Publication
Ensuring food safety: electrochemical genosensors for the authentication of plant honey origin
| dc.contributor.author | Morais, Stephanie L. | |
| dc.contributor.author | Pereira, Eduarda | |
| dc.contributor.author | Castanheira, Michelle | |
| dc.contributor.author | Santos, Marlene | |
| dc.contributor.author | Domingues, Valentina | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.contributor.author | Barroso, M. Fátima | |
| dc.date.accessioned | 2025-01-17T12:32:37Z | |
| dc.date.available | 2025-01-17T12:32:37Z | |
| dc.date.issued | 2024-11 | |
| dc.description.abstract | Honey is a high-quality and natural ingredient often consumed because of its unique sweet taste and multiple therapeutic and nutritional benefits. These properties are normally intrinsically connected to the regional flora from which the plant pollen is harvested. Hence, the botanical and geographical origins of honeys play a substantial role in the end product's composition. With the recent interest in natural food products many businesses, including the honey industry, have observed a significant expansion in production and market value. However, honey is susceptible to adulteration and, as more and more adulterated honeys are being found on the global market, it is difficult to monitor the safety and quality of all honey products, making honey fraud a serious problem for both consumers and the food industry. Some of the most prevalent fraudulent acts include mislabeling the botanical and geographic origin of honeys and mixing pure honey with inferior honeys, processed sugars, syrups, and other substances. Thus, there is a need to develop an analytical tool that can quickly, cheaply, and easily guarantee the quality and safety of honey. In this study, an electrochemical genosensor, based on a sandwich format DNA hybridization reaction between two complementary probes, for the detection and quantification of two pollen producing plant species: Erica arborea and Castanea sativa were designed and optimized. Analyzing public databases, two synthetic DNA-target sequences capable of unequivocally detecting the pollen from E. arborea and C. sativa were selected and designed. Their complementary oligonucleotide probes were also designed and cut into two distinct sequences: the DNA-capture and DNA-signaling probes. In order to recognize the two plant species in real honey and pollen DNA samples and optimize the hybridization procedure, a mixed selfassembled monolayer of each plant species’ DNA-capture probe and mercaptohexanol was used. Then, the electrochemical signal was enzymatically amplified using chronoamperometric measurements. A concentration range of 0.03 to 2.00 nM for E. arborea and 0.03 to 1.00 nM for C. sativa were obtained. The developed sensors were successfully applied for the detection and quantification of the two plant species in real plant samples and, thus, indicate the botanic origin of honeys. Therefore, the developed electrochemical genosensors are a viable and affordable analytical tool to authenticate the botanical origin of honeys, ensuring honey quality and safety for consumers as well as the industries. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Morais, S. L., Pereira, E., Castanheira, M., Santos, M., Domingues, V., Delerue-Matos, C., & Barroso, F. (2024). Ensuring food safety: Electrochemical genosensors for the authentication of plant honey origin. XXVIII Encontro Galego‐Portugués de Química, 192. https://www.colquiga.org/_files/ugd/398543_a760c50141f64a86b13599425a6db0bd.pdf | pt_PT |
| dc.identifier.isbn | 978-84-09-66439-9 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/27113 | |
| dc.language.iso | eng | pt_PT |
| dc.publisher | Colegio Oficial de Químicos de Galicia | pt_PT |
| dc.relation | This work received financial support from national funds (FCT/MCTES, Fundação para a Ciência e Tecnologia and Ministério da Ciência, Tecnologia e Ensino Superior) through project MTS/SAS/0077/2020—“Honey+—New reasons to care honey from the Natural Park of Montesinho: A bioindicator of environmental quality & its therapeutic potential” and through the projects UIDB/50006/2020 and UIDP/50006/2020. MFB thanks Fundação para a Ciência e a Tecnologia (FCT) for the FCT Investigator (2020.03107.CEECIND). Stephanie Morais (2023.028929.BD) and Michelle Castanheira (2023.05159.BDANA) are grateful to FCT and the European Union (EU) for their grants financed by POPH-QREN-Tipologia 4.1-Formação Avançada, funded by Fundo Social Europeu (FSE) and Ministério da Ciência, Tecnologia e Ensino Superior (MCTES). | pt_PT |
| dc.relation.publisherversion | https://www.colquiga.org/_files/ugd/398543_a760c50141f64a86b13599425a6db0bd.pdf | pt_PT |
| dc.subject | Electrochemical genosensors | pt_PT |
| dc.subject | Plant honey origin | pt_PT |
| dc.title | Ensuring food safety: electrochemical genosensors for the authentication of plant honey origin | pt_PT |
| dc.type | conference object | |
| dspace.entity.type | Publication | |
| oaire.citation.conferencePlace | Vigo-Galiza-Espanha | pt_PT |
| oaire.citation.startPage | 192 | pt_PT |
| oaire.citation.title | XXVIII Encontro Galego‐Portugués de Química | pt_PT |
| person.familyName | Santos | |
| person.givenName | Marlene | |
| person.identifier | 1508370 | |
| person.identifier.ciencia-id | 8311-B967-31C4 | |
| person.identifier.orcid | 0000-0001-5020-5942 | |
| person.identifier.scopus-author-id | 57110502000 | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | conferenceObject | pt_PT |
| relation.isAuthorOfPublication | 8ce9ee39-a4c6-46ae-99e2-49397b550f1b | |
| relation.isAuthorOfPublication.latestForDiscovery | 8ce9ee39-a4c6-46ae-99e2-49397b550f1b |