Oxidative stress genes involved in the virulence-dependent susceptibility to antibiotics in Pseudomonas aeruginosa

Coelho, Pedro Barata; Fernandes, Ruben; Silva, Carina; Oliveira, Marco; Veiga, Marlene; Sá, Sara; Vieira, André; Guedes, Carla; Baylina, Pilar

http://hdl.handle.net/10400.22/26244

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Abstract(s)

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen which rarely causes disease in healthy people. P. aeruginosa, in particular strain PAO1 is also a biological model for studying virulence and bacterial social traits, such as quorum sensing, SOS response among other. Antibiotic response is dependent, among several other factors, to the response to environmental stress conditions. The present study aims to understand the role of 10 PAO1 oxidative gene mutants in the response to antibiotic stress in elastase, protease and pyocyanin-dependent virulence factors. PAO1 was stressed to several antibiotics (penicilins, cephalosporins, macrolides, and quinolones), and the virulence proteins were measured by means of spectroscopic methods. Viability was measured by means of Erythrosin B. PAO1 GGT, GLO1, RubA2, GSH A mutants were the most susceptible to the production of virulence-dependent factors.

Keywords

Pseudomonas aeruginosa Pathogen

URI

http://hdl.handle.net/10400.22/26244

Citation

Coelho, P. B., Fernandes, R., Silva, C., Oliveira, M., Veiga, M., Sá, S., Vieira, A., Guedes, C., & Baylina, P. (2021). Oxidative stress genes involved in the virulence-dependent susceptibility to antibiotics in Pseudomonas aeruginosa. Livro de resumos XXVIII Congreso Nacional de Microbiología, 209.