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Quantification of tranexamic acid in human plasma: development and validation of UHPLC-MS/MS method

2018-03Conference poster Open access
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dc.contributor.authorBarreiros, Luisa
dc.contributor.authorAmoreira, Júlia L.
dc.contributor.authorMachado, Sandia
dc.contributor.authorFernandes, Sara R.
dc.contributor.authorSilva, Eduarda M. P.
dc.contributor.authorSá, Paula
dc.contributor.authorKietaibl, Sibylle
dc.contributor.authorSegundo, Marcela A.
dc.contributor.authorFernandes, Sara
dc.date.accessioned2025-07-08T08:45:06Z
dc.date.available2025-07-08T08:45:06Z
dc.date.issued2018-03
dc.description.abstractTranexamic acid (TXA), an antifibrinolytic drug with the ability to inhibit lysine binding at plasminogen receptors, can be used in different settings such as trauma, cardiac surgery, major orthopedic surgery, obstetric when perioperative bleeding is concerned [1]. Effective methods for determination of TXA in biological samples are still required to understand the pharmacokinetics and pharmacodynamics of this drug in variable age groups undergoing surgeries with high blood loss [2]. The development and validation of a method based on ultra-high performance liquid chromatography coupled to triple quadrupole-tandem mass spectrometry (UHPLCMS/MS) to quantify TXA in human plasma is described herein. A simple, inexpensive and efficient sample treatment involving protein precipitation with acetonitrile containing 0.5% (v/v) formic acid was implemented using volumes within the microliter range. Separation was achieved using a hydrophilic interaction based stationary phase and ammonium bicarbonate in the mobile phase that permitted a more efficient separation of the analyte from the matrix interferences, thus reducing matrix effects and increasing method sensitivity. The method was validated according to the European Medicines Agency guideline [3]. Excellent linearity was achieved (r2 > 0.997) for TXA concentrations ranging from 30 to 600 ng mL-1 with LOD and LOQ of 3 and 6 ng mL-1 in plasma extracts, respectively. The developed method proved to be selective, sensitive, accurate (96.4-105.7% of nominal concentration values) and precise (RSD ≤ 4.5%).por
dc.description.sponsorshipNORTE-01-0145-FEDER-000011
dc.identifier.citationBarreiros, L., Amoreira, J. L., Machado, S., Fernandes, S. R., Silva, E. M. P., Sá, P., Kietaibl, S., & Segundo, M. A. (2018). Quantification of tranexamic acid in human plasma: Development and validation of UHPLC-MS/MS method. Book of Abstracts Analítica 2018 - 9th Meeting of Division of Analytical Chemistry, 80. https://analitica2018.eventos.chemistry.pt/images/book.pdf
dc.identifier.isbn978-989-8124-21-0
dc.identifier.urihttp://hdl.handle.net/10400.22/30212
dc.language.isoeng
dc.peerreviewedn/a
dc.publisherSociedade Portuguesa de Química
dc.relationPT2020 UID/QUI/50006/2013 - POCI/01/0145/FEDER/007265
dc.relation.hasversionhttps://analitica2018.eventos.chemistry.pt/images/book.pdf
dc.rights.uriN/A
dc.subjectTranexamic acid
dc.titleQuantification of tranexamic acid in human plasma: development and validation of UHPLC-MS/MS methodpor
dc.typeconference poster
dspace.entity.typePublication
oaire.citation.conferenceDate2018-03
oaire.citation.conferencePlaceFFUP/ICBAS – UNIVERSITY OF PORTO
oaire.citation.startPage80
oaire.citation.titleBook of Abstracts Analítica 2018 - 9th Meeting of Division of Analytical Chemistry
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameBarreiros
person.familyNameFernandes
person.givenNameLuisa
person.givenNameSara
person.identifier.ciencia-id611F-E0C5-0230
person.identifier.ciencia-id1C12-D800-38A4
person.identifier.orcid0000-0003-3481-5809
person.identifier.orcid0000-0001-7042-1941
person.identifier.ridD-7950-2013
person.identifier.scopus-author-id6508205485
person.identifier.scopus-author-id57203278917
relation.isAuthorOfPublication1e66bacc-64de-4ecb-96b7-4c0e366cba57
relation.isAuthorOfPublication6c2ed5f0-c3fd-4559-99a3-b8d78d019b47
relation.isAuthorOfPublication.latestForDiscovery1e66bacc-64de-4ecb-96b7-4c0e366cba57

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