Publication
Signature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activation
| dc.contributor.author | Inácio, Daniel | |
| dc.contributor.author | Amado, Tiago | |
| dc.contributor.author | Pamplona, Ana | |
| dc.contributor.author | Sobral, Daniel | |
| dc.contributor.author | Cunha, Carolina | |
| dc.contributor.author | Santos, Rita F. | |
| dc.contributor.author | Oliveira, Liliana | |
| dc.contributor.author | Rouquié, Nelly | |
| dc.contributor.author | Carmo, Alexandre M. | |
| dc.contributor.author | Lesourne, Renaud | |
| dc.contributor.author | Gomes, Anita Q. | |
| dc.contributor.author | Santos, Bruno Silva | |
| dc.contributor.author | Santos, Ana Rita | |
| dc.date.accessioned | 2026-01-14T11:54:00Z | |
| dc.date.available | 2026-01-14T11:54:00Z | |
| dc.date.issued | 2025-01-29 | |
| dc.description.abstract | γδ T cells producing either interleukin-17A (γδ17 cells) or interferon-γ (γδIFN cells) are generated in the mouse thymus, but the molecular regulators of their peripheral functions are not fully characterized. Here we established an Il17a-GFP:Ifng-YFP double-reporter mouse strain to analyze at unprecedented depth the transcriptomes of pure γδ17 cell versus γδIFN cell populations from peripheral lymph nodes. Within a very high fraction of differentially expressed genes, we identify a panel of 20 new signature genes in steady-state γδ17 cells versus γδIFN cells, which we further validate in models of experimental autoimmune encephalomyelitis and cerebral malaria, respectively. Among the signature genes, we show that the co-receptor CD6 and the signaling protein Themis promote the activation and proliferation of peripheral γδIFN cells in response to T cell antigen receptor stimulation in vitro and to Plasmodium infection in vivo. This resource can help to understand the distinct activities of effector γδ T cell subsets in pathophysiology. | eng |
| dc.identifier.citation | Inácio, D., Amado, T., Pamplona, A., Sobral, D., Cunha, C., Santos, R. F., Oliveira, L., Rouquié, N., Carmo, A. M., Lesourne, R., Gomes, A. Q., & Silva-Santos, B. (2025). Signature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activation. Nature Immunology, 26(3), 497–510. https://doi.org/10.1038/s41590-024-02073-8 | |
| dc.identifier.doi | 10.1038/s41590-024-02073-8 | |
| dc.identifier.eissn | 1529-2908 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/31514 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Springer Nature | |
| dc.relation.hasversion | https://www.nature.com/articles/s41590-024-02073-8 | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | γδ T cells | |
| dc.subject | interleukin-17A (γδ17 cells) | |
| dc.subject | interferon-γ (γδIFN cells) | |
| dc.title | Signature cytokine-associated transcriptome analysis of effector γδ T cells identifies subset-specific regulators of peripheral activation | eng |
| dc.type | research article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 510 | |
| oaire.citation.startPage | 497 | |
| oaire.citation.title | Nature immunology | |
| oaire.citation.volume | 26 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Santos | |
| person.givenName | Ana Rita | |
| person.identifier.ciencia-id | 0911-4138-0C3A | |
| person.identifier.orcid | 0000-0003-0139-0285 | |
| relation.isAuthorOfPublication | d71931b8-d869-4334-993c-ba14ad713f01 | |
| relation.isAuthorOfPublication.latestForDiscovery | d71931b8-d869-4334-993c-ba14ad713f01 |