Repository logo
 
Publication

VKORC1 gene polymorphism as cardiovascular biomarker: Detection by electrochemical genosensors

2022-05Conference object Open access
Simple item page
dc.contributor.authorCosta, Inês
dc.contributor.authorJ. S. F. Sousa, António
dc.contributor.authorBanegas, Rodrigo S.
dc.contributor.authorMorais, Stephanie L.
dc.contributor.authorMagalhães, Júlia
dc.contributor.authorRodrigues, Valentina
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorRamos-Jesus, Joilson
dc.contributor.authorFerreira-Fernandes, Hygor
dc.contributor.authorPinto, Giovanny R.
dc.contributor.authorSantos, Marlene
dc.contributor.authorBarroso, M. Fátima
dc.date.accessioned2023-09-14T15:24:25Z
dc.date.available2023-09-14T15:24:25Z
dc.date.issued2022-05
dc.description.abstractWarfarin is an anticoagulant generally used to prevent cardiovascular diseases. Since of the low therapeutic index of warfarin and frequent complications of prevention or treatment, significant differences in individual doses of warfarin are needed to achieve prophylactic and therapeutic ranges. Recent studies have been reporting that genetic variants of vitamin K epoxide reductase complex (VKORC1) influence the response to warfarin and doses [9]. So, the genetic and pharmacogenetic information of the major cardiovascular diseases plays an important role in the identification of the cardiovascular risk factors and in the diagnosis and treatment of these conditions. This work addresses the development of a disposable electrochemical genosensor able of detecting single nucleotide polymorphism (SNP) in the VKORC1 gene. Analysing public databases, two specific 52 bp DNA probes, one with adenine (TA) and another with guanine (TG) SNP genetic variation were selected and selected and designed. The genosensor methodology implied the immobilization of a mixed self-assembled monolayer (SAM) linear VKORC1 DNA-capture probe and mercaptohexanol (MCH) onto screen-printed gold electrodes (SPGE). To improve the genosensor´s selectivity and avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the VKORC1 allele was designed using a complementary fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Preliminary studies indicate that differences in the electrochemical answers were obtained depending of the hybridization reaction format. In fact, higher electrochemical intensities were measured when the hybridization reaction was performed with a complementary DNA (without SNPs). These results suggested that the sensor is able to discriminate between the complementary DNA and single base mismatch targets having a great potential for the DNA polymorphism analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCosta, I., Sousa, A. J. S. F., Banegas, R. S., Morais, S. L., Magalhães, J., Rodrigues, V., Delerue-Matos, C., Ramos-Jesus, J., Ferreira-Fernandes, H., R. Pinto, G., Santos, M., & Barroso, M. F. (2022, maio). VKORC1 gene polymorphism as cardiovascular biomarker: Detection by electrochemical genosensors [Poster]. 15º Encontro de Investigação Jovem da U.Porto, Portopt_PT
dc.identifier.isbn978-989-746-329-7
dc.identifier.urihttp://hdl.handle.net/10400.22/23534
dc.language.isoengpt_PT
dc.publisherUniversidade do Portopt_PT
dc.relation.publisherversionhttps://www.up.pt/ijup/wp-content/uploads/sites/892/2023/05/Livro_de_Resumos_versao_final-rev2.pdfpt_PT
dc.titleVKORC1 gene polymorphism as cardiovascular biomarker: Detection by electrochemical genosensorspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
oaire.citation.endPage510pt_PT
oaire.citation.startPage509pt_PT
oaire.citation.titleLivro de Resumos do 15.º Encontro de Investigação Jovem da U.Portopt_PT
person.familyNameSantos
person.givenNameMarlene
person.identifier1508370
person.identifier.ciencia-id8311-B967-31C4
person.identifier.orcid0000-0001-5020-5942
person.identifier.scopus-author-id57110502000
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication8ce9ee39-a4c6-46ae-99e2-49397b550f1b
relation.isAuthorOfPublication.latestForDiscovery8ce9ee39-a4c6-46ae-99e2-49397b550f1b

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
POSTER_Marlene Santos 1.pdf
Size:
86.4 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ESS - CISA - Posters apresentados em eventos científicos
ESS - FAR - Posters apresentados em eventos científicos