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Publication
Improving honey authentication: Electrochemical genosensors for the detection of Erica arborea in commercial honey
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Advisor(s)
Abstract(s)
Monofloral honeys, like heather honey, are a highly sought-after ingredient. However, food safety concerns have emerged due to the number of adulterated honeys found in the global market [1,2]. Thus, this work aimed to develop an electrochemical genosensor capable of detecting the residual DNA of Erica arborea (heather flower) in commercial honey samples. Analyzing public databases, a 98-mer E. arborea DNA-target probe and its complementary sequence were selected and designed. The developed genosensor was constructed on screen-printed gold electrodes. The electrochemical signal resulted from the sandwich-format hybridization reaction between the DNA-target and its complementary probe, previously cut into a 28-mer DNA-capture probe and a 70-mer DNA-signaling probe labeled with a fluorescein. Honeys samples were acquired from the local markets and their DNA extracted and amplified using DNA kits. Employing chronoamperometry, a linear correlation was obtained in a 0.03 to 2.00 nM concentration range. The DNA from the purchased honey samples were successfully extracted and applied to the genosensor. The developed electrochemical genosensor was able to detect the presence of E. arborea in the analyzed honey samples. This device is a promising analytical tool to combat honey fraud, facilitate honey authenticity and promote food safety.
Description
Keywords
Eletrochemical genosesnsors Erica arborea Food safety Honey authentication
Pedagogical Context
Citation
Morais, S., Castanheira, M., Santos, M., Domingues, V., Delerue-Matos, C., & Barroso, M. F. (2025). Improving honey authentication: Electrochemical genosensors for the detection of Erica arborea in commercial honey. Livro de Resumos do 18º Encontro de Investigação Jovem da U.Porto, 183–184. https://www.up.pt/ijup/wp-content/uploads/sites/892/2025/06/Livro-de-Resumos_IJUP-2025.pdf
Publisher
Universidade do Porto
CC License
Without CC licence