Repository logo
 
Publication

Development of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 virus

2022Journal article Restricted access
Simple item page
dc.contributor.authorCorreia, Bárbara P.
dc.contributor.authorSousa, Mariana P.
dc.contributor.authorSousa, Cristina E. A.
dc.contributor.authorMateus, Daniela
dc.contributor.authorSebastião, Ana Isabel
dc.contributor.authorCruz, Maria Teresa
dc.contributor.authorMatos, Ana Miguel
dc.contributor.authorSantos Pereira, Ana Cláudia
dc.contributor.authorMoreira, Felismina
dc.date.accessioned2023-01-26T09:05:57Z
dc.date.embargo2035
dc.date.issued2022
dc.description.abstractGiven the pandemic situation, there is an urgent need for an accurate test to monitor antibodies anti-SARS-CoV-2, providing crucial epidemiological and clinical information to monitor the evolution of coronavirus disease in 2019 (COVID-19) and to stratify the immunized and asymptomatic population. Therefore, this paper describes a new cellulosebased test strip for rapid and cost-efective quantitative detection of antibodies to SARS-CoV2 virus by colorimetric transduction. For this purpose, Whatman paper was chemically modifed with sodium metaperiodate to introduce aldehyde groups on its surface. Subsequently, the spike protein of the virus is covalently bound by forming an imine group. The chemical control of cellulose paper modifcation was evaluated by Fourier transform infrared spectroscopy, thermogravimetry and contact angle analysis. Colorimetric detection of the antibodies was performed by a conventional staining method using Ponceau S solution as the dye. Color analysis was performed after image acquisition with a smartphone using Image J software. The color intensity varied linearly with the logarithm of the anti-S concentration (from 10 ng/mL to 1 μg/mL) in 500-fold diluted serum samples when plotted against the green coordinate extracted from digital images. The test strip was selective in the presence of nucleocapsid antibodies, urea, glucose, and bovine serum albumin with less than 15% interference, and detection of antibodies in human serum was successfully performed. Overall, this is a simple and afordable design that can be readily used for mass Abstract Given the pandemic situation, there is an population screening and does not require sophisticated equipment or qualifed personnelpt_PT
dc.description.sponsorshipThis project was a fnancially supported through the project, TecniCov (POCI-01-02B7-FEDER-069745), cofunded by FEDER through COMPETE2020 and Lisboa2020.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s10570-022-04808-ypt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/21874
dc.language.isoengpt_PT
dc.publisherSpringerpt_PT
dc.relationPOCI-01-02B7-FEDER-069745pt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10570-022-04808-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectColorimetricpt_PT
dc.subjectDyept_PT
dc.subjectProtein spikept_PT
dc.subjectSerumpt_PT
dc.subjectTest-stripspt_PT
dc.titleDevelopment of colorimetric cellulose-based test-strip for the rapid detection of antibodies against SARS-CoV2 viruspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9322pt_PT
oaire.citation.issue17pt_PT
oaire.citation.startPage9311pt_PT
oaire.citation.titleCellulosept_PT
oaire.citation.volume29pt_PT
person.familyNameSantos Pereira
person.familyNameMoreira
person.givenNameAna Cláudia
person.givenNameFelismina
person.identifier1589429
person.identifier.ciencia-id8A10-2A44-A62C
person.identifier.ciencia-idC917-6A46-A270
person.identifier.orcid0000-0003-1727-8255
person.identifier.orcid0000-0003-4237-8952
person.identifier.scopus-author-id23486193000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication14e93b0b-1bed-4868-bb7a-0ce1d6e666f8
relation.isAuthorOfPublication9c13153d-a73a-49ff-b7f6-c9733bc6725e
relation.isAuthorOfPublication.latestForDiscovery9c13153d-a73a-49ff-b7f6-c9733bc6725e

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ART9_BioMark_Correia_2022.pdf
Size:
827.87 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – BioMark – Artigos