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Phytosome-based nanocarriers enhanced with seaweed extracts: Overcoming the blood–brain barrier

2025-06-10Conference paper Open access
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dc.contributor.authorPortela, Mariana
dc.contributor.authorSilva, Aurora
dc.contributor.authorCarpena, Maria
dc.contributor.authorGrosso, Clara
dc.contributor.authorBarroso, Maria Fátima
dc.contributor.authorOliveira, Ana Isabel
dc.contributor.authorMartins, Claudia
dc.contributor.authorRibeiro, Cristina
dc.contributor.authorPrieto, Miguel A.
dc.contributor.authorOliveira, Ana Isabel
dc.date.accessioned2025-11-21T16:36:17Z
dc.date.available2025-11-21T16:36:17Z
dc.date.issued2025-06-10
dc.description.abstractNeurodegenerative diseases impact millions of people globally and are emerging as an imminent challenge due to the rapid aging of the population. The current treatments only focus on relieving their symptoms, so it is necessary to adopt innovative strategies. However, delivering pharmacological agents directly into the brain is difficult because of the presence of the Blood–Brain Barrier (BBB). To overcome this obstacle, nanotransporters such as phytosomes have been developed. This study reports the preparation and characterization of phosphatidylcholine (PC) phytosomes based on hydroethanolic extracts of three macroalgae: Ascophyllum nodosum (L.) Le Jolis (AN), Bifurcaria bifurcata R.Ross (BB), and Fucus spiralis L. (FS). Additionally, some phytosomes were functionalized with polyethylene glycol (PEG) and apolipoprotein E (ApoE). Phytosome characterization was carried out in terms of encapsulation rate, size, polydispersity index (PDI), zeta potential, and stability, and the efficacy of passage through the BBB was tested using an in vitro transwell model based on hCMEC/D3 cells. The results showed a high percentage of extract bound to PC (from 74.9 to 80.3%), and tests conducted over three weeks showed the stability of the phytosomes developed. There was a notable distinction between the functionalized and non-functionalized phytosomes, reflected in the values of their sizes (from 117.71 to 167.73 nm for non-functionalized and from 277.07 to 361.44 nm for PEG-ApoE phytosomes), PDIs (0.286–0.411 for non-functionalized and 0.389-0.539 for functionalized phytosomes), and zeta potentials (1.91–2.22 and −3.31–−0.68 mV for non-functionalized and functionalized phytosomes), respectively. Regarding their ability to cross the BBB, the functionalization of phytosomes with ApoE did not prove to be a crucial step, perhaps due to the low amount of ApoE used (1%), as all of the nanotransporters always passed through the hCMEC/D3 cell monolayer, regardless of their formulation.por
dc.description.sponsorshipCYTED—GENOPSYSEN, P222RT0117
dc.identifier.citationPortela, M., Silva, A., Carpena, M., Grosso, C., Barroso, M. F., Oliveira, A. I., Martins, C., Ribeiro, C., & Prieto, M. A. (2025). Phytosome-based nanocarriers enhanced with seaweed extracts: Overcoming the blood–brain barrier. Engineering Proceedings - 5th International Electronic Conference on Applied Sciences, 87(1), 75. https://doi.org/10.3390/engproc2025087075
dc.identifier.doi10.3390/engproc2025087075
dc.identifier.urihttp://hdl.handle.net/10400.22/31028
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationUID/50006
dc.relation.hasversionhttps://www.mdpi.com/2673-4591/87/1/75
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBlood–brain barrier
dc.subjectPhytosomes
dc.subjectMacroalgae
dc.titlePhytosome-based nanocarriers enhanced with seaweed extracts: Overcoming the blood–brain barrierpor
dc.typeconference paper
dspace.entity.typePublication
oaire.citation.conferenceDate2024-12
oaire.citation.issue1
oaire.citation.titleEngineering Proceedings - 5th International Electronic Conference on Applied Sciences
oaire.citation.volume87
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameOliveira
person.givenNameAna Isabel
person.identifier.ciencia-id9212-F501-E9EF
person.identifier.orcid0000-0003-4420-6476
relation.isAuthorOfPublication4b3fe293-2740-469f-97d3-99d0b107b5e0
relation.isAuthorOfPublication.latestForDiscovery4b3fe293-2740-469f-97d3-99d0b107b5e0

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