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- Sensitive ratiometric imprinted hydrogel for the detection of matrix metalloproteinase 7 (MMP7) biomarkerPublication . Piloto, Ana Margarida L.; Ribeiro, David S.M.; Santos, João L.M.; Sales, GoretiA dual-emissive fluorescent probe has been developed for the sensitive and selective detection of matrix metalloproteinase 7 (MMP7), a protein biomarker associated with pancreatic cancer1,2. The resultant materials were designated as imprinted ratiometric hydrogels (imprinted rHGs). The fluorescence quenching of the imprinted rHGs occurred with increasing concentrations of MMP7, showing linearity in the range [1.49 x 10-11 – 1.92 x 10-9] g/mL in 1000-fold diluted human serum in PBS 10mM pH 5.8. Overall, the imprinted rHGs developed in this work presented increased selectivity for the MMP7 protein over raw emitting QDs nanoparticles and higher sensitivity comparatively to the non-imprinted rHGs.
- Cellulose‑based hydrogel on quantum dots with molecularly imprinted polymers for the detection of CA19‑9 protein cancer biomarkerPublication . Piloto, Ana Margarida L.; Ribeiro, David S.M.; Rodrigues, S. Sofia M.; Santos, João L.M.; Sampaio, Paula; Sales, Maria Goreti FerreiraMolecularly imprinted polymers (MIPs), were successfully assembled around quantum dots (QDs), for the detection of the carbohydrate antigen CA19-9, a biomarker associated with pancreatic cancer (PC). The imprinted materials MIP@QDs were incorporated within cellulose hydrogels and designated as imprinted hydrogels (iHGs). The concept is to use MIPs which function as the biorecognition elements, conjugated to QDs as the sensing system. The fluorescence quenching of the iHGs occurred with increasing concentrations of CA19-9, showing linearity in the range 2.76 × 10−2 – 5.23 × 102 U/ml, in a 1000-fold diluted human serum. The iHGs show a linear response below the cutoff values for pancreatic cancer diagnosis (< 23 U/ml), a limit of detection of 1.58 × 10−3 U/ml and an imprinting factor (IF) of 1.76. Moreover, they exhibit stability and selectivity towards CA19-9, when compared with the non-imprinted controls, here designated as non-imprinted hydrogels (non-iHGs). In conclusion, this work demonstrates that the conjugation of MIPs to QDs increases the sensitivity and specificity of the developed sensing system for optical detection methods within clinical significance ranges. This fact shows potential for the iHGs to be applied as a sensitive, low-cost format for point-of-care tests (PoCTs).