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Anti-Angiogenic Properties of Cafestol and Kahweol Palmitate Diterpene Esters

2016Journal article Open access
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dc.contributor.authorMoeenfard, Marzieh
dc.contributor.authorCortez, Alice
dc.contributor.authorMachado, Vera
dc.contributor.authorCosta, Raquel
dc.contributor.authorLuís, Carla
dc.contributor.authorCoelho, Pedro
dc.contributor.authorSoares, Raquel
dc.contributor.authorAlves, Arminda
dc.contributor.authorBorges, Nuno
dc.contributor.authorSantos, Alejandro
dc.date.accessioned2019-07-01T13:58:52Z
dc.date.available2019-07-01T13:58:52Z
dc.date.issued2016
dc.description.abstractEpidemiological studies support the association of coffee-specific diterpenes, with various beneficial health effects. Although anti-antiangiogenic properties of free cafestol and kahweol have been recently described, available data regarding their esterified form, in particular palmitate esters as the main diterpene esters present in coffee, are still rare. Given that angiogenesis plays an important role in many pathological conditions, including cancer growth and metastasis, this study aimed to assess and compare the potential anti-angiogenic effects of cafestol palmitate (CP) and kahweol palmitate (KP) in an in vitro angiogenesis model. According to our findings, both compounds inhibited angiogenesis steps on human microvascular endothelial cells (HMVECs), although a more significant effect was observed for KP. Compared to control, HMVECs viability decreased in a dose-dependent manner upon incubation either with CP or KP. Concentrations of 75 and 100 μM of each compound were cytotoxic. Cell proliferation was also dramatically reduced by both diterpene esters at 50 μM, although KP had a stronger inhibitory effect. However, CP and KP did not induce apoptosis on HMVECs. Both compounds reduced cell migration, but this effect was only statistically significant after KP incubation. Inhibition of VEGFR2 expression and its downstream effector Akt, but not Erk, was also observed in CP- and KP-treated HMVECs. These findings were confirmed using ELISA assay for phosphorylated (active) VEGFR-2. Taken together, these data indicate that both CP and KP can be considered potent compounds against angiogenesis-dependent disorders. Our findings further indicate that KP exerts more potent anti-angiogenic effects than CP, in most of assays.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMoeenfard, M., Cortez, A., Machado, V., Costa, R., Luís, C., Coelho, P., Soares, R., Alves, A., Borges, N., & Santos, A. (2016). Anti-Angiogenic Properties of Cafestol and Kahweol Palmitate Diterpene Esters. Journal of Cellular Biochemistry, 117(12), 2748–2756. https://doi.org/10.1002/jcb.25573
dc.identifier.doi10.1002/jcb.25573pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/14188
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationID/EQU/00511/2013-LEPABEpt_PT
dc.relationINCIDENCE AND HEALTH EFFECTS OF CAFESTOL AND KAHWEOL IN COFFEE BREWS PREPARED BY RECENT TECHNOLOGY
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/jcb.25573pt_PT
dc.subjectAngiogenesis Inhibitorspt_PT
dc.subjectApoptosispt_PT
dc.subjectBlotting, Westernpt_PT
dc.subjectCell Movementpt_PT
dc.subjectCell Proliferationpt_PT
dc.subjectDiterpenespt_PT
dc.subjectEsterspt_PT
dc.subjectHuman Umbilical Vein Endothelial Cellspt_PT
dc.subjectHumanspt_PT
dc.subjectNeovascularization, Physiologicpt_PT
dc.subjectPhosphorylationpt_PT
dc.subjectTumor Cells, Culturedpt_PT
dc.subjectVascular Endothelial Growth Factor Apt_PT
dc.subjectVascular Endothelial Growth Factor Receptor-2pt_PT
dc.titleAnti-Angiogenic Properties of Cafestol and Kahweol Palmitate Diterpene Esterspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleINCIDENCE AND HEALTH EFFECTS OF CAFESTOL AND KAHWEOL IN COFFEE BREWS PREPARED BY RECENT TECHNOLOGY
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04293%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F79318%2F2011/PT
oaire.citation.endPage2756pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage2748pt_PT
oaire.citation.titleJournal of Cellular Biochemistrypt_PT
oaire.citation.volume117pt_PT
oaire.fundingStream5876
person.familyNameCoelho
person.givenNamePedro
person.identifier.ciencia-idBE18-3DB2-8D79
person.identifier.orcid0000-0002-2343-1108
person.identifier.ridAAZ-9239-2020
person.identifier.scopus-author-id57214054449
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationeb030614-4140-437b-8ac3-f59c52566ecf
relation.isAuthorOfPublication.latestForDiscoveryeb030614-4140-437b-8ac3-f59c52566ecf
relation.isProjectOfPublicationa21beac1-88e1-4514-a951-aee8f9dd8cfc
relation.isProjectOfPublication4521eced-8190-467e-883c-49bf0a3fda67
relation.isProjectOfPublication.latestForDiscoverya21beac1-88e1-4514-a951-aee8f9dd8cfc

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