Browsing by Author "Crespo, Paulo"
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- Assessing radiobiological effects of diagnostic level doses of ionizing radiation on zebrafish using comet assayPublication . Lemos, Joana; Costa, Pedro; Cunha, Lídia; Carvalho, António Paulo; Neuparth, Teresa; Genésio, Paula; Ponte, Fernanda; Costa, Paulo; Crespo, Paulo; Metello, Luís F.This paper relates with the application of zebrafish – Danio rerio – to the study of radiobiological effects of medical diagnostic level – low doses – of ionizing radiation. In recent years, the use of zebrafish has grown considerably, pointing more and more as a very interesting model in biomedical research, essentially because of the level of homology shared with the human genome, complemented by an easy and reasonably affordable practical side.
- Assessing Radiobiological Effects of Low Doses of Ionizing Radiation on Zebrafish by Bidimensional Gel ElectrophoresisPublication . Lemos, Joana; Costa, Pedro; Cunha, Lídia; Campos, Alexandre; Carvalho, António Paulo; Vasconcelos, Vítor; Genésio, Paula; Ponte, Fernanda; Costa, Paulo; Crespo, Paulo; Metello, Luís F.Aim: This paper relates with a specific task from a larger research project oriented to the development and application of the Zebrafish – Danio rerio – to the study of radiobiological effects of medical diagnostic level – low doses – of ionizing radiation.
- Developing advanced biological models to study effects of low doses of ionizing radiation: our experience.Publication . Lemos, Joana; Costa, Pedro; Cunha, Lídia; Carvalho, António P.; Vasconcelos, Vítor; Silva, Regina; Fernandes, Sílvia; Santos, João; Cunha, Micaela; Crespo, Paulo; Metello, Luís F.; Alves, F.The biological effects of high doses of ionizing radiation are reasonably well studied and documented. Nevertheless, there is a clear lack of evidence concerning biological effects of Low Doses, namely at medical imaging levels, as those characterizing Nuclear Medicine and Radiology typical environments. Recognizing limitations and translational difficulties of the actual monolayer cellular models, our group has produced efforts to develop advanced biological models, namely using three-dimensional cell cultures and a less explored animal model (Zebrafish - which allow access to inter-generational data, while characterized by a great genetic homology). This work aims to exhibit preliminary results already obtained
- Evaluation of Radiobiological Effects in Three Distinct Biological ModelsPublication . Lemos, Joana; Costa, Pedro; Cunha, Lídia; Carvalho, António Paulo; Vasconcelos, Vítor; Genésio, Paula; Ponte, Fernanda; Costa, Paulo; Crespo, Paulo; Metello, Luís F.The present work aims to share the process of development of advanced biological models to study radiobiological effects. Recognizing several known limitations and difficulties of the current monolayer cellular models, as well as the increasing difficulties to use advanced biological models, our group has been developing advanced biological alternative models, namely three- dimensional cell cultures and a less explored animal model (the Zebrafish - Danio rerio - which allows the access to inter-generational data, while characterized by a great genetic homology towards the humans). These three models (monolayer cellular model, three-dimensional cell cultures and zebrafish) were externally irradiated with 100mGy, 500mGy or 1Gy. The consequences of that irradiation were studied using cellular and molecular tests.
- Low dose irradiation of three-dimensional cell cultures has an acute effect on proliferation ratePublication . Costa, Pedro; Lemos, Joana; Cunha, Lídia; Genésio, Paula; Ponte, Fernanda; Costa, Paulo S.; Crespo, Paulo; Metello, Luís F.Low doses of ionizing radiation are not yet consistently studied and documented. In such context, more advanced and complex biological models should be applied to obtain relevant data. Recognizing several known limitations and difficulties of the current monolayer cellular models, as well as the increasing difficulties to use advanced biological models, our group has been developing advanced biological alternative models, namely three-dimensional cell cultures. These cellular models (monolayer cellular culture and three-dimensional cell cultures - cultured with encapsulation in 1% alginate and 1,5% agarose coating) were externally irradiated with 100mGy and 1Gy. The consequences of that irradiation were studied in terms of proliferation rate (using MTT assay) and DNA damage (using comet assay), 24h and 72h post-irradiation.
- Modern Radiobiology: Study of Ionizing Radiation Biological Effects using ThreeDimensional Cell CulturesPublication . Costa, Pedro; Lemos, Joana; Cunha, Lídia; Carvalho, António P.; Vasconcelos, Vítor; Genésio, Paula; Ponte, Fernanda; Costa, Paulo S.; Crespo, Paulo; Metello, Luís F.Biological effects of high doses of ionizing radiation are reasonably well studied and documented. Nevertheless, there is a clear lack of information and/or evidence concerning biological effects of Low Doses, namely at medical imaging levels, as those characterizing Nuclear Medicine and Radiology typical environments. Our group has being involved in the in vitro study of these low dose radiobiological effects. Preliminary experimental results with 100mGy external gamma irradiation of HepG2 monolayer cells showed a slight increase in the proliferation rate 24h, 48h and 72h post irradiation. These preliminary results also pointed into the presence of some Bystander effects 72h post irradiation, constituting the starting point and motivation for the need of a more accurate analysis , here presented.
- The acute effect on three-dimensional cellular proliferation rate of low dose irradiation exposuresPublication . Costa, Pedro; Lemos, Joana; Cunha, Lídia; Neuparth, Teresa; Genésio, Paula; Ponte, Fernanda; Costa, Paulo; Crespo, Paulo; Metello, LuísLow doses of ionizing radiation are not yet consistently studied and documented. In such context, more advanced and complex biological models should be applied to obtain relevant data. Known limitations of monolayer cellular models and ever increasing difficulties to use some biological models, lead to develop advanced biological models, namely three-dimensional cell cultures.