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Advisor(s)
Abstract(s)
Nowadays, the ageing of population is conducting to a rise in the number of individuals with age-related illnesses, in which neurodegenerative diseases (ND) are included. Regardless of many variations in etiology and diverse mechanisms of cell injury, most of ND share high levels of oxidative stress, which have been highly referred not only as an underlying factor, but also as a feature. Thus, an actual challenge is to evaluate the role of antioxidants on oxidative stress states and evaluate the magnitude of possible therapeutic effects of these agents. In the present study, the toxic effect of hydrogen peroxide (H2O2) and the antioxidative function of vitamin C were investigated using Saccharomyces cerevisiae as a model. Results were achieved by exposing the yeast model to different concentrations of H2O2 and vitamin C, itself or combined, and colony forming units counting after 48 h incubation at 30ºC in Yeast Extract Peptone Dextrose agar plates. The results demonstrated that H2O2 exposure decreased yeast cells viability in a dose-dependent manner and that, at an optimal concentration, vitamin C was able to revert its effects. These results improved the understanding of the reversal effect of antioxidant treatment and, therefore, may be helpful on providing insights on a natural antioxidant-based therapy for ND.
Description
Keywords
Neurodegenerative diseases Oxidative stress Saccharomyces cerevisiae Vitamin C
Citation
Costa, A. R., Cunha, S., Nascimento, A. P., Vieira, M., & Prudêncio, C. (2018). The effect of high concentrations of vitamin C on a H2O2—Oxidative stress induced yeast model. Book of abstracts of the III Encontro de Biotecnologia Medicinal/ I Iberian Congress on Medicinal Biotechnology, 51. https://paginas.ess.ipp.pt/ebtm/2018/3EBtM_BookOfAbstracts.pdf
Publisher
Escola Superior de Saúde P.Porto