Publicação
Potential therapeutic application of chestnut shells in oral mucositis treatment: Green extraction of polyphenols
| dc.contributor.author | Ferreira, Ana Sofia | |
| dc.contributor.author | Silva, Ana Margarida | |
| dc.contributor.author | Pinto, Diana | |
| dc.contributor.author | Ferraz, Ricardo | |
| dc.contributor.author | Moreira, Manuela M. | |
| dc.contributor.author | Švarc-Gajić, Jaroslava | |
| dc.contributor.author | Costa, Paulo | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.contributor.author | Rodrigues, Francisca | |
| dc.date.accessioned | 2024-05-29T13:51:59Z | |
| dc.date.available | 2024-05-29T13:51:59Z | |
| dc.date.issued | 2023-03-21 | |
| dc.description.abstract | Oral mucositis (OM), a common side effect of oncological treatment, is an inflammatory condition characterized by painful ulcerations, difficulty swallowing and speaking, and increased risk of infection, which can compromise the primary treatment outcome. The use of natural antioxidants to inhibit theredox imbalance responsible for OM development has emerged as an interesting approach for OM prevention and treatment. This study aims to explore chestnut (Castana sativa) shells (by-products) aspotential active ingredient against OM [2]. To recover antioxidants using sustainable technology, chestnut shells were extracted using Subcritical Water Extraction (SWE) at different temperatures (110-180 °C). The extracts were evaluated against microorganisms present in the oral cavity as well as in human oral cell lines (TR146 and HSC3). The optimal extraction temperature was 110 °C, exhibiting thehighest phenolic content and the best antioxidant/antiradical activities and scavenging efficiencies against HOCl (IC50 = 4.47 μg/mL) and ROO● (0.73 μmol TE/mg DW). High concentrations of phenolicacids (e.g., gallic and protocatechuic acid) and flavanoids (catechin, epicatechin and rutin) characterizedthe phenolic profile. Antimicrobial activity against several microorganisms present in the oral cavity during OM, such as Streptococcus, Staphylococcus, Enterococcus, and Escherichia, was also demonstrated. The effects on HSC3 and TR146 cell lines revealed that the extract prepared at 110 °C showed the lowest IC50 (1325.03 and 468.15 µg/mL, respectively). Therefore, this extract was selectedfor encapsulation and incorporation into an oral film as buccal drug delivery system. Methocel 1000 demonstrated ideal physicochemical properties from different set of polymers, such as thickness, mass, content uniformity, dissolution time in artificial saliva, superficial pH, folding, tensile strength, and elongation. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Ferreira, A. S., Silva, A. M., Pinto, D., Ferraz, R., Moreira, M. M., Švarc-Gajić, J., Costa, P., Delerue-Matos, C., & Rodrigues, F. (2023). Potential therapeutic application of chestnut shells in oral mucositis treatment: Green extraction of polyphenols. Dare2Change 2023 Innovation Driven Agrifood Business Abstract Book, 133–134. | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/25594 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Associação Colab4Food - Laboratório Colaborativo Para Inovação da Indústria AgroAlimentar and INIAV – Instituto Nacional de Investigação Agrária e Veterinária, I.P. | pt_PT |
| dc.relation | The authors thank Sortegel (Sortes, Portugal) for the samples. This work was financially supported by national funds (UIDB/50006/2020), and projects PTDC/ASP-AGR/29277/2017 and 5537 DRI, Sérvia 2020/21 from Portuguese-Serbia Bilateral Cooperation, supported by national funds by FCT/MCTES and co- supported by FEDER throughout COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI-01-0145-FEDER-029277). Ana Sofia Ferreira (SFRH/BD/7519/2020), Diana Pinto (SFRH/BD/144534/2019) and Ana Margarida Silva (SFRH/BD/144994/2019) are thankful for their Ph.D. grants financed by POPH-QREN and subsidized by the European Science Foundation and Ministério da Ciência, Tecnologia e Ensino Superior. Francisca Rodrigues (CEECIND/01886/2020) and Manuela M. Moreira (CEECIND/02702/2017) are thankful for their contracts financed by FCT/MCTES—CEEC Individual Program Contract. Jaroslava Švarc-Gajić is grateful to the Science Fund of the Republic of Serbia (Grant No. 7747845, In situ pollutants removal from waters by sustainable green nano-technologies— CleanNanoCatalyze), and to the Ministry of education, science and technological development of the Republic of Serbia (Grant No. 451-03-68/2020–14/200134). | pt_PT |
| dc.relation.publisherversion | https://dare2change.pt/images/abstracts_2023/AbstractBook%20DARE2CHANGE%202023.pdf | pt_PT |
| dc.title | Potential therapeutic application of chestnut shells in oral mucositis treatment: Green extraction of polyphenols | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Porto | pt_PT |
| oaire.citation.endPage | 134 | pt_PT |
| oaire.citation.startPage | 133 | pt_PT |
| oaire.citation.title | Dare2Change 2023 Innovation Driven Agrifood Business Abstract Book | pt_PT |
| person.familyName | Ferraz | |
| person.givenName | Ricardo | |
| person.identifier.ciencia-id | 001E-71CE-F92D | |
| person.identifier.orcid | 0000-0002-1761-117X | |
| person.identifier.rid | G-5639-2011 | |
| person.identifier.scopus-author-id | 24464208500 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | a5a8faa7-12a5-4b1c-bced-44c895677397 | |
| relation.isAuthorOfPublication.latestForDiscovery | a5a8faa7-12a5-4b1c-bced-44c895677397 |