Publication
The effect of synthetic cannabinoid ADB-FUBI-NACA on primary neuronal cultures ß-galacto-sidase activity: preliminary findings
| dc.contributor.author | Roque-Bravo, Rita | |
| dc.contributor.author | Carmo, Helena | |
| dc.contributor.author | Silva, João Pedro | |
| dc.contributor.author | Carvalho, Félix | |
| dc.contributor.author | Silva, Diana Dias da | |
| dc.contributor.author | Dias da Silva, Diana Cristina | |
| dc.date.accessioned | 2025-10-28T14:48:43Z | |
| dc.date.available | 2025-10-28T14:48:43Z | |
| dc.date.issued | 2024-05-01 | |
| dc.description.abstract | ADB-FUBINACA (ADB-FUB) is a synthetic cannabinoid (SC) that has gained popularity among users as a new psychoactive substance. This stems from SC's pharmacological similarity to the active principle of cannabis, D9-tetrahydrocannabinol (THC). However, unlike THC, SCs demonstrate full agonism of cannabinoid receptors 1 and 2 [1]. Recent scientific developments have shown that can-nabis use may aggravate ageing-related parameters [2,3]. Moreover, a study using human fibroblasts re-vealed that 1 μM THC (2h-longexposure, for 15 days) can increase ß-galactosidase activity [3], which serves as a first-line marker for cellular senescence. This study was designed to investigate whether these biologically-relevant concentrations could accelerate neuronal ageing. PHC were isolated from Wistar rat day 18-19 embryos and cultured for up to 21 days-in-vitro (DIV). Exposure to 1 pM, 1 nM and 1 μM ADB-FUB (concentrations previously shown to be non-cytotoxic to PHC) started either on DIV3 or DIV7 and was maintained up to 21 DIV. At that final timepoint, ß-galactosidase activity was evaluated. DMSO at 0.02% was employed as solvent control. Under these experi-mental conditions, PHC exposed to 1 nM and 1 μM ADB-FUB in the DIV3-21 protocol had lower ß-galactosidase activity when compared to control conditions (p<0.05, 1 nM; p<0.001, 1 μM). No statisti-cally significant results were registered for PHC under the DIV7-21 exposure protocol. These findings are, to the best of our knowledge, the first evidence of a potential “anti-ageing” effect of ADB-FUB. Evaluation of other senescence-related endpoints will follow. Moreover, experiments using another in vitroneuronal model (human neuroblastoma cell line SH-SY5Y) are underway to compare the effects of the same drug in different models and further substantiate conclusions on ADB-FUB’s effect. | por |
| dc.identifier.citation | Roque-Bravo, R., Carmo, H., Silva, J. P., Carvalho, F., & Dias-da-Silva, D. (2024). The effect of synthetic cannabinoid ADB-FUBINACA on primary neuronal cultures ß-galactosidase activity: Preliminary findings. Scientific Letters, 1(Sup 1). https://doi.org/10.48797/sl.2024.135 | |
| dc.identifier.doi | 10.48797/sl.2024.135 | |
| dc.identifier.issn | 2795-5117 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/30698 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | IUCS-CESPU Publishing | |
| dc.relation | UIDP/04378/2020; UIDB/04378/2020 | |
| dc.relation.hasversion | https://publicacoes.cespu.pt/index.php/sl/article/view/135 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Synthetic cannabinoids | |
| dc.subject | In vitroneurotoxicity | |
| dc.subject | Ageing | |
| dc.title | The effect of synthetic cannabinoid ADB-FUBI-NACA on primary neuronal cultures ß-galacto-sidase activity: preliminary findings | por |
| dc.type | conference paper | |
| dspace.entity.type | Publication | |
| oaire.citation.conferenceDate | 2024-05 | |
| oaire.citation.conferencePlace | Porto | |
| oaire.citation.issue | Sup1 | |
| oaire.citation.volume | 1 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Dias da Silva | |
| person.givenName | Diana Cristina | |
| person.identifier.ciencia-id | 7715-CF06-F0B5 | |
| person.identifier.orcid | 0000-0002-7331-9157 | |
| relation.isAuthorOfPublication | 1f32faf1-efa5-4ea0-982d-a755e1940abf | |
| relation.isAuthorOfPublication.latestForDiscovery | 1f32faf1-efa5-4ea0-982d-a755e1940abf |