Browsing by Author "Santos, C."
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- Estudo do comportamento do ouvido médio considerando a presença de fluidoPublication . Garbe, C.; Gentil, Fernanda; Parente, M.; Martins, P.; Santos, C.; Branco, C.; Paço, J.; Natal Jorge, R.O objetivo deste trabalho foi estudar a influência de diferentes fluidos dentro e fora da cavidade timpânica no comportamento biomecânico do ouvido médio. O modelo computacional 3 D da cadeia tímpano‐ossicular foi construído através de imagens de tomografia axial computorizada (TAC). Após a construção do modelo da cadeia tímpano‐ossicular, para a parte interna da membrana timpânica foi criada a caixa timpânica, que inclui toda a cadeia ossicular, e para a parte externa da membrana foi criada uma passagem, simulando o canal auditivo externo. A discretização do modelo foi feita utilizando o método dos elementos finitos, com base no software ABAQUS. As propriedades mecânicas foram extraídas de trabalhos anteriores. Foram obtidos os deslocamentos do umbo e de um ponto central da platina do estribo, para um nível de pressão sonora de 105 dB aplicada na membrana timpânica, comparando a cadeia tímpano‐ossicular e os diferentes níveis de pressão externa e interna do ouvido médio (fluido líquido e fluido gasoso). Os resultados demonstram que o comportamento biomecânico do ouvido médio é diferente para os diferentes níveis de pressões testadas neste modelo. Quando utilizada uma pressão de fluido líquido, os deslocamentos são de amplitude inferior quando comparados com os resultados obtidos com pressão de ar.
- Novel Prostate Specific Antigen plastic antibody designed withcharged binding sites for an improved protein binding and itsapplication in a biosensor of potentiometric transductionPublication . Rebelo, Tânia S. C. R.; Santos, C.; Costa-Rodrigues, João; Fernandes, M.H.; Noronha, João P. C.; Sales, M. Goreti F.This work shows that the synthesis of protein plastic antibodies tailored with selected charged monomersaround the binding site enhances protein binding. These charged receptor sites are placed over a neutralpolymeric matrix, thus inducing a suitable orientation the protein reception to its site. This is confirmed bypreparing control materials with neutral monomers and also with non-imprinted template. This concepthas been applied here to Prostate Specific Antigen (PSA), the protein of choice for screening prostate can-cer throughout the population, with serum levels >10 ng/mL pointing out a high probability of associatedcancer.Protein Imprinted Materials with charged binding sites (C/PIM) have been produced by surfaceimprinting over graphene layers to which the protein was first covalently attached. Vinylben-zyl(trimethylammonium chloride) and vinyl benzoate were introduced as charged monomers labellingthe binding site and were allowed to self-organize around the protein. The subsequent polymerizationwas made by radical polymerization of vinylbenzene. Neutral PIM (N/PIM) prepared without orientedcharges and non imprinted materials (NIM) obtained without template were used as controls.These materials were used to develop simple and inexpensive potentiometric sensor for PSA. Theywere included as ionophores in plasticized PVC membranes, and tested over electrodes of solid or liq-uid conductive contacts, made of conductive carbon over a syringe or of inner reference solution overmicropipette tips. The electrodes with charged monomers showed a more stable and sensitive response,with an average slope of -44.2 mV/decade and a detection limit of 5.8 × 10−11mol/L (2 ng/mL). The cor-responding non-imprinted sensors showed lower sensitivity, with average slopes of -24.8 mV/decade.The best sensors were successfully applied to the analysis of serum, with recoveries ranging from 96.9to 106.1% and relative errors of 6.8%.
- Spirulina in the creation of new nutritionally fortified productsPublication . Santos, C.; Caetano, N.(Introduction) Protein sources will experience great increase in demand due primarly to the growing population and the change in consumer's habbits. Animal proteins account for about 40% of global protein consumption, however, they are associated with a high environmental footprint. Microalgae are emerging as promising sustainable alternatives. Beyond their rich protein content, Spirulina has a spectrum of dietary bioactive compounds, essential fatty acids, and vitamins, positioning it as a valuable addition to mainstream foods, with the potential to increase their nutritional value and mitigate environmental impact.
- The human otitis media with effusion: a numerical-based studyPublication . Areias, B.; Parente, M. P. L.; Santos, C.; Gentil, Fernanda; Natal Jorge, R. M.Otitis media is a group of inflammatory diseases of the middle ear. Acute otitis media and otitis media with effusion (OME) are its two main types of manifestation. Otitis media is common in children and can result in structural alterations in the middle ear which will lead to hearing losses. This work studies the effects of an OME on the sound transmission from the external auditory meatus to the inner ear. The finite element method was applied on the present biomechanical study. The numerical model used in this work was built based on the geometrical information obtained from The visible ear project. The present work explains the mechanisms by which the presence of fluid in the middle ear affects hearing by calculating the magnitude, phase and reduction of the normalized umbo velocity and also the magnitude and phase of the normalized stapes velocity. A sound pressure level of 90 dB SPL was applied at the tympanic membrane. The harmonic analysis was performed with the auditory frequency varying from 100 Hz to 10 kHz. A decrease in the response of the normalized umbo and stapes velocity as the tympanic cavity was filled with fluid was obtained. The decrease was more accentuated at the umbo.