Browsing by Author "Carneiro, Mariana"
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- Assessing radiobiological effects of low doses of ionizing radiation on zebrafish muscle by two-dimensional gel electrophoresisPublication . Lemos, Joana; Campos, Alexandre; Carneiro, Mariana; Ribeiro, Tiago; Ponte, Fernanda; Costa, Paulo; Cunha, Lídia; Carvalho, António Paulo; Metello, Luís F.Introduction: This work relates with the application of zebrafish - Danio rerio - to the study of radiobiological effects of low doses of ionizing radiation. In recent years, the use of zebrafish has grown considerably, pointing more and more as a very interesting model in biomedical research, essentially because of the level of homology shared with the human genome, complemented by an easy and reasonably affordable practical side. The two-dimensional gel electrophoresis (2DGE) is a method used for the analysis of complex protein mixtures from biological samples. This method allows the detection of differences in protein expression under different conditions, so allowing creating relations between those differences and the irradiations that were in their basis. The present work aimed at evaluating proteome changes in the zebrafish muscle caused by low doses of X-rays exposure.
- Colorimetric Paper-Based Sensors against Cancer BiomarkersPublication . Carneiro, Mariana; Rodrigues, R. V.; Moreira, Felismina; Ferreira Sales, Maria GoretiCancer is a major cause of mortality and morbidity worldwide. Detection and quantification of cancer biomarkers plays a critical role in cancer early diagnosis, screening, and treatment. Clinicians, particularly in developing countries, deal with high costs and limited resources for diagnostic systems. Using low-cost substrates to develop sensor devices could be very helpful. The interest in paper-based sensors with colorimetric detection increased exponentially in the last decade as they meet the criteria for point-of-care (PoC) devices. Cellulose and different nanomaterials have been used as substrate and colorimetric probes, respectively, for these types of devices in their different designs as spot tests, lateral-flow assays, dipsticks, and microfluidic paper-based devices (μPADs), offering low-cost and disposable devices. However, the main challenge with these devices is their low sensitivity and lack of efficiency in performing quantitative measurements. This review includes an overview of the use of paper for the development of sensing devices focusing on colorimetric detection and their application to cancer biomarkers. We highlight recent works reporting the use of paper in the development of colorimetric sensors for cancer biomarkers, such as proteins, nucleic acids, and others. Finally, we discuss the main advantages of these types of devices and highlight their major pitfalls.
- Microbiological evaluation of vegetable salads in school canteensPublication . Carneiro, Mariana; Amorim, Manuela; Ferreira, Stephanie; Mota, Sandra; Moreira, Anabela; Augusto, AntónioCurrently bacteria are the main responsible for infections and food poisoning, being a huge public health problem worldwide. Vegetables are potential vehicles of these microorganisms and their consumption, especially raw, increases contamination risk. It has been reported an increase in diseases associated with microbiological contamination by vegetables consumption. Vegetable cultivation and growth process makes them vulnerable to contamination sources that may act at any stage from planting to consumption. Salmonella, Escherichiacoli , Staphylococcus and Listeria are the microorganisms most commonly isolated from vegetables responsible for food borne diseases.