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PVA-based contact lenses incorporating Hyaluronic Acid and secretome for corneal repair
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Orientador(es)
Resumo(s)
Nowadays, people live much longer, and as a result, age-related degenerative diseases, such as corneal disorders, are more common, These conditions often lead to complications such as corneal opacity, neovascularization, and significant vision loss. Current treatment therapeutic approaches - such as topical eye drops and corneal transplantation - are limited by low regenerative capacity, poor bioavailability, scarcity of donor tissues, and the risk of immune rejection. This study aims to develop a bioactive, bioprinted contact lens composed of polyvinyl alcohol (PVA), hyaluronic acid (HA), and conditioned medium clerived from umbilical cord mesenchymal stem/stromal cells (UC-lv!SCsw), hich contains the secretome. The purpose is to provide an effective alternative to conventional therapies by supporting corneal healing and regeneration. The proposed contact lens is designed to deliver bioactive molecules in a sustained manner via the secretome, while HA incorporation aims to replicate the hydration and mechanical properties of the native cornea, ensuring comfort and biological functionality. Using the BioX bioprinter, a transparent and flexible lens will be fabricated by printing a customized bioink and stabilizing it through a hybricl crosslinking strategy that combine ionic crosslinking and freeze-thaw cycling. Comprehensive characterization of the final hyclrogel material will be performed through physicochemical tests. These analyses include Fourier Transform lnfrared Spectroscopy (FTIR), for chemical characterization, Scanning Electron lvlicroscopy (SEM) for morphological assessment, and swelling and degradation assays to evaluate hydrogel behavior under physiological conditions. Furthermore, cytocompatibility and regenerative potential will be evaluated via in vitro assays, inclucling Presto Blue1v1, Live/Deacl viability assay, and scratch assay for cell migration. This study proposes a novel, non-invasive therapeutic approach to enhance corneal wound healing, reduce fibrosis, and improve visual outcomes. Future work will include in vivo validation in rat model to confirm the efficacy and biocompatibility of the bioactive lens system.
Descrição
Palavras-chave
Bioprinting Corneal repair Secretome Umbilical Cord Mesenchymal Stem/Stromal Cells
Contexto Educativo
Citação
Pereira, M., Silva, M., Sousa, A. C., Alvites, R., Araújo, A., Delgado, E., & Maurício, A. C. (2025). PVA-based contact lenses incorporating Hyaluronic Acid and secretome for corneal repair. Book of Abstracts of the 7th Meeting on Medicinal Biotechnology - 3rd Iberian Congress on Medicinal Biotechnology, 24.