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Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli

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dc.contributor.authorMarani, Mariela M.
dc.contributor.authorPerez, Luis O.
dc.contributor.authorde Araujo, Alyne Rodrigues
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorSousa, Carla F.
dc.contributor.authorQuelemes, Patrick Veras
dc.contributor.authorOliveira, Mayara
dc.contributor.authorGomes-Alves, Ana G.
dc.contributor.authorPueta, Mariana
dc.contributor.authorGameiro, Paula
dc.contributor.authorTomás, Ana M.
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorEaton, Peter
dc.contributor.authorCamperi, Silvia A.
dc.contributor.authorBasso, Néstor G.
dc.contributor.authorLeite, José Roberto de Souza de Almeida
dc.date.accessioned2019-05-30T14:46:52Z
dc.date.available2019-05-30T14:46:52Z
dc.date.issued2017
dc.description.abstractPatagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now. In this sense, Pleurodema thaul is the first amphibian specie to be studied from this large region of South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobial activity against Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic, Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7μM). AFM and SPR studies suggested a preferential interaction between these peptides and bacterial membranes. Cytotoxicity assays showed that thaulin peptides had minimal effects at MIC concentrations towards human and animal cells. These are the first peptides described for amphibians of the Pleurodema genus. These findings highlight the potential of the Patagonian region's unexplored biodiversity as a source for new molecule discovery.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.gene.2016.12.020pt_PT
dc.identifier.issn0378-1119
dc.identifier.urihttp://hdl.handle.net/10400.22/13812
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-07-0124-FEDER-000002-Host-Pathogen Interactionspt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0378111916310204?via%3Dihubpt_PT
dc.subjectAmino Acid Sequencept_PT
dc.subjectAmphibian Proteinspt_PT
dc.subjectAnimalspt_PT
dc.subjectAntimicrobial Cationic Peptidespt_PT
dc.subjectAnurapt_PT
dc.subjectBase Sequencept_PT
dc.subjectCell Survivalpt_PT
dc.subjectDNA, Complementarypt_PT
dc.subjectErythrocytespt_PT
dc.subjectEscherichia colipt_PT
dc.subjectGene Expressionpt_PT
dc.subjectKlebsiella pneumoniaept_PT
dc.subjectMacrophagespt_PT
dc.subjectMicept_PT
dc.subjectMice, Inbred BALB Cpt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectModels, Molecularpt_PT
dc.subjectProtein Structure, Secondarypt_PT
dc.subjectSequence Alignmentpt_PT
dc.subjectSkinpt_PT
dc.subjectSolid-Phase Synthesis Techniquespt_PT
dc.subjectStaphylococcus aureuspt_PT
dc.titleThaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia colipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F97995%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F93766%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.citation.endPage80pt_PT
oaire.citation.startPage70pt_PT
oaire.citation.titleGenept_PT
oaire.citation.volume605pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStream5876
person.familyNameDelerue-Matos
person.givenNameCristina
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id6603741848
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isProjectOfPublicationdf750fb0-a21e-43b2-8a67-f8607fefefcf
relation.isProjectOfPublication9d89744e-cf85-4e92-ba5d-8a6fb11596fb
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