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Contributo das técnicas histoquímicas no diagnóstico veterinário - estudo casuístico de 10 anos no INIAV (Polo de Vairão)
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A Patologia Veterinária estuda as doenças dos animais domésticos, silvestres, zoológicos e de produção. O exame histopatológico faz parte da rotina de um laboratório de Patologia, recorrendo-se a técnicas histoquímicas para esclarecimento do diagnóstico. Este estudo foi realizado no Laboratório de Patologia do Laboratório de Veterinária de Vairão-INIAV onde não havia um levantamento descritivo acerca das técnicas histoquímicas que foram realizadas e qual o seu contributo para o diagnóstico. Assim, este trabalho teve como objetivo reunir e analisar informação das técnicas histoquímicas realizadas entre 2014-2023 neste laboratório e avaliar o seu contributo. Obtiveram-se os dados dos registos técnicos relativos às histoquímicas, que foram organizados e distribuídos em 4 grupos de animais: companhia, produção, exóticos e silvestres. A grande maioria das amostras pertencia a caninos, felinos e bovinos. Para caninos e felinos as principais colorações foram as de Azul toluidina para patologias inflamatórias e mastocitomas e as de Ácido periódico de Schiff, Grocott e Ziehl-Neelsen para deteção de microrganismos. Para bovinos, Ziehl-Neelsen foi necessário para o diagnóstico de tuberculose. O contributo associado às técnicas histoquímicas realizadas no presente estudo, permitiu demonstrar a relevância destas colorações como ferramentas complementares no diagnóstico em Patologia Veterinária.
Veterinary Pathology studies the diseases of domestic, wild, zoo, and production animals. Histopathological examination is part of the routine in a Pathology laboratory, making use of histochemical techniques to aid in diagnosis. This study was carried out at the Pathology Laboratory of the Veterinary Laboratory of Vairão-INIAV, where no descriptive survey had previously been conducted regarding the histochemical techniques used and their contribution to diagnosis. Therefore, the aim of this work was to collect and analyze information on the histochemical techniques performed between 2014 and 2023 in this laboratory and to evaluate their contribution. Data was obtained from technical records related to histochemical procedures and then organized and grouped into four categories of animals: companion, production, exotic, and wild animals. Most of the samples came from canines, felines, and bovines. For dogs and cats, the main stains used were Toluidine Blue for inflammatory pathologies and mast cell tumors, and Periodic Acid–Schiff, Grocott, and Ziehl-Neelsen for the detection of microorganisms. For cattle, Ziehl-Neelsen was essential for the diagnosis of tuberculosis. The contribution associated with the histochemical techniques performed in the present study demonstrates the relevance of these stains as complementary tools in the diagnosis of Veterinary Pathology.
Veterinary Pathology studies the diseases of domestic, wild, zoo, and production animals. Histopathological examination is part of the routine in a Pathology laboratory, making use of histochemical techniques to aid in diagnosis. This study was carried out at the Pathology Laboratory of the Veterinary Laboratory of Vairão-INIAV, where no descriptive survey had previously been conducted regarding the histochemical techniques used and their contribution to diagnosis. Therefore, the aim of this work was to collect and analyze information on the histochemical techniques performed between 2014 and 2023 in this laboratory and to evaluate their contribution. Data was obtained from technical records related to histochemical procedures and then organized and grouped into four categories of animals: companion, production, exotic, and wild animals. Most of the samples came from canines, felines, and bovines. For dogs and cats, the main stains used were Toluidine Blue for inflammatory pathologies and mast cell tumors, and Periodic Acid–Schiff, Grocott, and Ziehl-Neelsen for the detection of microorganisms. For cattle, Ziehl-Neelsen was essential for the diagnosis of tuberculosis. The contribution associated with the histochemical techniques performed in the present study demonstrates the relevance of these stains as complementary tools in the diagnosis of Veterinary Pathology.
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Veterinária Patologia Histoquímica Estudo casuístico