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Advisor(s)
Abstract(s)
Given the pandemic situation, there is an
urgent need for an accurate test to monitor antibodies
anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution
of coronavirus disease in 2019 (COVID-19) and to
stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulosebased test strip for rapid and cost-efective quantitative detection of antibodies to SARS-CoV2 virus by
colorimetric transduction. For this purpose, Whatman paper was chemically modifed with sodium
metaperiodate to introduce aldehyde groups on its
surface. Subsequently, the spike protein of the virus
is covalently bound by forming an imine group. The chemical control of cellulose paper modifcation was
evaluated by Fourier transform infrared spectroscopy,
thermogravimetry and contact angle analysis. Colorimetric detection of the antibodies was performed by a
conventional staining method using Ponceau S solution as the dye. Color analysis was performed after
image acquisition with a smartphone using Image J
software. The color intensity varied linearly with the
logarithm of the anti-S concentration (from 10 ng/mL
to 1 μg/mL) in 500-fold diluted serum samples when
plotted against the green coordinate extracted from
digital images. The test strip was selective in the presence of nucleocapsid antibodies, urea, glucose, and
bovine serum albumin with less than 15% interference, and detection of antibodies in human serum was
successfully performed. Overall, this is a simple and
afordable design that can be readily used for mass
Abstract Given the pandemic situation, there is an population screening and does not require sophisticated equipment or qualifed personnel
Description
Keywords
Colorimetric Dye Protein spike Serum Test-strips
Citation
Publisher
Springer