Bioaugmentation with Endophytic Bacterium E6S Homologous to Achromobacter piechaudii Enhances Metal Rhizoaccumulation in Host Sedum plumbizincicola

Ma, Ying; Zhang, Chang; Oliveira, Rui S.; Freitas, Helena; Luo, Yongming

Publication

Bioaugmentation with Endophytic Bacterium E6S Homologous to Achromobacter piechaudii Enhances Metal Rhizoaccumulation in Host Sedum plumbizincicola

2016Journal article

dc.contributor.author	Ma, Ying
dc.contributor.author	Zhang, Chang
dc.contributor.author	Oliveira, Rui S.
dc.contributor.author	Freitas, Helena
dc.contributor.author	Luo, Yongming
dc.date.accessioned	2017-01-17T14:43:44Z
dc.date.available	2017-01-17T14:43:44Z
dc.date.issued	2016
dc.description.abstract	Application of hyperaccumulator-endophyte symbiotic systems is a potential approach to improve phytoremediation efficiency, since some beneficial endophytic bacteria are able to detoxify heavy metals, alter metal solubility in soil, and facilitate plant growth. The objective of this study was to isolate multi-metal resistant and plant beneficial endophytic bacteria and to evaluate their role in enhancing plant growth and metal accumulation/translocation. The metal resistant endophytic bacterial strain E6S was isolated from stems of the Zn/Cd hyperaccumulator plant Sedum plumbizincicola growing in metalliferous mine soils using Dworkin and Foster salts minimal agar medium with 1-aminocyclopropane-1-carboxylate (ACC) as the sole nitrogen source, and identified as homologous to Achromobacter piechaudii based on morphological and biochemical characteristics, partial 16S rDNA sequence and phylogenetic analysis. Strain E6S showed high level of resistance to various metals (Cd, Zn, and Pb). Besides utilizing ACC, strain E6S exhibited plant beneficial traits, such as solubilization of phosphate and production of indole-3-acetic acid. Inoculation with E6S significantly increased the bioavailability of Cd, Zn, and Pb in soil. In addition, bacterial cells bound considerable amounts of metal ions in the following order: Zn > Cd >Pb. Inoculation of E6S significantly stimulated plant biomass, uptake and bioaccumulation of Cd, Zn, and Pb. However, E6S greatly reduced the root to shoot translocation of Cd and Zn, indicating that bacterial inoculation assisted the host plant to uptake and store heavy metals in its root system. Inoculation with the endophytic bacterium E6S homologous to A. piechaudii can improve phytostabilization of metalliferous soils due to its effective ability to enhance in situ metal rhizoaccumulation in plants.	pt_PT
dc.description.version	info:eu-repo/semantics/publishedVersion	pt_PT
dc.identifier.doi	10.3389/fpls.2016.00075	pt_PT
dc.identifier.issn	1664-462X
dc.identifier.uri	http://hdl.handle.net/10400.22/9288
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Frontiers	pt_PT
dc.relation.publisherversion	http://journal.frontiersin.org/article/10.3389/fpls.2016.00075/full	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Endophyticbacterium,,,	pt_PT
dc.subject	Rhizoaccumulation	pt_PT
dc.subject	Phytostabilization	pt_PT
dc.subject	Sedum plumbizincicola	pt_PT
dc.subject	Multi-metalcontamination	pt_PT
dc.title	Bioaugmentation with Endophytic Bacterium E6S Homologous to Achromobacter piechaudii Enhances Metal Rhizoaccumulation in Host Sedum plumbizincicola	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.citation.endPage	9	pt_PT
oaire.citation.issue	75	pt_PT
oaire.citation.startPage	1	pt_PT
oaire.citation.title	Frontiers in Plant Science	pt_PT
oaire.citation.volume	7	pt_PT
person.familyName	Oliveira
person.givenName	Rui S.
person.identifier.ciencia-id	251B-951A-F21E
person.identifier.orcid	0000-0001-5252-5595
person.identifier.scopus-author-id	56865275400
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isAuthorOfPublication	ddc0cf48-bab2-4711-a6f6-aa5dc7b7295d
relation.isAuthorOfPublication.latestForDiscovery	ddc0cf48-bab2-4711-a6f6-aa5dc7b7295d