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Chitosan-based silver nanoparticles: A study of the antibacterial, antileishmanial and cytotoxic effects

2016Journal article Open access
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dc.contributor.authorLima, Douglas dos Santos
dc.contributor.authorGullon, Beatriz
dc.contributor.authorCardelle-Cobas, Alejandra
dc.contributor.authorBrito, Lucas M
dc.contributor.authorRodrigues, Klinger AF
dc.contributor.authorQuelemes, Patrick V
dc.contributor.authorRamos-Jesus, Joilson
dc.contributor.authorArcanjo, Daniel DR
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorBatziou, Krystallenia
dc.contributor.authorQuaresma, Pedro
dc.contributor.authorEaton, Peter
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorCarvalho, Fernando Aecio A
dc.contributor.authorSilva, Durcilene Alves da
dc.contributor.authorPintado, Manuela
dc.contributor.authorLeite, Jose Roberto de Sá
dc.date.accessioned2019-06-06T14:01:10Z
dc.date.available2019-06-06T14:01:10Z
dc.date.issued2016
dc.description.abstractSilver nanoparticles have been studied as an alternative for treatment of microbial infections and leishmaniasis, without promoting induction of microbial or parasite resistance. In this study, chitosan-based silver nanoparticles were synthesized from silver nitrate (AgNO3), sodium borohydride as a reducing agent, and the biopolymer chitosan as a capping agent. The chitosan-based silver nanoparticles were characterized by ultraviolet–visible, Fourier transform infrared, dynamic light scattering, zeta potential, atomic force microscopy, and transmission electron microscope. The antibacterial assay was performed by determination of the minimum inhibitory concentration. The antileishmanial and the cytotoxic effects induced by AgNO3, chitosan, and chitosan-based silver nanoparticles were analyzed by resazurin and MTT colorimetric assays, respectively. AgNO3, chitosan, and chitosan-based silver nanoparticles induced a marked activity against all bacterial strains and promastigote forms of Leishmania amazonensis at minimum inhibitory concentrations ranging from 1.69 to 3.38 µg Ag/mL. Interestingly, the chitosan-based silver nanoparticles presented less cytotoxicity than the AgNO3 alone and were more active against L. amazonensis than solely chitosan. Furthermore, the cytotoxic concentrations (CC50) of both chitosan and chitosan-based silver nanoparticles against macrophages were significantly higher than the IC50 against promastigotes. Thus, the chitosan-based silver nanoparticles represent a promising alternative for the treatment of microbial infections and leishmaniasis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1177/0883911516681329pt_PT
dc.identifier.issn1530-8030
dc.identifier.urihttp://hdl.handle.net/10400.22/13883
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://journals.sagepub.com/doi/10.1177/0883911516681329pt_PT
dc.subjectAntibacterial activitypt_PT
dc.subjectAntileishmanial activitypt_PT
dc.subjectChitosanpt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectLeishmaniapt_PT
dc.subjectMTTpt_PT
dc.subjectResazurinpt_PT
dc.subjectSilver nanoparticlespt_PT
dc.titleChitosan-based silver nanoparticles: A study of the antibacterial, antileishmanial and cytotoxic effectspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage410pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage397pt_PT
oaire.citation.titleJournal of Bioactive and Compatible Polymerspt_PT
oaire.citation.volume32pt_PT
person.familyNamePlácido
person.familyNameDelerue-Matos
person.givenNameAlexandra
person.givenNameCristina
person.identifier1621053
person.identifier.ciencia-id1B1A-461B-E8DD
person.identifier.ciencia-id9A1A-43FB-5C27
person.identifier.orcid0000-0003-2706-7777
person.identifier.orcid0000-0002-3924-776X
person.identifier.ridL-3085-2014
person.identifier.ridD-4990-2013
person.identifier.scopus-author-id55232677900
person.identifier.scopus-author-id6603741848
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationaad1737a-79f4-4c05-a480-3c0197877757
relation.isAuthorOfPublication09f6a7bd-2f15-42b0-adc5-04bd22210519
relation.isAuthorOfPublication.latestForDiscovery09f6a7bd-2f15-42b0-adc5-04bd22210519

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