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Low doses of ionizing radiation are not yet consistently studied and documented. In such context, more advanced and complex biological models should be applied to obtain relevant data.
Recognizing several known limitations and difficulties of the current monolayer cellular models, as well as the increasing difficulties to use advanced biological models, our group has been developing advanced biological alternative models, namely three-dimensional cell cultures. These cellular models (monolayer cellular culture and three-dimensional cell cultures - cultured with encapsulation in 1% alginate and 1,5% agarose coating) were externally irradiated with 100mGy and 1Gy. The consequences of that irradiation were studied in terms of proliferation rate (using MTT assay) and DNA damage (using comet assay), 24h and 72h post-irradiation.