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Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates

2021-01-15Journal article Restricted access
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dc.contributor.authorMorais, Stephanie L.
dc.contributor.authorBarros, Piedade
dc.contributor.authorSantos, Marlene
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorGomes, Andreia C.
dc.contributor.authorBarroso, M. Fátima
dc.date.accessioned2021-11-24T16:39:44Z
dc.date.available2021-11-24T16:39:44Z
dc.date.issued2021-01-15
dc.description.abstractThis work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a oncentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD <5.2% was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMorais, S. L., Barros, P., Santos, M., Delerue-Matos, C., Gomes, A. C., & Fátima Barroso, M. (2021). Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta, 222, 121416. https://doi.org/10.1016/j.talanta.2020.121416pt_PT
dc.identifier.doi10.1016/j.talanta.2020.121416pt_PT
dc.identifier.issn0039-9140
dc.identifier.urihttp://hdl.handle.net/10400.22/18944
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0039914020307074pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAlexandrium minutumpt_PT
dc.subjectDinoflagellatept_PT
dc.subjectChronoamperometrypt_PT
dc.subjectElectrochemical genosensorpt_PT
dc.subjectSandwich format hybridizationpt_PT
dc.titleElectrochemical genosensor for the detection of Alexandrium minutum dinoflagellatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleTalantapt_PT
oaire.citation.volume222pt_PT
person.familyNameGonçalves de Barros
person.familyNameSantos
person.givenNamePiedade Aurora
person.givenNameMarlene
person.identifier1508370
person.identifier.ciencia-idD210-6003-454B
person.identifier.ciencia-id8311-B967-31C4
person.identifier.orcid0000-0002-2635-251X
person.identifier.orcid0000-0001-5020-5942
person.identifier.scopus-author-id23981959400
person.identifier.scopus-author-id57110502000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationa85b368a-8f13-45eb-a744-7c5810a38e2c
relation.isAuthorOfPublication8ce9ee39-a4c6-46ae-99e2-49397b550f1b
relation.isAuthorOfPublication.latestForDiscoverya85b368a-8f13-45eb-a744-7c5810a38e2c

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