Publication
Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD
| dc.contributor.author | Marques, Raquel C.B. | |
| dc.contributor.author | Costa Rama, Estefanía | |
| dc.contributor.author | Viswanathan, Subramanian | |
| dc.contributor.author | Nouws, Henri | |
| dc.contributor.author | Costa-García, Agustín | |
| dc.contributor.author | Delerue-Matos, Cristina | |
| dc.contributor.author | González-García, M. Begoña | |
| dc.date.accessioned | 2019-10-07T13:41:28Z | |
| dc.date.available | 2019-10-07T13:41:28Z | |
| dc.date.issued | 2018 | |
| dc.description.abstract | Cancer Antigen 15-3 (CA 15-3) and the extracellular domain of the human epidermal growth factor receptor 2 (HER2-ECD) are independent breast cancer biomarkers. The combination of their profiles (presence and concentration) could provide an important contribution to diagnostics and patient follow-up. Therefore, a disposable electrochemical immunosensor for the simultaneous detection of CA 15-3 and HER2-ECD was developed in this work. The immunosensor was constructed on a customized dual screen-printed carbon electrode. The carbon working electrodes' surfaces were first modified with in situ electrodeposited gold nanoparticles and then individually coated with either a monoclonal anti-human CA 15-3 or a monoclonal anti-human HER2-ECD antibody. After incubation with the biomarkers and monoclonal biotin-labelled detection antibodies, the antigen-antibody interactions were detected by linear sweep voltammetric analysis of enzymatically (alkaline phosphatase) generated metallic silver. The immunosensor’s limits of detection for the selected biomarkers were 5.0 U mL−1 for CA 15-3 and 2.9 ng mL−1 for HER2-ECD. These values could allow the use of the sensor in the non-invasive control of these biomarkers in breast cancer patients. | pt_PT |
| dc.description.sponsorship | This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT, Fundação para a Ciência e a Tecnologia) through projects PTDC/SAU-ENB/114786/2009 and UID/QUI/50006/2013. Estefanía Costa Rama thanks the Government of Principado de Asturias for the award of a Severo Ochoa predoctoral grant (BP11-097). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.doi | 10.1016/j.snb.2017.08.107 | |
| dc.identifier.issn | 0925-4005 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/14706 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier | pt_PT |
| dc.relation | Nano-electrode arrays Biosensor for Early and Decentralized Breast-Cancer Diagnosis | |
| dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0925400517315307?via%3Dihub | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
| dc.subject | Multiplexed immunosensor | pt_PT |
| dc.subject | Electrochemical immunosensor | pt_PT |
| dc.subject | Breast cancer biomarker | pt_PT |
| dc.subject | CA 15-3 | pt_PT |
| dc.subject | HER2-ECD | pt_PT |
| dc.title | Voltammetric immunosensor for the simultaneous analysis of the breast cancer biomarkers CA 15-3 and HER2-ECD | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Nano-electrode arrays Biosensor for Early and Decentralized Breast-Cancer Diagnosis | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FSAU-ENB%2F114786%2F2009/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PT | |
| oaire.citation.endPage | 925 | pt_PT |
| oaire.citation.startPage | 918 | pt_PT |
| oaire.citation.title | Sensors and Actuators B: Chemical | pt_PT |
| oaire.citation.volume | 255 | pt_PT |
| oaire.fundingStream | 5876-PPCDTI | |
| oaire.fundingStream | 5876 | |
| person.familyName | Costa Rama | |
| person.familyName | Nouws | |
| person.familyName | Delerue-Matos | |
| person.givenName | Estefanía | |
| person.givenName | Hendrikus | |
| person.givenName | Cristina | |
| person.identifier | 1046746 | |
| person.identifier.ciencia-id | 3614-E403-0136 | |
| person.identifier.ciencia-id | 9B1A-9CED-6EE3 | |
| person.identifier.ciencia-id | 9A1A-43FB-5C27 | |
| person.identifier.orcid | 0000-0002-5357-9269 | |
| person.identifier.orcid | 0000-0002-1472-8866 | |
| person.identifier.orcid | 0000-0002-3924-776X | |
| person.identifier.rid | J-5815-2018 | |
| person.identifier.rid | D-5249-2013 | |
| person.identifier.rid | D-4990-2013 | |
| person.identifier.scopus-author-id | 36703129800 | |
| person.identifier.scopus-author-id | 7801450247 | |
| person.identifier.scopus-author-id | 6603741848 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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