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Os laboratórios CIIMAR@E2S resultam da integração do P.PORTO como membro associado do Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR). Esta integração tem vindo a ser progressivamente consolidada na E2S através do envolvimento de longa data de investigadores integrados no CIIMAR. A criação dos laboratórios CIIMAR@E2S reforçou a colaboração entre as instituições e potenciou o aumento da capacidade de investigação nas áreas da biotecnologia azul, saúde, ambiente e sustentabilidade. A investigação desenvolvida centra-se principalmente no desenvolvimento de soluções funcionais, inovadoras, seguras e sustentáveis, com aplicações nos domínios da saúde e do ambiente. Na área da biotecnologia aplicada à saúde, a investigação incide na descoberta de compostos naturais com potencial de intervenção em doenças neurodegenerativas e no cancro, bem como no desenvolvimento de alternativas naturais para dermocosméticos e nutracêuticos. No domínio da sustentabilidade ambiental, a investigação está centrada no desenvolvimento de pigmentos derivados de microrganismos para aplicações industriais e na valorização de resíduos têxteis e agroalimentares.
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Percorrer CIIMAR@E2S - Aceda ao link para visualizar publicações por autor "Almeida, Agostinho"
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- Analysis of the Use of Cylindrospermopsin and/or Microcystin-Contaminated Water in the Growth, Mineral Content, and Contamination of Spinacia oleracea and Lactuca sativaPublication . Llana-Ruiz-Cabello, Maria; Jos, Angeles; Cameán, Ana; Oliveira, Flavio; Barreiro, Aldo; Machado, Joana; Azevedo, Joana; Pinto, Edgar; Almeida, Agostinho; Campos, Alexandre; Vasconcelos, Vitor; Freitas, MarisaCyanobacteria and cyanotoxins constitute a serious environmental and human health problem. Moreover, concerns are raised with the use of contaminated water in agriculture and vegetable production as this can lead to food contamination and human exposure to toxins as well as impairment in crop development and productivity. The objective of this work was to assess the susceptibility of two green vegetables, spinach and lettuce, to the cyanotoxins microcystin (MC) and cylindrospermopsin (CYN), individually and in mixture. The study consisted of growing both vegetables in hydroponics, under controlled conditions, for 21 days in nutrient medium doped with MC or CYN at 10 μg/L and 50 μg/L, or CYN/MC mixture at 5 + 5 μg/L and 25 + 25 μg/L. Extracts from M. aeruginosa and C. ovalisporum were used as sources of toxins. The study revealed growth inhibition of the aerial part (Leaves) in both species when treated with 50µg/L of MC, CYN and CYN/MC mixture. MC showed to be more harmful to plant growth than CYN. Moreover spinach leaves growth was inhibited by both 5 + 5 and 25 + 25 µg/L CYN/MC mixtures, whereas lettuce leaves growth was inhibited only by 25 + 25 µg/L CYN/MC mixture. Overall, growth data evidence increased sensitivity of spinach to cyanotoxins in comparison to lettuce. On the other hand, plants exposed to CYN/MC mixture showed differential accumulation of CYN and MC. In addition, CYN, but not MC, was translocated from the roots to the leaves. CYN and MC affected the levels of minerals particularly in plant roots. The elements most affected were Ca, K and Mg. However, in leaves K was the mineral that was affected by exposure to cyanotoxins.
- Effects of Chrysosporum (Aphanizomenon) ovalisporum extracts containing cylindrospermopsin on growth, photosynthetic capacity, and mineral content of carrots (Daucus carota)Publication . Guzmán-Guillén, Remedios; Campos, Alexandre; Machado, Joana; Freitas, Marisa; Azevedo, Joana; Pinto, Edgar; Almeida, Agostinho; Cameán, Ana M.; Vasconcelos, VitorNatural toxins produced by freshwater cyanobacteria, such as cylindrospermopsin, have been regarded as an emergent environmental threat. Despite the risks for food safety, the impact of these water contaminants in agriculture is not yet fully understood. Carrots (Daucus carota) are root vegetables, extensively consumed worldwide with great importance for human nourishment and economy. It is, therefore, important to evaluate the possible effects of using water contaminated with cyanotoxins on carrot cultivation. The aim of this work was to investigate cylindrospermopsin effects on D. carota grown in soil and irrigated for 30 days, with a Chrysosporum ovalisporum extract containing environmentally relevant concentrations of cylindrospermopsin (10 and 50 μg/L). The parameters evaluated were plant growth, photosynthetic capacity, and nutritional value (mineral content) in roots of carrots, as these are the edible parts of this plant crop. The results show that, exposure to cylindrospermopsin did not have a clear negative effect on growth or photosynthesis of D. carota, even leading to an increase of both parameters. However, alterations in mineral contents were detected after exposure to crude extracts of C. ovalisporum containing cylindrospermopsin. A general decline was observed for most minerals (Ca, Mg, Na, Fe, Mn, Zn, Mo, and P), although an increase was shown in the case of K and Cu, pointing to a possible interference of the cyanobacterial extract in mineral uptake. This study is the first to evaluate the effects of C. ovalisporum extracts on a root vegetable, however, more research is necessary to understand the effects of this toxin in environmentally relevant scenarios.
