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Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

2014Journal article Open access
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dc.contributor.authorFernandes, Rúben
dc.contributor.authorAmador, Paula
dc.contributor.authorOliveira, Carla
dc.contributor.authorPrudêncio, Cristina
dc.date.accessioned2019-05-20T14:11:37Z
dc.date.available2019-05-20T14:11:37Z
dc.date.issued2014
dc.description.abstractExtended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β -lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboring bla ESBL genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found for E. coli isolates and five clusters for K. pneumoniae strains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1155/2014/782897pt_PT
dc.identifier.issn2356-6140
dc.identifier.urihttp://hdl.handle.net/10400.22/13752
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.hindawi.com/journals/tswj/2014/782897/pt_PT
dc.subjectEnterobacteriaceaept_PT
dc.subjectIsoelectric Focusingpt_PT
dc.subjectPolymerase Chain Reactionpt_PT
dc.subjectPortugalpt_PT
dc.subjectbeta-Lactamasespt_PT
dc.titleMolecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage6pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleScientific World Journalpt_PT
oaire.citation.volume2014pt_PT
person.familyNameFernandes
person.familyNamePrudêncio
person.givenNameRúben
person.givenNameCristina
person.identifier635792
person.identifier1200571
person.identifier.ciencia-id0D1F-4090-E82A
person.identifier.ciencia-idC81E-F4EE-FADE
person.identifier.orcid0000-0001-8933-3984
person.identifier.orcid0000-0002-9920-936X
person.identifier.scopus-author-id57640135700
person.identifier.scopus-author-id6508057930
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4
relation.isAuthorOfPublication881a8ad5-ab13-4e49-89f4-08ca61cc81e3
relation.isAuthorOfPublication.latestForDiscovery9e6c397c-62d8-4a40-8ec7-ad05ae0ebcc4

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