Publication
Possible modulation of dopaminergic neurotransmission function by acetyl-L-carnitine
| dc.contributor.author | Cunha, L. | |
| dc.contributor.author | Szigeti, K. | |
| dc.contributor.author | Mathe, D. | |
| dc.contributor.author | Summavielle, T. | |
| dc.contributor.author | Metello, L. | |
| dc.date.accessioned | 2023-05-23T15:46:39Z | |
| dc.date.available | 2023-05-23T15:46:39Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Acetyl‐L‐Carnitine (ALC) has a putative neuroprotective effect being used in a variety of conditions. Nevertheless, the underlying molecular mechanisms, particularly regarding the induction of changes in neurotransmitter systems, are still not fully understood. We aim to contribute for the elucidation of the mechanisms by which ALC alters neurotransmitter release, using a cell line and an animal model of exposure to methamphetamine (METH). PC12 cells were incubated with several doses of ALC (0.01 to 1.0 mM) alone or in combination with METH 1.0 or 100 µM for 24h or 72h. When combined, ALC preceded METH administration in 30 minutes. Dopamine (DA) content was determined by high performance liquid chromatography. C57BL/6J mice were used for in vivo assays to assess DA striatal binding. Mice were divided into 4 groups, according to different treatments: group 1 (control), group 2 (ALC, 100 mg/kg), group 3 (METH, 10 mg/kg) and group 4 (ALC+METH). Images were acquired in a SPECT/CT scanner (NanoSPECT/CT, Mediso, Hungary) 70 minutes after 123I‐IBZM injection. Regions of interest were drawn in the striata and in the cerebellum to determine the striatal binding ratio. Increased intracellular levels of DA were observed in PC12 cells at 24h and 72h after the administration of ALC. Cells treated with METH 100 µM displayed decreased intracellular levels of DA. ALC prevented the METH‐induced decrease in DA concentration (p<0.0001). On the other hand, a single dose of 10 mg/kg of METH induced a decrease in striatal D2R binding ratios comparing to control group (between 20% and 30%). Interestingly, over time, ALC was able to reverse the decrease on the radiotracer binding induced by METH. The present study indicates a possible effect of ALC over METH‐induced DA release. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | L. Cunha, K. Szigeti, D. Mathe, T. Sumavielle, & L. Metello. (2014). Possible modulation of dopaminergic neurotransmission function by acetyl-L-carnitine. EANM’14, Annual Congress of the European Association of Nuclear Medicine, 41 (2): S151-S705, S626. | pt_PT |
| dc.identifier.eissn | 1619-7089 | |
| dc.identifier.issn | 1619-7070 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/22903 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Springer | pt_PT |
| dc.relation.publisherversion | https://link.springer.com/journal/259/volumes-and-issues/41-2/supplement | pt_PT |
| dc.title | Possible modulation of dopaminergic neurotransmission function by acetyl-L-carnitine | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | S626 | pt_PT |
| oaire.citation.title | European Journal of Nuclear Medicine and Molecular Imaging | pt_PT |
| oaire.citation.volume | 41 (Suppl 2):S151–S705 | pt_PT |
| person.familyName | Summavielle | |
| person.givenName | Teresa | |
| person.identifier | 677706 | |
| person.identifier.ciencia-id | C41E-0816-5C85 | |
| person.identifier.orcid | 0000-0003-2548-6281 | |
| person.identifier.rid | C-9776-2012 | |
| person.identifier.scopus-author-id | 6603092949 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | 207ee2de-85a0-4144-9e7e-b376c600e065 | |
| relation.isAuthorOfPublication.latestForDiscovery | 207ee2de-85a0-4144-9e7e-b376c600e065 |