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- Contaminação do ar e superfíciesPublication . Afonso, Joana; Vieira Da Silva, ManuelaOs estabelecimentos de panificação e pastelaria são estabelecimentos do sector alimentar com grande influência na alimentação humana visto os seus géneros alimentícios serem a base da alimentação de grande parte da população. Aliando a abrangência em termos de alimentos confeccionados ao maior número de pessoas que recorrem aos seus produtos, é essencial garantir a qualidade destes alimentos.
- Avaliação da exposição ocupacional a vibrações transmitidas ao sistema corpo inteiro: estudo preliminar em motoristas de pesados de mercadoriasPublication . Costa, Sofia; Santos, Joana; Silva, Maria V.; Neves, PaulaAs vibrações transmitidas ao corpo inteiro (VCI) são um agente físico que afecta os condutores de camiões e apresenta consequências para a sua saúde, estando fortemente associada à dor lombar. No presente estudo seleccionou-se um motorista que conduz um veículo pesado de mercadorias com classificação N3 e que efectua rotas de pequeno curso no Norte de Portugal. As medições realizaram-se em dois dias representativos da actividade semanal, em duas rotas distintas (Rota 1 e 2), de acordo com a metodologia definida no Decreto-Lei n.º 46/2006 de 24 de Fevereiro e na NP ISO 2631-1 de 2007. Foi utilizado um analisador de vibrações, sendo o acelerómetro triaxial fixado ao assento do veículo, de forma a quantificar a aceleração eficaz nos três eixos ortogonais x, y e z. Complementarmente ao processo de amostragem, aplicou-se um questionário a sete motoristas. Os resultados demonstram a dominância da direcção vertical (z) com picos de aceleração na frequência de 1,6Hz. A degradação do piso e a ausência de carga do veículo foram identificados como factores responsáveis pelo aumento dos níveis vibracionais. Relativamente à exposição do trabalhador, obteve-se valores de A(8) Rota 1 de 0,22 m/s2 e A(8) Rota 2 de 0,14 m/s2, sendo o A(8) semanal de 0,19 m/s2, verificando-se que os valores obtidos são inferiores ao valor de acção de exposição preconizado na legislação nacional (0,5m/s2). Quanto aos sintomas manifestados pelos motoristas destacam-se: dor lombar (43%), fadiga (71%), irritabilidade (57%) e dores de cabeça (57%). Apesar dos valores obtidos evidenciarem que o trabalhador não se encontra exposto, segundo o preconizados na legislação, devem ser implementadas medidas que visem a manutenção e conservação dos pisos das vias, manutenção dos veículos e elaboração de planos de formação que abordem o tipo de condução e as posturas a adoptar para salvaguarda do bem-estar e conforto dos trabalhadores.
- Lichens as bio indicators of atmospheric pollution in Porto, PortugalPublication . Freitas, Marisa; Costa, Natália; Rodrigues, Matilde; Marques, Joana; Vieira Da Silva, ManuelaThe emission of air pollutants mainly from anthropogenic sources has led to the degradation of air quality. These pollutants determine the occurrence or worsening of respiratory disorders. Biomonitoring provides information on the quality of the environment or its modifications, having been used as an alternative to monitoring of chemical pollutants. The variation of the biodiversity of lichens can be used as a warning to check if other biological systems are being affected by atmospheric pollution. Lichens have been used as bioindicators, since they have differential sensitivity to air pollution. The purpose of this study was to assess the diversity and abundance of lichens in different zones of the Porto (North Portugal) with respect to the rates of atmospheric pollutants. Three zones were selected for sampling, represented by urban, suburban and rural zones. LDV was calculated for each zone. The average concentrations of CO, CO2, SO2, NO2, O3 and PM10 were also measured. The most sensitive lichens were present in the area with the highest LDV. In places where there were higher concentrations of pollutants, namely NO2 and SO2 a lower LDV. This study suggests that lichens can be used as prevention systems to protect public health, in particular diseases related to air pollution and as a potential alternative or complement to expensive chemical monitoring equipment.
