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Advisor(s)
Abstract(s)
The present work aimed to study an eventual antimicrobial resistance and the presence of β-lactamases in enterobacteria isolated from cheese. β-lactams are among the most common antibiotics used for bacterial infections. Rates of bacterial resistance to antimicrobial agents are increasing world wide and the presence of β-lactamases is among one of the most common resistance mechanisms. In the present study we used twenty cheese samples, corresponding to six different types of Portuguese cheese, collected from local retail establishments. Samples were screened for the presence of β-lactams resistant Enterobacteriaceae in selective media with high concentration values of ampicilin. Colonies presumed to be resistant to ampicilin were further confirmed by antibiogram testing against other 13 different antibiotics, including six β-lactams. A total of 160 isolates were recovere from the selective media. The presence of β-lactamases on colonies was detected by adding nitrocefin to the cell biomass. After antibiogram testing about 40 isolates were confirmed to be resistant to, at least, one β -lactam subclass. The results obtained in this study point out to the presence, in different cheese samples, of Enterobacteriaceae resistant to β-lactams and the presence of β-lactamases. These results suggest that cheese may be a source of potential resistance transfer to the normal gastrointestinal flora of the consumer,which may be a cause of serious public health problems.
Description
Keywords
Antimicrobial resistance β-lactamases
Citation
Amador, P., Fernandes, R., Tato-Costa, J., Teles-Grilo, L., Brito, L., & Prudêncio, C. (2007). Antimicrobial resistance in bacterial strains isolated from different types of Portuguese cheese. Livro de actas do congresso MICRO’07 – BIOTEC’07 – XXXIII JPG, 38.
Publisher
Escola Superior de Biotecnologia, Universidade Católica Portuguesa