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- Fluorescence-based biosensor for SARS-CoV-2 detection and quantificationPublication . Duarte, Abel José Assunção; Gonçalves, Helena Maria Rodrigues; Lopes, Paula Filomena MartinsThe present disclosure relates to a method for detection of biomolecules comprising the steps of collecting a biological sample comprising an analyte at a concentration of at least 0.01 pM; exposing the collected sample to a temperature ranging from 70-100 °C; incubating the sample with Cdots previously incubated with the probe, therefore obtaining a solution; carefully homogenising the resulting solution; subjecting the solution to an excitation source for acquisition of fluorescence; determining the fluorescence shift between a negative control and the sample; wherein the Cdots are in a suspension state and the proportion of Cdots for analyte in the sample are in a ratio of at least 1:0.5; and wherein a shift of fluorescence from the negative control to the sample indicates the presence of the biomolecule. The present disclosure also relates to an apparatus for carrying out the process according to the present invention comprising a sensing unit (A), connected by an optical fiber (B) to a sample holder (C), and a LED (D) and a kit for detection of biomolecules, in particular for diagnosis of SARS-CoV-2.
- Midfoot-equipped sole for arch support and plantar pressure redistributionPublication . Marques, Maria Arcelina; Ramos, Nuno Viriato Marques; Vaz, Mário Augusto Pires de; Flores, Andreia Manuela Crespo; Martins, Cátia Sofia Neves; Silva, Bruno Ricardo Barreto Guedes daThe invention stated herein concerns a solution for shoe soles which are adaptable to plantar arch morphology. The solution is essentially characterized by combining. Into a single sole. Arch support (4) internally as an integral component of the sole (2) and an insert (1) having a smaller young's modulus than that of the remaining sole (2) externally as an integral component of the sole and confined to the midfoot region (3). This combination of characteristics, which increases the contact area between the foot and the shoe, at the insert of the sole, provides for a more effective damping of plantar forces and leads to a more efficient solution regarding the redistribution and decrease of the maximum forces that act in the midfoot region.
- Método de criação de janelas óticas de diagnóstico e tratamento em materiais biológicos na zona do ultravioletaPublication . Oliveira, Luís; Carneiro, Isa; Carvalho, Sónia; Henrique, Rui; Tuchin, ValeryPatente nacional submetida a março de 2019 e publicada a 14 de setembro de 2020. PT115371 (A)