- Application of real-time PCR in the assessment of the toxic cyanobacterium cylindrospermopsis raciborskii abundance and toxicological potentialPublication . Moreira, Cristiana; Martins, António; Azevedo, Joana; Freitas, Marisa; Regueiras, Ana; Vale, Micaela; Antunes, Agostinho; Vasconcelos, VítorCyanobacteria are prokaryotic photosynthetic microorganisms that pose a serious threat to aquatic environments because they are able to form blooms under eutrophic conditions and produce toxins. Cylindrospermopsis raciborskii is a planktonic heterocystous filamentous cyanobacterium initially assigned to the tropics but currently being found in more temperate regions such as Portugal, the southernmost record for this species in Europe. Cylindrospermopsin originally isolated from C. raciborskii is a cytotoxic alkaloid that affects the liver, kidney, and other organs. It has a great environmental impact associated with cattle mortality and human morbidity. Aiming in monitoring this cyanobacterium and its related toxin, a shallow pond located in the littoral center of Portugal, Vela Lake, used for agriculture and recreational purposes was monitored for a 2-year period. To accomplish this, we used the real-time PCR methodology in field samples to quantify the variation of specific genetic markers with primers previously described characterizing total cyanobacteria (16S rRNA), C. raciborskii (rpoC1), and cylindrospermopsin synthetase gene (pks). The results report the high abundance of both cyanobacteria and C. raciborskii in Vela Lake, with C. raciborskii representing 0.4% to 58% of the total cyanobacteria population. Cylindrospermopsin synthetase gene was detected in one of the samples. We believe that with the approach developed in this study, it will be possible to monitor C. raciborskii population dynamics and seasonal variation, as well as the potential toxin production in other aquatic environments.
- Influence of mixtures of acenaphthylene and benzo[a] anthracene on their degradation by Pleurotus ostreatus in sandy soilPublication . Rocha, Inês; Pinto, Edgar; Ferreira, Isabel M. P. L. V. O.; Vieira Da Silva, Manuela; Oliveira, Rui S.Purpose Polycyclic aromatic hydrocarbons (PAHs) are a class of organic compounds commonly found as soil contaminants. Fungal degradation is considered as an environmentally friendly and cost-effective approach to remove PAHs from soil. Acenaphthylene (Ace) and Benzo[a]anthracene (BaA) are two PAHs that can coexist in soils; however, the influence of the presence of each other on their biodegradation has not been studied. The biodegradation of Ace and BaA, alone and in mixtures, by the white rot fungus Pleurotus ostreatus was studied in a sandy soil. Materials and methods Experimental microcosms containing soil spiked with different concentrations of Ace and BaAwere inoculated with P. ostreatus. Initial (t 0) and final (after 15 days of incubation) soil concentrations of Ace and BaA were determined after extraction of the PAHs. Results and discussion P. ostreatus was able to degrade 57.7% of the Ace in soil spiked at 30 mg kg−1 dry soil and 65.8% of Ace in soil spiked at 60 mg kg−1 dry soil. The degradation efficiency of BaA by P. ostreatus was 86.7 and 77.4% in soil spiked with Ace at 30 and 60 mg kg−1 dry soil, respectively. After 15 days of incubation, there were no significant differences in Ace concentration between soil spiked with Ace and soil spiked with Ace + BaA, irrespective of the initial soil concentration of both PAHs. There were also no differences in BaA concentration between soil spiked with BaA and soil spiked with BaA + Ace. Conclusions The results indicate that the fungal degradation of Ace and BaA was not influenced by the presence of each other’s PAH in sandy soil. Bioremediation of soils contaminated with Ace and BaA using P. ostreatus is a promising approach to eliminate these PAHs from the environment.
- Risk criteria in occupational environments: critical overview and discussionPublication . Rodrigues, Matilde; Arezes, Pedro; Leão, Celina PintoThe current work can be seen as a starting point for the discussion of the problematic on risk acceptance criteria in occupational environments. Some obstacles to the quantitative acceptance criteria formulation and use were analyzed. A look to the long tradition of major hazards accidents was also performed. This work shows that organizations can have several difficulties in acceptance criteria formulation and that the use of pre-defined acceptance criteria in risk assessment methodologies can be inadequate in some cases. It is urgent to define guidelines that can help organizations in the formulation of risk acceptance criteria for occupational environments.
- Exposure of Lycopersicon Esculentum to Microcystin-LR: Effects in the Leaf Proteome and Toxin Translocation from Water to Leaves and FruitsPublication . Gutiérrez-Praena, Daniel; Campos, Alexandre; Azevedo, Joana; Neves, Joana; Freitas, Marisa; Guzmán-Guillén, Remédios; Cameán, Ana María; Renaut, Jenny; Vasconcelos, VítorNatural toxins such as those produced by freshwater cyanobacteria have been regarded as an emergent environmental threat. However, the impact of these water contaminants in agriculture is not yet fully understood. The aim of this work was to investigate microcystin-LR (MC-LR) toxicity in Lycopersicon esculentum and the toxin accumulation in this horticultural crop. Adult plants (2 month-old) grown in a greenhouse environment were exposed for 2 weeks to either pure MC-LR (100 μg/L) or Microcystis aeruginosa crude extracts containing 100 μg/L MC-LR. Chlorophyll fluorescence was measured, leaf proteome investigated with two-dimensional gel electrophoresis and Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF)/TOF, and toxin bioaccumulation assessed by liquid chromatography-mass spectrometry (LC-MS)/MS. Variations in several protein markers (ATP synthase subunits, Cytochrome b6-f complex iron-sulfur, oxygen-evolving enhancer proteins) highlight the decrease of the capacity of plants to synthesize ATP and to perform photosynthesis, whereas variations in other proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit and ribose-5-phosphate isomerase) suggest an increase of carbon fixation and decrease of carbohydrate metabolism reactions in plants exposed to pure MC-LR and cyanobacterial extracts, respectively. MC-LR was found in roots (1635.21 μg/kg fw), green tomatoes (5.15–5.41 μg/kg fw), mature tomatoes (10.52–10.83 μg/kg fw), and leaves (12,298.18 μg/kg fw). The results raise concerns relative to food safety and point to the necessity of monitoring the bioaccumulation of water toxins in agricultural systems affected by cyanotoxin contamination.
