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Impact of different storage conditions of formamide in the quality of sequences

2015-11Conference object Open access
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dc.contributor.authorMoura, Daniela
dc.contributor.authorPina, Maria João
dc.contributor.authorPereira, Cátia
dc.contributor.authorBaixia, Márcia
dc.contributor.authorLeal, Rafaela
dc.contributor.authorSousa, Sónia
dc.contributor.authorVieira, Filipa Quintela
dc.contributor.authorSilva, Regina
dc.contributor.authorCirnes, Luís
dc.date.accessioned2022-03-09T18:03:08Z
dc.date.available2022-03-09T18:03:08Z
dc.date.issued2015-11
dc.description.abstractObtaining high quality sequences is essential to proper reading and interpretation. A crucial step for this goal is the denaturation of sequencing products which is usually performed using a denaturing agent such as formamide. It has been described that the reduction of formamide quality may cause irregular cytosine and guanine peaks in an electropherogram.According to manufacturers recomendations, formamide should be stored at -20ºC. Under other conditions such as exposition to light and air and temperature changes for a certain period of time, quality may decrease. Objectives: The purpose of this study was to understand which formamide storage-related variables can be responsible for decreasing sequence quality.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMoura, D., Pina, M. J., Pereira, C., Baixia, M., Leal, R., Sousa, S., Vieira, F. Q., Silva, R. A., & Cirnes, L. (2015). Impact of different storage conditions of formamide in the quality of sequences. In R. A. Silva, P. Baylina, F. Q. Vieira, C. Barrias, M. Rubim, T. Coelho, C. Prudêncio, & Â. Jesus (Eds.), II Congresso Internacional da Saúde Gaia Porto: Livro de Resumos (1st ed., Vol. 1, pp. 122). Escola Superior de Tecnologia da Saúde do Instituto Politécnico do Porto.pt_PT
dc.identifier.isbn978-989-20-6340-9
dc.identifier.urihttp://hdl.handle.net/10400.22/20175
dc.language.isoengpt_PT
dc.publisherEscola Superior de Tecnologia da Saúde do Instituto Politécnico do Portopt_PT
dc.relation.ispartofseriesP;28
dc.titleImpact of different storage conditions of formamide in the quality of sequencespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage122pt_PT
oaire.citation.titleII Congresso Internacional da Saúde Gaia Porto: Livro de Resumospt_PT
oaire.citation.volume1pt_PT
person.familyNameQuintela Vieira
person.familyNameSilva
person.familyNameCirnes
person.givenNameAna Filipa
person.givenNameRegina
person.givenNameLuís
person.identifier.ciencia-id871B-D071-507D
person.identifier.ciencia-idA412-0AC5-0780
person.identifier.orcid0000-0003-0130-7664
person.identifier.orcid0000-0002-8373-1217
person.identifier.orcid0000-0002-2348-5878
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication47123d10-a0ed-47cc-a751-721489a13446
relation.isAuthorOfPublication60471846-7876-49e1-b405-6ffe770795ae
relation.isAuthorOfPublication17b01de2-29be-4571-8edd-d7fb6154b26c
relation.isAuthorOfPublication.latestForDiscovery47123d10-a0ed-47cc-a751-721489a13446

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