Decoding immunomodulatory interactions: Exploring the crosstalk between bone marrow-derived mesenchymal steam cells (BM-MSCs) out the bag and peripheral blood mononuclear cells (PBMCs) for potential in-vivo immunosuppresion

Monteiro, Raquel Leão

Publication

Decoding immunomodulatory interactions: Exploring the crosstalk between bone marrow-derived mesenchymal steam cells (BM-MSCs) out the bag and peripheral blood mononuclear cells (PBMCs) for potential in-vivo immunosuppresion

2024-07-22Master thesis

dc.contributor.advisor	Lopes, Sérgio Manuel Bernardo Machado
dc.contributor.advisor	Vieira, Ana Filipa Quintela
dc.contributor.author	Monteiro, Raquel Leão
dc.date.accessioned	2025-03-06T13:45:48Z
dc.date.available	2025-03-06T13:45:48Z
dc.date.issued	2024-07-22
dc.date.submitted	2024-07-22
dc.description.abstract	Allogenic hematopoietic stem cell transplant (Allo-HSCT) is crucial for hematological diseases, yet chronic complications may arise as donor imune cells start attacking patient tissues. A lab barrier to studying this alloreactivity is the absence of the hematopoietic stem cell (HSC) niche. Here, we employed boné marrow-derived mesenchymal stem cells (BM-MSCs) from discarded collection bags to assess their potential in modulating peripheral blood mononuclear cell (PBMC) proliferation in-vitro and decode occuring interactions to propose mechanisms of in-vivo immunomodulation. Responder PBMCs, labelled with CFSE dye, were co-cultured for 6 days with irradiated stimulator cell in a 2:1 ratio to mimic alloreactivity, with or without BM-MSCs. Flow cytometry tracked BM-MSCs phenotype and PBMC proliferation/ surface marker expression, while resazurin assays monitored metabolic activity over time. CFSE immunofluorescence and phase-contrast images recorded PBMC proliferation interactions. BM-MSCs expanded successfully, maintaining adequate phenotype. Co-cultures exhibited fewer CFSE-stained PBMCs, equating to na 18% reduction in responder cells, with enhanced metabolic activity. Immunophenotyping revealed downregulation of T cell subsets and increased PD-1 expression, suggesting immunosuppressive effects. These findings underscore BM-MSCs’ immunomodulatory potential, offering a celular therapeutic avenue. Repurposing collection bags as a source of BM-MSCs also offers a practical solution, reducing medical waste and enhancing cost efficiency.	por
dc.identifier.tid	203878728
dc.identifier.uri	http://hdl.handle.net/10400.22/29806
dc.language.iso	eng
dc.rights.uri	N/A
dc.subject	Hematological disease
dc.subject	Peripheral blood mononuclear cells
dc.subject	Bone marrow
dc.subject	Mesenchymal steam cells
dc.subject	Immunomodulation
dc.subject	Flow cytometry
dc.title	Decoding immunomodulatory interactions: Exploring the crosstalk between bone marrow-derived mesenchymal steam cells (BM-MSCs) out the bag and peripheral blood mononuclear cells (PBMCs) for potential in-vivo immunosuppresion	por
dc.type	master thesis
dspace.entity.type	Publication
thesis.degree.name	Patologia Molecular