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Advisor(s)
Abstract(s)
Quick, easy, cheap, effective, rugged, and safe extraction is a modern sample preparation
method that involves a number of steps with a low susceptibility to error. The efficiency
of a laboratory can be improved not only through labor reduction and consumable savings
but also through the use of high-throughput methods and the reduction of wastes. In
commercially available kits, different salts and buffers are mixed in anhydrous packages.
The composition of these kits are optimized and fixed for particular applications by the
suppliers. In this work three model compounds (ibuprofen and two of its main metabolites:
hydroxyibuprofen and carboxyibuprofen) were chosen and the amount of each salt (magnesium
sulfate, sodium chloride, sodium citrate, and disodium citrate sesquihydrate) in
the quick, easy, cheap, effective, rugged, and safe content was optimized for three different
soil samples (soils A, B, and C) with different organic carbon contents, using a 24 factorial
design. The optimized extraction procedure was applied to 12 soil samples; ranging from
river sediments to agricultural soils. Based on the analysis 100 samples, a price reduction
of 5.1- (soil B), 5.7- (Soil C), and 6.1-fold (soil A) was achieved without compromising the
performance of the method when compared to commercial kits.
Description
Keywords
Ibuprofen Metabolites QuEChERS Response surface methodology Soil
Citation
Publisher
Wiley-VCH Verlag