- Sustainable agriculture with cyanobacterial biomass: Soil experiments on spinach and radish plantsPublication . Massa, Anabella; Martins, Diogo; Azevedo, Joana; Prieto, Ana I.; Camean, Ana M.; Diez-Quijada, Leticia; Jos, Angeles; Reimão, Mariana; Azevedo, Rui; Pinto, Edgar; Almeida, Agostinho; Vasconcelos, Vitor; Campos, Alexandre; Freitas, Marisa; REIMÃO BORGES LOPES DA SILVA, MARIANATo support sustainable food production and improve crop yields, it is essential to explore bio-based plant growth- promoting products. Cyanobacterial biomass has shown potential to enhance soil quality and agricultural productivity. However, some cyanobacteria produce cyanotoxins, such as microcystins, cylindrospermopsin and anatoxin-a, which can adversely affect plant development depending on their concentration. This study evaluated the potential of cyanobacterial biomass as a soil amendment and plant growth stimulant by assessing growth, nutrient content and toxin accumulation in spinach and radish plants. Plants were cultivated in soil treated with no amendment, commercial fertilizer, or cyanobacterial biomass from Microcystis aeruginosa (microcystins-producer), Anabaena sp. (anatoxin-a-producer), Raphidiopsis raciborskii (non-cylindrospermopsin producer), and R. raciborskii (cylindrospermopsin-producer). While biomass additions supplied nutrients to the soil, spinach showed increased sensitivity to microcystins and anatoxin-a, with significantly reduced growth. Overall, mineral concentrations in plant tissues did not increase; most macro- and micronutrients declined, particularly in spinach shoots and radish roots (p ≥ 0.05). Vitamin C content also decreased in most treatments, except in plants treated with the non-cylindrospermopsin-producing R. raciborskii strain, where a significant increase was observed (p ≥ 0.05). Toxic biomass amendments led to the uptake of microcystins-LR and cylindrospermopsin in spinach, and cylindrospermopsin in radish, with cylindrospermopsin levels in spinach exceeding Word Health Organization's tolerable daily intake. These findings underscore the need for a previous detailed characterization of both nutrients and toxins in cyanobacterial biomass to ensure its safe and effective agricultural use, maximizing benefits while protecting food safety.
- Toxic and non-toxic cyanobacterial biomass as a resource for sustainable agriculture: A lettuce cultivation experimentPublication . Massa, Anabella; Santos, Érica; Martins, Diogo; Azevedo, Joana; Reimão, Mariana; Almeida, Agostinho; Azevedo, Rui; Pinto, Edgar; Vasconcelos, Vítor; Campos, Alexandre; Freitas, MarisaCyanobacteria represent a promising resource for sustainable agriculture, as they have demonstrated the ability to restore soil fertility even after death and decay. However, several cyanobacteria can also release secondary metabolites, such as cyanotoxins, which may compromise the quality of agricultural products and pose a potential risk to human health. Depending on the concentration of exposure, few studies reported deleterious effects on plant species when irrigated with cylindrospermopsin (CYN) contaminated water, impairing plant growth and leading to food product contamination, while other studies show promoting effects on plant yield. To evaluate the potential of cyanobacterial biomass (cyanotoxin-containing or not) as a sustainable resource for soil amendment, biostimulants or fertilizers for lettuce cultivation, a study was carried out that consisted of the culture of lettuce plants under controlled conditions, in soil: (1) with no extra nutrient addition (control) and supplemented with 0.6 g of freeze-dried Raphidiopsis raciborskii biomass of (2) a non-CYN-producing strain, (3) a CYN-producing strain, and (4) the same CYN-producing strain pasteurized. Results showed no significant differences in photosystem II efficiency with the amendment addition. On the contrary, shoot fresh weight significantly increased in lettuce plants grown with the cyanobacterial biomass addition, especially in condition (3). In addition, there were significant differences in mineral concentrations in lettuce leaves after the cyanobacterial biomass addition, such as K, Na, Ca, P, Mg, Mn, Zn, Cu, Mo, and Co. CYN accumulation was detected under conditions (3) and (4), with concentrations observed in descending order from roots > soil > shoot. Nevertheless, the CYN concentration in edible tissues did not exceed the WHO-proposed tolerable daily intake of 0.03 μg/kg/day. These findings suggest that incorporating cyanobacterial biomass as a soil amendment, biostimulant or fertilizer for lettuce cultivation, even with trace amounts of CYN (1–40 μg/g), may enhance plant yield without leading to cyanotoxin accumulation in edible tissues above the WHO-recommended tolerable daily intake.