ESS - TBIO - Posters apresentados em eventos científicos
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Browsing ESS - TBIO - Posters apresentados em eventos científicos by Author "Baylina, Pilar"
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- Antimicrobial activity of food-isolated fungi extractsPublication . Ferreira, Diogo; Areal-Hermida, Lara; Baylina, Pilar; Fernandes, Rúben; Sieiro, CarmenOne major source for drug discovery are microbial metabolites. Fungi, renowned for their ability to produce an array of broad and diverse secondary metabolites, due to their extensive dispersion and diversity, offer a rich resource for drug discovery. Antibiotic resistance is a major concern. Rapid increase of resistant bacteria worldwide, dampens antibiotic efficiency, burdens healthcare services and increase morbidity and mortality. Antibiotic misuse and lack of new drug development are the main responsible for this health crisis. So, the creation of fungal libraries to find and study new compounds is essential to tackle the rising of antimicrobial resistance and continue with industrial efforts of drug discovery and production. Isolation from chestnuts, chestnut flour and sunflower seeds allowed us to obtain a collection of 165 fungal isolates. Bioactivity of fungal extracts were screened against different antibiotic resistant bacteria. Bacteria grown overnight, adjusted to 1.5 x 108 CFU/mL was exposed to fungal extracts, at a concentration of 100 μg/mL for 24 hours and inhibition rates were calculated. Several extracts showed activity against antimicrobial resistant bacteria and further studies should be made in order to find if new molecules could be responsible for our fungi antimicrobial activity.
- Avaliação do potencial antimicrobiano de fungos filamentosos: Um estudo promissor na abordagem do pé diabético e da resistência bacterianaPublication . Ferreira, D.; BAYLINA MACHADO, PILAR; Sá, S.; Areal Hermida, L.; Rocha, A. C.; Baylina, Pilar; Fernandes, R.; Sieiro, C.Prevê-se que até 2045, cerca de 700 milhões de pessoas possam ser afetadas pela Diabetes Mellitus (DM). Uma complicação frequente em indivíduos com DM é o pé diabético, que se manifesta por feridas nos pés causadas por danos nos nervos e vasos sanguíneos, resultando frequentemente na amputação dos membros inferiores. Esta situação é agravada por infeções bacterianas, causadas por estirpes resistentes, como Staphylococcus aureus resistente à meticilina (MRSA), Pseudomonas aeruginosa e Klebsiella spp. produtoras de beta-lactamases de espectro estendido (ESBL), dificultando o tratamento destas lesões. A resistência antibiótica, impulsionada pelo uso excessivo e indiscriminado de antibióticos, destaca a necessidade urgente de novos fármacos e terapias mais eficazes. Neste contexto, os fungos apresentam-se como uma fonte promissora de novos agentes antimicrobianos, devido à vasta gama e diversidade de compostos bioativos que conseguem sintetizar.
- Avaliação do potenicial antimicrobiano do extrato de Gnomoniopsis sp. contra agentes infeciosos do pé diabéticoPublication . Rocha, A. C.; Areal Hermida, L.; Baylina, Pilar; Fernandes, R.; Sieiro, C.; BAYLINA MACHADO, PILAREstima-se que até ao ano de 2045, aproximadamente 700 milhões de pessoas sofram de Diabetes mellitus (DM). O pé diabético é uma complicação comum em pacientes com DM e caracteriza-se por lesões nos pés devido a danos nos nervos e vasos sanguíneos, levando muitas vezes à amputação dos membros inferiores. A infeção causada por bactérias resistentes, como Staphyloccus aureus resistente a meticilina (MRSA), Pseudomonas aeruginosa e espécies de Klebsiella beta-lactamases de espetro estendido (ESBL), acentuam a gravidade destas lesões, tornando o seu tratamento mais complexo. A resistência a antibióticos resulta do uso exagerado e indiscriminado de antibióticos e o desenvolvimento de medicamentos inovadores e de terapias mais eficazes é urgente. Assim, os fungos, nomeadamente fungos filamentosos, surgem como um potencial reservatório para novos compostos antimicrobianos, devido à grande quantidade e diversidade de compostos bioativos produzidos por estes organismos.
- Creation of a fungal library and screening of antimicrobial and anticancer activityPublication . Ferreira, Diogo; Hermida, Lara Areal; Rocha, Ana Catarina; Baylina, Pilar; Sieiro, Carmen; Fernandes, Rúben; BAYLINA MACHADO, PILARAccording to the World Health Organization, cancer and infectious diseases are two of the most problematic diseases nowadays. Cancer kills 10 million people every year and the emergence of resistance to antitumoral drugs is an important medical challenge. At the same time, antimicrobial resistance (AMR) is also a serious threat to human and environmental health. Besides mortality, AMR burdens healthcare services and dampens medical procedures such as surgeries, cancer treatments and other invasive procedures. The development of new drug therapies to fight drug resistance is essential to contest the rising of resistant bacteria and reduction of the effectiveness of antitumoral drugs. Microorganisms have been a major source for natural compounds throughout the years. Fungi, renowned for their ability to produce an array of broad and diverse secondary metabolites, offer a rich resource for drug discovery. We built a collection of fungal species, isolated from chestnuts, sunflower seeds, and chestnut flour, and explored their extracts for potential antimicrobial and anticancer activity. Fungi cultures for secondary metabolite biosynthesis were done in submerged fermentation in Malt Extract broth for 15 days at 26 °C. Liquid-liquid extraction techniques, with ethyl acetate as a solvent, were applied to obtain crude secondary metabolite extracts. Clinical resistant bacteria, yeasts, and prostate cell lines (human prostate epithelial cells – HpepiC; human caucasian prostate adenocarcinoma cells - PC3) were exposed to fungal extracts at a single concentration of 100 µg/mL. Our results so far show several extracts with antimicrobial and/or anticancer activity without decreasing cell viability of non-tumoral cells, showing their potential as therapeutic drugs without possible secondary effects. Although, more studies should be done, and pending fungal identification will allow us to select which extracts will be further investigated to find if the displayed bioactivity could be happening due to unknown natural compounds
- Potential anticancer activity from food-isolated fungi extractsPublication . Ferreira, Diogo; Rocha, Ana Catarina; Baylina, Pilar; Sieiro, Carmen; Fernandes, RúbenFungal species have demonstrated great potential to produce a wide range of metabolites, including enzymes, antibiotics, and other bioactive compounds with therapeutic interest. Prostate cancer (PCa) is one of the most frequent cancers in men. This type of tumors have high levels of heterogeneity, leading to therapeutic failures and increasing resistance against chemotherapeutic drugs. Hence, is essential to research new therapeutic agents against PCa. Exploring the rich reservoir of fungal diversity, this study aims to uncover bioactive compounds that may serve as valuable candidates for developing novel therapeutics against prostate cancer. Isolation from chestnuts, chestnut flour and sunflower seeds led to the creation of a fungal collection of 165 isolates. Fungi isolates grew in flask cultures for 15 days, and culture broths were extracted with ethyl acetate. Human prostate epithelial cells (HPepiC) and the human prostate cancer cell line (PC3) were exposed to the fungal extracts at a concentration 100 μg/mL, and cell viability was evaluated by MTT assay. Results show that several fungal extracts significantly reduce the viability of tumor cells, with some showing little to no effect on healthy human cells, however, species identification is essential to carry on our studies.