Publication
Food allergen control: Tropomyosin analysis through electrochemical immunosensing
| dc.contributor.author | Torre, Ricarda | |
| dc.contributor.author | Freitas, Maria | |
| dc.contributor.author | Costa‐Rama, Estefanía | |
| dc.contributor.author | Nouws, Henri | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.date.accessioned | 2023-01-25T14:55:42Z | |
| dc.date.available | 2023-01-25T14:55:42Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Regulations of the EU obliges the indication of the presence of allergens on food labels. This work reports the development of an electrochemical immunosensor to determine tropomyosin (TPM) – a major shellfish allergen – prevailing in the muscles of crustacean species. Two linear ranges between the signal and TPM concentration were obtained: between 2.5 and 20 ng mL−1 and between 30 and 200 ng mL−1, with a lowest limit of detection of 0.47 ng mL−1. The selectivity of the optimized immunoassay, tested with other food allergens (e.g., Cyp c 1, a fish allergen), assures the effective detection of TPM, enabling successful control of foodstuff labelling. Several (12) foods, containing high and low TPM concentrations and TPM-free samples, were analysed using the sensor. A conventional ELISA kit and recovery assays were used to evaluate the accuracy of the results. | pt_PT |
| dc.description.sponsorship | This work was financially supported by Portuguese national funds through projects UIDB/50006/2020, UIDP/50006/2020, and LA/P/ 0008/2020, from the Fundação para a Ciência e a Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES). The authors also thank FCT and the EU for funding through the project PTDC/ QUI-QAN/30735/2017—TracAllerSens—Electrochemical sensors for the detection and quantification of trace amounts of allergens in food products (POCI-01-0145-FEDER-030735), supported by national funds by FCT/MCTES and co-supported by Fundo Europeu de Desenvolvimento Regional (FEDER) through COMPETE 2020—Programa Operacional Competitividade e Internacionalização. E. Costa-Rama thanks the support of the grant “Beatriz Galindo” (BG20/00027) funded by the Ministerio de Universidades of the Spanish Government. R. Torre thanks FCT and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for her PhD grant (SFRH/BD/143753/2019). The authors are thankful to Rui Rocha and CEMUP for the SEM/EDS work. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.foodchem.2022.133659 | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.22/21860 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | LA/P/ 0008/2020 | pt_PT |
| dc.relation | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| dc.relation | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| dc.relation | PTDC/ QUI-QAN/30735/2017 | |
| dc.relation | Electrochemical biosensors for analysis of safety and quality parameters in fish and processed fishery products | |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0308814622016211?via%3Dihub | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Shellfish | pt_PT |
| dc.subject | Crustacean | pt_PT |
| dc.subject | Food allergy | pt_PT |
| dc.subject | Tropomyosin | pt_PT |
| dc.subject | Immunosensor | pt_PT |
| dc.subject | Electrochemical biosensor | pt_PT |
| dc.title | Food allergen control: Tropomyosin analysis through electrochemical immunosensing | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| oaire.awardTitle | Associated Laboratory for Green Chemistry - Clean Technologies and Processes | |
| oaire.awardTitle | Electrochemical biosensors for analysis of safety and quality parameters in fish and processed fishery products | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F143753%2F2019/PT | |
| oaire.citation.startPage | 133659 | pt_PT |
| oaire.citation.title | Food Chemistry | pt_PT |
| oaire.citation.volume | 396 | pt_PT |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | POR_NORTE | |
| person.familyName | Torre | |
| person.familyName | Nouws | |
| person.familyName | Delerue-Matos | |
| person.givenName | Ricarda | |
| person.givenName | Hendrikus | |
| person.givenName | Cristina | |
| person.identifier | 1046746 | |
| person.identifier.ciencia-id | 2015-9883-AD97 | |
| person.identifier.ciencia-id | BC12-319B-037F | |
| person.identifier.ciencia-id | 9B1A-9CED-6EE3 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0003-2843-8402 | |
| person.identifier.orcid | 0000-0002-0628-2753 | |
| person.identifier.orcid | 0000-0002-1472-8866 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | D-5249-2013 | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 7801450247 | |
| person.identifier.scopus-author-id | 6603741848 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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