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Advisor(s)
Abstract(s)
This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic
dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting
A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a
A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the
selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich
format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a oncentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD <5.2% was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean.
Description
Keywords
Alexandrium minutum Dinoflagellate Chronoamperometry Electrochemical genosensor Sandwich format hybridization
Pedagogical Context
Citation
Morais, S. L., Barros, P., Santos, M., Delerue-Matos, C., Gomes, A. C., & Fátima Barroso, M. (2021). Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta, 222, 121416. https://doi.org/10.1016/j.talanta.2020.121416
Publisher
Elsevier