- Evaluation of the noise exposure of symphonic orchestra musiciansPublication . Rodrigues, Matilde; Freitas, Marisa; Neves, Paula; Vieira Da Silva, ManuelaFor musicians, the impact of noise exposure is not yet fully characterized. Some inconsistencies can be found in the methodology used to evaluate noise exposure. This study aims to analyze the noise exposure of musicians in a symphonic orchestra to understand their risk for hearing loss, applying the methodology proposed by ISO 9612:2009. Noise levels were monitored among musicians during the rehearsal of eight different repertoires. Test subjects were selected according to their instrument and position in the orchestra. Participants wore noise dosimeters throughout the rehearsals. A sound meter was used to analyze the exposure of the conductor. The results showed that musicians are exposed to high noise levels that can damage hearing. Brass, woodwind and percussion and timpani musicians were exposed to noise levels in excess of the upper exposure action level of 85 dB (A), while the other instrumental groups had a lower exposure action level of 80 dB (A). Percussion musicians were exposed to high peak noise levels of 135 dB (C). Sound levels varied by instrument, repertoire and position. Octave frequency analyses showed differences among musicians. This study suggests that musicians are at risk for hearing loss. There is a need for more effective guidelines applicable to all countries, which should define standardized procedures for determining musician noise exposure and should allow exposure level normalization to the year, including different repertoires.
- Effects of storage, processing and proteolytic digestion on microcystin-LR concentration in edible clamsPublication . Freitas, Marisa; Azevedo, Joana; Carvalho, António Paulo; Campos, Alexandre; Vasconcelos, VítorAccumulation of microcystin-LR (MC-LR) in edible aquatic organisms, particularly in bivalves, is widely documented. In this study, the effects of food storage and processing conditions on the free MC-LR concentration in clams (Corbicula fluminea) fed MC-LR-producing Microcystisaeruginosa (1 × 105 cell/mL) for four days, and the bioaccessibility of MC-LR after in vitro proteolytic digestion were investigated. The concentration of free MC-LR in clams decreased sequentially over the time with unrefrigerated and refrigerated storage and increased with freezing storage. Overall, cooking for short periods of time resulted in a significantly higher concentration (P < 0.05) of free MC-LR in clams, specifically microwave (MW) radiation treatment for 0.5 (57.5%) and 1 min (59%) and boiling treatment for 5 (163.4%) and 15 min (213.4%). The bioaccessibility of MC-LR after proteolytic digestion was reduced to 83%, potentially because of MC-LR degradation by pancreatic enzymes. Our results suggest that risk assessment based on direct comparison between MC-LR concentrations determined in raw food products and the tolerable daily intake (TDI) value set for the MC-LR might not be representative of true human exposure.
- Multilevel model of safety climate for furniture industriesPublication . Rodrigues, Matilde; Arezes, Pedro; Leão, Celina P.BACKGROUND: Furniture companies can analyze their safety status using quantitative measures. However, the data needed are not always available and the number of accidents is under-reported. Safety climate scales may be an alternative. However, there are no validated Portuguese scales that account for the specific attributes of the furniture sector. OBJECTIVE: The current study aims to develop and validate an instrument that uses a multilevel structure to measure the safety climate of the Portuguese furniture industry. METHODS: The Safety Climate in Wood Industries (SCWI) model was developed and applied to the safety climate analysis using three different scales: organizational, group and individual. A multilevel exploratory factor analysis was performed to analyze the factorial structure. The studied companies’ safety conditions were also analyzed. RESULTS: Different factorial structures were found between and within levels. In general, the results show the presence of a group-level safety climate. The scores of safety climates are directly and positively related to companies’ safety conditions; the organizational scale is the one that best reflects the actual safety conditions. CONCLUSIONS: The SCWI instrument allows for the identification of different safety climates in groups that comprise the same furniture company and it seems to reflect those groups’ safety conditions. The study also demonstrates the need for a multilevel analysis of the studied instrument.