Impact of different storage conditions of formamide in the quality of sequences

Moura, Daniela; Pina, Maria João; Pereira, Cátia; Baixia, Márcia; Leal, Rafaela; Sousa, Sónia; Vieira, Filipa Quintela; Silva, Regina; Cirnes, Luís

http://hdl.handle.net/10400.22/20175

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Authors

Vieira, Filipa Quintela

Silva, Regina

Cirnes, Luís

Abstract(s)

Obtaining high quality sequences is essential to proper reading and interpretation. A crucial step for this goal is the denaturation of sequencing products which is usually performed using a denaturing agent such as formamide. It has been described that the reduction of formamide quality may cause irregular cytosine and guanine peaks in an electropherogram.According to manufacturers recomendations, formamide should be stored at -20ºC. Under other conditions such as exposition to light and air and temperature changes for a certain period of time, quality may decrease. Objectives: The purpose of this study was to understand which formamide storage-related variables can be responsible for decreasing sequence quality.

URI

http://hdl.handle.net/10400.22/20175

Citation

Moura, D., Pina, M. J., Pereira, C., Baixia, M., Leal, R., Sousa, S., Vieira, F. Q., Silva, R. A., & Cirnes, L. (2015). Impact of different storage conditions of formamide in the quality of sequences. In R. A. Silva, P. Baylina, F. Q. Vieira, C. Barrias, M. Rubim, T. Coelho, C. Prudêncio, & Â. Jesus (Eds.), II Congresso Internacional da Saúde Gaia Porto: Livro de Resumos (1st ed., Vol. 1, pp. 122). Escola Superior de Tecnologia da Saúde do Instituto Politécnico do Porto.