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- Plastic antibody for the diagnosis of acute myocardial infarctionPublication . Ricardo, Jacinta; Martins, Gabriela; Moreira, Felismina T.C.(Introduction) Novel application Plastic Antibody that responds to a cardiac biomarker, myoglobin (Myo). Imprint stage with electropolymerization of ortho-phenylenediamine (OPD) in the presence of Myo. Template removal from polymeric matrix digested by trypsin. The films acted as biomimetic artificial antibodies and were fabricated on a screen-printed platinium (Pt) electrode (SPE) modified with electroactive Prussian blue nanocubes (PBNCs) to take a step towards disposable sensors for point-of-care applications. The devices showed linear responses to Myo in SWV assays up to 0.01 and 10000 ng/mL.
- Plastic antibody for the diagnosis of acute myocardial infarctionPublication . Ricardo, Jacinta; Martins, Gabriela V.; Moreira, Felismina T.C.(Introduction) Myoglobin (Myo) is the first cardiac biomarker to be released into the bloodstream after the onset of symptoms of Acute Myocardial Infarction (AMI), allowing earlier detection of this disease. Novel application based on Plastic Antibody that responds to a cardiac biomarker, Myo. Imprint stage with electropolymerization of ortho-phenylenediamine (OPD) in the presence of Myo. Template removal from polymeric matrix digested by trypsin. The biomimetic film was fabricated on platinum screen-printed electrodes (Pt-SPE) modified with electroactive Prussian blue nanocubes (PBNCs).
- Enhanced detection with prussian blue-based nanocubes: a novel electrochemical biosensor for bovine serum albumin analysis: microfluidic integrationPublication . Santos, Andréa dos; Oliveira, Daniela; Martins, Gabriela; Moreira, Felismina T.C.(Introduction) Early diagnosis of biomarkers is crucial for optimizing treatments and increasing survival rates. Direct and cost-effective assays are important for point-of-care (PoC) testing. Bovine serum albumin (BSA) monitoring indicates liver and kidney function and aids in the assessment of diseases such as cirrhosis and chronic kidney disease. An innovative electrochemical biosensor was developed in which a platinum electrode was decorated with manganese-based Prussian blue nanocubes (PB-NC). A molecularly imprinted polymer (MIP) was created on the electrode surface by electropolymerization of phenol in the presence of BSA using cyclic voltammetry (CV). The template was removed with proteinase K and a mixture of methanol and acetic acid. Square wave voltammetry (SWV) characterized the performance of the sensor, which exhibited high sensitivity and allowed detection of BSA at nanomolar levels.