in Portuguese farms. After using different optimization techniques, the results were two multiplex PCR amplification sets, one for amplification of the main toxigenic factors of ETEC (STa, STb, LT and STx2e) and another for amplification of the main adhesion factors (F4, F5, F6, F18 and F41).