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Enhancing the detection of Dinophysis spp. using electrochemical genosensors

2023-07-09Conference object Open access
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dc.contributor.authorPereira, Eduarda
dc.contributor.authorSilva, Aurora
dc.contributor.authorMorais, Stephanie L.
dc.contributor.authorCosta-Rama, Estefanía
dc.contributor.authorMoreira, Patrícia R.
dc.contributor.authorFraga-Corral, M.
dc.contributor.authorTorrado, Ana M.
dc.contributor.authorRodríguez, Francisco
dc.contributor.authorBarros, Piedade
dc.contributor.authorCruz, Agostinho
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorPrieto, M. A.
dc.contributor.authorSimal-Gandara, J.
dc.contributor.authorSilva, Nádia F. D.
dc.contributor.authorSantos, Marlene
dc.contributor.authorBarroso, M. Fátima
dc.date.accessioned2023-10-06T15:59:52Z
dc.date.available2023-10-06T15:59:52Z
dc.date.issued2023-07-09
dc.description.abstractHarmful algal blooms (HABs) pose a significant threat to the environment and public health. These blooms are defined by an accumulation of microscopic algae in water, and they can occur inlakes, rivers, estuaries, orcoastal areas. Factors like the unregulated runoff of agricultural and industrial wastes into the aquatic environment are believed to have transformed these ecosystems into favorable habitats for algae growth and proliferation. As a result, the frequency of these blooms is rising worldwide. Although these blooms are mostly harmless, certain species, namely dinoflagellates from the genus Dinophysis, produce toxins that pose a risk for human health. Therefore, the need for technological developments towards fast and precise detection of these toxin-producing microalgae is critical to prevent socio economical damages, as well as to assess the ecological status of marine ecosystems. In this work, an analytical approach based on an electrochemical genosensor device was developed to create a low-cost platform able to detect two dinoflagellate species from the genus Dinophysis: D.acuminataand D.acuta. The design of the DNA-based sensor involved three key steps: i) Sensing phase: consisted by a mixed self-assembled monolayer composed by a linear DNA capture probe and mercaptohexanol on to the disposable screen-printed gold electrodessurface; ii) Hybridization of complementary DNA sequence by using a sandwich format assay with enzymatic labels and iii) Electrochemical detection by chronoamperometry using an enzymatic scheme to amplify the electrochemical signal. The best analytical conditions used to study the relationship between electrochemical signal and DNA target concentration, to produce the best electrochemical genosensor device. Molecular biology tools, namely Polymerase Chain Reaction (PCR), will be used for further validation of the electrochemical genosensor to confirm its reliability. These advancements in analytical technologies contribute to the on going efforts in environmental management and public health protection by providing effective means for detectingand mitigating the risks associated with HABs. Further research and widespread implementation of these methods are required to ensure the safety and sustainability of aquatic ecosystems, safeguard public health, and facilitate proactive environmental management practices.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPereira, E., Silva, A., Morais, S. L., Costa-Rama, E., Moreira, P. R., Fraga-Corral, M., Torrado, A. M., Rodríguez, F., Barros, P., Cruz, A., Delerue-Matos, C., Prieto, M. A., Simal-Gandara, J., Silva, N. F. D., Santos, M., & Barroso, M. F. (2023). Enhancing the detection of Dinophysis spp. Using electrochemical genosensors. Proceedings of Research and Practice in Allied and Environmental Health, 1(1), 27. https://doi.org/10.26537/prpaeh.v1i1.5172pt_PT
dc.identifier.doi10.26537/prpaeh.v1i1.5172pt_PT
dc.identifier.issn2975-9420
dc.identifier.urihttp://hdl.handle.net/10400.22/23650
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://parc.ipp.pt/index.php/PRPAEH/article/view/5172pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDinoflagellatespt_PT
dc.subjectDinophysis spp.pt_PT
dc.subjectElectrochemistrypt_PT
dc.subjectGenosensorpt_PT
dc.subjectMolecular biologypt_PT
dc.titleEnhancing the detection of Dinophysis spp. using electrochemical genosensorspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
oaire.citation.startPage27pt_PT
oaire.citation.titleProceedings of Research and Practice in Allied and Environmental Health 2023pt_PT
oaire.citation.volume1 (1)pt_PT
person.familyNameGonçalves de Barros
person.familyNameCruz
person.familyNameSantos
person.givenNamePiedade Aurora
person.givenNameAgostinho
person.givenNameMarlene
person.identifier1508370
person.identifier.ciencia-idD210-6003-454B
person.identifier.ciencia-id8311-B967-31C4
person.identifier.orcid0000-0002-2635-251X
person.identifier.orcid0000-0002-1157-8196
person.identifier.orcid0000-0001-5020-5942
person.identifier.scopus-author-id23981959400
person.identifier.scopus-author-id57110502000
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationa85b368a-8f13-45eb-a744-7c5810a38e2c
relation.isAuthorOfPublicationd7ec3c5d-b3e0-4e34-8e24-4d1588876120
relation.isAuthorOfPublication8ce9ee39-a4c6-46ae-99e2-49397b550f1b
relation.isAuthorOfPublication.latestForDiscovery8ce9ee39-a4c6-46ae-99e2-49397b550f1b

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