Browsing by Author "Costa, J."
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- Adipocyte proteome and secretome influence inflammatory and hormone pathways in gliomaPublication . Almeida, Joana; Costa, J.; Coelho, Pedro; Cea, V.; Galesio, M.; Noronha, J. P.; Diniz, M. S.; Prudêncio, Cristina; Soares, R.; Sala, C.; Fernandes, RúbenGliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study, we explore the relationship between obesity and glioma in a cellular model. Thus, we have study the influence of adipocytes secretome on glioma cell line GL261. Using the 3T3-L1 adipocyte cell line, and its conditioned medium (adipokines-enriched medium), we showed that adipocyte-released factors relate with glioma angiogenic, growth, hormones and metabolic behavior by MALDI-TOF-MS and proteomic array analysis. In a first view, STI1, hnRNPs and PGK1 are under expressed on CGl. Similarly, both carbonic anhydrase and aldose reductase are even suppressed in glioma cells that grown under adipokines-enriched environment. Contrariwise, RFC1, KIF5C, ANXA2, N-RAP and RACK1 are overexpressed in GL261 cell the in the presence of the adipokines-enriched medium. We further identified the factors that are released by adipocyte cells, and revealed that several pro-inflammatory and angiogenic factors, such as IL-6, IL-11, LIF, PAI-1, TNF-α, endocan, HGF, VEGF IGF-I, were secreted to the medium into a high extent, whereas TIMP-1 and SerpinE1 were under expressed on CGl. This study discloses an interesting in vitro model for the study of glioma biology under a "obesity" environment, that can be explored for the understanding of cancer cells biology, for the search of biomarkers, prognostic markers and therapeutic approaches.
- Differential imune response to vitamin A in B16-F10 malignant melanocytesPublication . Oliveira, S.; Coelho, Pedro; Costa, J.; Prudêncio, Cristina; Soares, R.; Guerreiro, S. G.; Fernandes, RúbenMelanoma is an aggressive form of skin cancer with a poor prognosis, due to its refractory behavior to radiation and chemotherapy. Although the diagnosis is straightforward, there are many disagreements regarding its treatment and surveillance. In order to surpass some of the limitations addressed to the treatment, preventive methods like antioxidant vitamins are nowadays a relevant field of research, as well as immunostimulation by external agents. Despite the knowledge about melanoma biology, pathogenesis and developed therapies, is important to understand the effect of vitamin A in order to suggest alternatives to conventional therapies, which are known to be ineffective against melanoma.
- Effect of the pharmaceuticals metformin, escitalopram and furosemide in cyanobacteria growthPublication . Oliveira, L.; Pereira, R.; Costa, J.; Barros, PiedadeWorldwide one of the most prevalent water problems is eutrophication, a result of high nutrient concentration, mainly phosphorus and nitrogen. In freshwater environments, anthropogenic inputs of nutrients and other substances are a major contributing to eutrophication and consequent formation of cyanobacteria and algal blooms. The formation of cyanobacteria blooms can result in toxin production and have ecological and human health impacts. Another emerging water quality concern is the impact of personal care products and pharmaceuticals in the environment. In eutrophic waters the increased availability of pharmaceuticals can interfere with cyanobacteria cells and disrupt or enhance cyanobacteria growth. This study describes the effect of metformine, escitalopram and furosemide in the cyanobacteria Microcystis aeruginosa. Cyanobacteria tests were conducted following the European Guideline (OECD 201) for algal and cyanobacteria growth inhibition test. Cyanobacteria were incubated in microplate with pharmaceuticals solutions prepared in Z8 medium under continuous light and shaking at 22ºC. Results were quantified in terms of average growth rates calculated from cell numbers based on OD determination at 24h period until 120h. Pharmaceuticals used were metformin an anti-diabetic, escitalopram an antidepressive and furosemide a diuretic in ten concentrations. At 120h exposure and at the higher concentration tested the three pharmaceuticals (metformin-10mg/mL; 100% inhibition; escitalopram- 0.1mg/ml; 100% inhibition and furosemide-0.4 mg/mL; 50% inhibition) inhibited Microcystis growth rate. Escitalopram response showed hormesis but at higher concentrations it was more toxic than metformin and furosemide. Time related response was different between pharmaceuticals. The three pharmaceuticals interfere with Microcystis growth rate in different ways.
- Is the MTHFR C677T polymorphism associated with obesity risk? – a meta-analysis.Publication . Costa, J.; Oliveira, R.; Prudêncio, Cristina; Fernandes, RúbenOverweight and obesity are a major worldwide health problem and its incidence is increasing every year. Methylenetetrahydrofolate reductase (MTHFR) plays an important role in folate metabolism and as a regulator of DNA methylation, synthesis, and repair. MTHFR gene is polymorphic at nucleotides 677 (C→T) and 1298 (A→C). MTHFR C677T polymorphism results in alloenzymes with decreased activity and several studies have pointed to association between the MTHFR C677T polymorphism and overweight/obesity risk.
- Metabolic syndrome and inflammation: is there a microvascular and an incretin system impairment in the gastrointestinal tract?Publication . Costa, J.; Almeida, J.; Coelho, Pedro; Oliveira, S.; Prudêncio, Cristina; Soares, R.; Gomes-Guerreiro, S.; Fernandes, RúbenMetabolic syndrome is a multifactorial disorder characterized by increased plasma levels of glucose, cholesterol and triglycerides, but also overweight and obesity promoted by increase of body fat mass, alterations in oxidative stress, chronic low grade inflammation and resistance to insulin leading to risk of cardiovascular diseases. The stomach and the intestine have an essential role in metabolism with functions of digesting food and absorption of nutrients. Also, the intestine produces incretin hormones, such as GLP-1 which regulates glucose metabolism and processes of the gastrointestinal tract.
- Obesity and brain cancer: proteomic analyzes of the influence of the adipocyte secretome on glioma Gl261 cellsPublication . Fernandes, Rúben; Costa, J.; Almeida, Joana; Coelho, Pedro; Cea, V.; Galésio, M.; Diniz, MS.; Prudêncio, Cristina; Sala, C.Glioma is the most frequent form of malignant brain tumor in the adults and childhood. There is a global tendency toward a higher incidence of gliomas in highly developed and industrialized countries. Simultaneously obesity is reaching epidemic proportions in such developed countries. It has been highly accepted that obesity may play an important role in the biology of several types of cancer. We have developed an in vitro method for the understanding of the influence of obesity on glioma mouse cells (Gl261).
- The role of MTHFR polymorphisms in breast cancer risk– A metaanalysisPublication . Costa, J.; Oliveira, S.; Oliveira, R.; Prudêncio, Cristina; Fernandes, RúbenBreast cancer incidence has been increasing by more than one million new cases every year and is a primary cause of cancer death among women worldwide. Methylenetetrahydrofolate reductase (MTHFR) plays an important role in folate metabolism and as a regulator of DNA methylation, synthesis, and repair. MTHFR gene is polymorphic at nucleotides 677 (C®T) and 1298 (A®C), resulting in allozymes with decreased activity. Several studies have pointed to association between the MTHFR C677T polymorphism and breast cancer risk.